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SKU BA1001
Pack size 0.5 ml
Applications ELISA, IHC-P, IHC-F, ICC, WB

Product Overview

Product Name Goat Anti-Mouse IgG (H+L) Secondary Antibody, Biotin Conjugate
Synonyms Biotin-conjugated goat anti-mouse IgG; Goat Anti-Mouse IgG Biotinylated Antibody; Biotinylated Goat Anti-Mouse IgG Secondary Antibody
Description Goat Anti-Mouse IgG (H+L) Secondary Antibody, Biotin Conjugate, for indirect sensitive immunodetection and/or quantification of low-abundancy target proteins through ELISA, IHC-P, IHC-F, ICC, or WB
Label Biotin
Host Goat
Target Species Mouse
Antibody Class IgG
Clonality Polyclonal
Immunogen Whole molecule mouse IgG
Purification Immunoaffinity chromatography
Specificity Mouse IgG specific; no cross-reactivity with human/bovine/rabbit IgG
Form Supplied Liquid: concentrated buffered stock solution
Formulation 0.5 mg biotin-conjugated secondary antibody
0.01 M PBS (PH 7.4)
50% glycerol
Pack Size 0.5 ml
Concentration 1 mg/ml
Application ELISA, IHC-P, IHC-F, ICC, WB
Storage 4C for 1 year
Precautions FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE

Assay Information

Sample Type SDS-PAGE separated-, membrane-immobilized-, mouse primary-antibody-probed proteins from cell/tissue lysates
Assay Type Immunoassay
Assay Purpose Protein detection/quantification
Technique Immunodetection of target protein via reporter-labeled biotin-binding (detection) systems
Equipment Needed WB/Dot blot/ELISA/IHC instrumentation; X-ray film cassette or a charge-coupled device (CCD) imager; Spectrophotometer; fluorescent or electron microscope

Main Advantages

Specific High signal-to-noise ratio
Sensitive High reporter-to-antibody ratio
High Signal Amplification Multiple secondary antibodies can bind to a single primary antibody;Secondary antibodies Fc regions provide further binding locations for biotin, or enable the use of ABC and SABC
Fast Generates strong signals in a relatively short time span
Quantifieable Allows quantification of detected signal
Easy to Use Supplied in a workable liquid format
Flexible Biotin-Avidin system can be coupled with various types of reporters (enzymes, fluorochromes, fluorophores, chromophores, etc.)
Convenient Biotin's small size renders no interference with enzymatic catalysis or antibody binding

Background

Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest, and then modified with antibody fragmentation, label conjugation, etc. to achieve highly specific reagents for molecular detection. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest by recognizing a primary antibody that targets a particular antigen, and the label generates a detectable signal in the area of the formed immune complexes. The label of choice depends upon the experimental application.
Biotinylated antibodies are widely used in systems where signal amplification is desired. Often 15-20 biotin moieties are coupled to a single IgG secondary antibody. Biotin binds avidin, streptavidin, or neutravidin with a high degree of affinity and specificity. In immunoassays avidin/streptavidin-biotin binding is used as a bridge between antibodies and reporters like enzymes (HRP, AP), fluorophores, chromophores, etc. Both avidin and streptavidin are tetrameric proteins capable of binding 4 biotin groups to each molecule of avidin or streptavidin, thus amplifying the signal intensity and detection sensitivity by increasing the concentration of reporters at the antigenic site. Two main biotin-binding detection systems have been widely utilized: Avidin-Biotin Complex (ABC) and Labeled Streptavidin Biotin (LSAB) methods. In the ABC method free avidin (or streptavidin) is used as a bridge/link between the biotinylated antibody and а biotinylated reporter molecule, resulting in three reporter molecules coupled to the biotinylated antibody. The LSAB method employs a reporter-labeled streptavidin (avidin or neutravidin can alternatively be used) to detect the bound biotinylated-secondary antibody on the tissue section, blotting membrane or ELISA plate, improving the sensitivity of detection by 8-fold. The LSAB method is used when the avidin-biotin-enzyme complex in the ABC method becomes too large to penetrate the tissue.

Goat Anti-Mouse IgG (H+L) Secondary Antibody, Biotin Conjugate Images

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Goat Anti-Mouse IgG (H+L) Secondary Antibody, Biotin Conjugate
NEFH was detected in paraffin-embedded sections of rat brain tissues using mouse anti-NEFH Antigen Affinity purified monoclonal antibody (Catalog # MA1071) at 1 ??g/mL. Biotin Conjugated goat anti-mouse IgG (H+L) secondary antibody (Catalog # BA1001) was used to detect the primary antibody at 10??g/mL.
Goat Anti-Mouse IgG (H+L) Secondary Antibody, Biotin Conjugate
PCNA was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti- PCNA Antigen Affinity purified monoclonal antibody (Catalog # MA1083) at 1 μg/mL. Biotin Conjugated goat anti-mouse IgG (H+L) secondary antibody (Catalog # BA1001) was used to detect the primary antibody at 10μg/mL.
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Publications

TIM-4 blockade of KCs combined with exogenous TGF-? injection helps to reverse acute rejection and prolong the survival rate of mice receiving liver allografts
Colon cancers carrying BRAF V600E and ?-catenin T41A activating mutations are resistant to numerous common anticancer drugs
TRP-2 mediates coat color pigmentation in sheep skin
Synovial fluid-derived synovial fragments represent an improved source of synovial mesenchymal stem cells in the temporomandibular joint
Iguratimod prevents ovariectomy-induced bone loss and suppresses osteoclastogenesis via inhibition of peroxisome proliferator-activated receptor-?
CD146+ skeletal stem cells from growth plate exhibit specific chondrogenic differentiation capacity in vitro
SPOCK1 promotes the invasion and metastasis of gastric cancer through Slug?induced epithelial?mesenchymal transition
Metadherin regulates actin cytoskeletal remodeling and enhances human gastric cancer metastasis via epithelial-mesenchymal transition
Inhibitory effects of S100A4 gene silencing on alkali burn-induced corneal neovascularization: an in vivo study
Bacillus Calmette-Guerin alleviates airway inflammation and remodeling by preventing TGF-?1 induced epithelial?mesenchymal transition
A Clinical Trial Using Attrition Combined with 5-Aminolevulinic Acids Based Photodynamic Therapy in Treating Squamous Cell Carcinoma
Dimethyloxaloylglycine increases bone repair capacity of adipose-derived stem cells in the treatment of osteonecrosis of the femoral head
Expression and prognostic significance of CTBP2 in human gliomas
Role of rat autologous skin fibroblasts and mechanism underlying the repair of depressed scars
Inhibition of the NMDA receptor protects the rat sciatic nerve against ischemia/reperfusion injury
Differential expression of EBV proteins LMP1 and BHFR1 in EBV?associated gastric and nasopharyngeal cancer tissues
Effect of interleukin-2 treatment combined with magnetic fluid hyperthermia on Lewis lung cancer-bearing mice
Clinical significance and prognostic value of Nek2 protein expression in colon cancer
Effect of Diclazuril on the Bursa of Fabricius Morphology and SIgA Expression in Chickens Infected with?Eimeria tenella
Bak Foong pills combined with metformin in the treatment of a polycystic ovarian syndrome rat model
Nickel chloride (NiCl2)-caused inflammatory responses via activation of NF-?B pathway and reduction of anti-inflammatory mediator expression in the kidney
Modulation of the PI3K/Akt Pathway and Bcl-2 Family Proteins Involved in Chicken?s Tubular Apoptosis Induced by Nickel Chloride (NiCl2)
Overexpression of caudal-related homeobox transcription factor 2 inhibits the growth of transplanted colorectal tumors in nude mice
Exogenous Nkx2.5- or GATA-4-transfected rabbit bone marrow mesenchymal stem cells and myocardial cell co-culture on the treatment of myocardial infarction in rabbits
Buyuan Congnao decoction decreases hippocampal beta-amyloid expression in a rat model of Alzheimer's disease
Comparison of immune responses to intranasal and intrapulmonary vaccinations with the attenuated Mycoplasma hyopneumoniae 168 strain in pigs
MiR-194, commonly repressed in colorectal cancer, suppresses tumor growth by regulating the MAP4K4/c-Jun/MDM2 signaling pathway
Adenosine triphosphate promotes locomotor recovery after spinal cord injury by activating mammalian target of rapamycin pathway in rats
Co-expression of perforin and granzyme B genes induces apoptosis and inhibits the tumorigenicity of laryngeal cancer cell line Hep-2
Function of the transforming growth factor-?1/c-Jun N-terminal kinase signaling pathway in the action of thalidomide on a rat model of pulmonary fibrosis
Matrix metalloproteinase-9 overexpression is closely related to poor prognosis in patients with colon cancer
Effects of Constitutive ?-Catenin Activation on Vertebral Bone Growth and Remodeling at Different Postnatal Stages in Mice
Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice
Porous Chitosan Scaffolds with Embedded Hyaluronic Acid/Chitosan/Plasmid-DNA Nanoparticles Encoding TGF-?1 Induce DNA Controlled Release, Transfected Chondrocytes, and Promoted Cell Proliferation
Hyperglycemia induces endoplasmic reticulum stress-dependent CHOP expression in osteoblasts
Iptakalim, an ATP-sensitive potassium channel opener, confers neuroprotection against cerebral ischemia/reperfusion injury in rats by protecting neurovascular unit cells
Heat shock protein 70 inhibits hydrogen peroxide-induced nucleolar fragmentation via suppressing cleavage and down-regulation of nucleolin
Activin B Promotes Epithelial Wound Healing In Vivo through RhoA-JNK Signaling Pathway
Oxidative stress and hypoxia-induced factor 1? expression in gastric ischemia
Molecular structure of the largemouth bass (Micropterus salmoides) Myf5 gene and its effect on skeletal muscle growth
LIF Upregulates Expression of NK-1R in NHBE Cells
Correlation of?p53?gene mutation and expression of P53 protein in cholangiocarcinoma
In vivo?effects of Chinese herbal recipe, Danshaohuaxian, on apoptosis and proliferation of hepatic stellate cells in hepatic fibrotic rats
Construction of HCV-core gene vector and its expression in cholangiocarcinoma
Expression pattern of neural synaptic plasticity marker-Arc in different brain regions induced by conditioned drug withdrawal from acute morphine-dependent rats
Limbic Epileptogenesis in a Mouse Model of Fragile X Syndrome
Promotion of the articular cartilage proteoglycan degradation by T-2 toxin and selenium protective effect\n
Iptakalim, an ATP-sensitive potassium channel opener, confers neuroprotection against cerebral ischemia/reperfusion injury in rats by protecting neurovascular unit cells
Heat shock protein 70 inhibits hydrogen peroxide-induced nucleolar fragmentation via suppressing cleavage and down-regulation of nucleolin
Activin B Promotes Epithelial Wound Healing In Vivo through RhoA-JNK Signaling Pathway
Oxidative stress and hypoxia-induced factor 1? expression in gastric ischemia
Molecular structure of the largemouth bass (Micropterus salmoides) Myf5 gene and its effect on skeletal muscle growth
LIF Upregulates Expression of NK-1R in NHBE Cells
Correlation of
Construction of HCV-core gene vector and its expression in cholangiocarcinoma
Expression pattern of neural synaptic plasticity marker-Arc in different brain regions induced by conditioned drug withdrawal from acute morphine-dependent rats
Limbic Epileptogenesis in a Mouse Model of Fragile X Syndrome
Promotion of the articular cartilage proteoglycan degradation by T-2 toxin and selenium protective effect\n
Du Y1, Jiang B1, Song S1, Pei G1, Ni X1, Wu J1, Wang S1, Wang Z2, Yu J1. Int J Oncol. 2017 Jul;51(1):63-74. doi: 10.3892/ijo.2017.4002. Epub 2017 May 17. Metadherin regulates actin cytoskeletal remodeling and enhances human gastric cancer metastasis via epithelial-mesenchymal transition
Chen D, Zhou H, Liu G, Zhao Y, Cao G, Liu Q. J Cell Mol Med. 2018 Feb;22(2):797-807. doi: 10.1111/jcmm.13357. Epub 2017 Sep 22. SPOCK1 promotes the invasion and metastasis of gastric cancer through Slug
Wu YX, Sun Y, Ye YP, Zhang P, Guo JC, Huang JM, Jing XZ, Xiang W, Yu SY, Guo FJ. Mol Med Rep. 2017 Dec;16(6):8200-8208. doi: 10.3892/mmr.2017.7648. Epub 2017 Sep 28. Iguratimod prevents ovariectomy-induced bone loss and suppresses osteoclastogenesis via inhibition of peroxisome proliferator-activated receptor-γ
Peng J, Feng W, Luo X, Wang T, Xiang W, Dai Y, Zhu J, Zheng J. Med Sci Monit. 2017 Mar 18;23:1347-1354. A Clinical Trial Using Attrition Combined with 5-Aminolevulinic Acids Based Photodynamic Therapy in Treating Squamous Cell Carcinoma
Yao Y, Li ZY, Zhang H, Zheng YH, Mai LX, Liu WJ, Zhang ZG, Sun YP. Int J Mol Med. 2018 Jan;41(1):173-183. doi: 10.3892/ijmm.2017.3210. Epub 2017 Oct 25. Synovial fluid-derived synovial fragments represent an improved source of synovial mesenchymal stem cells in the temporomandibular joint
Xue L, Li Y, Zhao B, Chen T, Dong Y, Fan R, Li J, Wang H, He X. Mol Med Rep. 2018 Apr;17(4):5869-5877. doi: 10.3892/mmr.2018.8563. Epub 2018 Feb 6. TRP-2 mediates coat color pigmentation in sheep skin
Xu G, Li K, Zhang N, Zhu B, Feng G, Fan Q. Oncol Lett. 2018 Apr;15(4):4471-4476. doi: 10.3892/ol.2018.7856. Epub 2018 Jan 25. Colon cancers carrying BRAF V600E and β-catenin T41A activating mutations are resistant to numerous common anticancer drugs
Yalin Jiang Yunfeng Zhou Yufeng Zheng Hong Guo Lei Gao Pan Chen Dandan Feng Ran Qi Xiaozhen Li Yongchao Chang Fong‑Fong Chu Qiang Gao DOI: 10.3892/ol.2017.5590, Online ISSN:1792-1082 Expression of inositol‑requiring enzyme 1β is downregulated in colorectal cancer
Zhonghua Li, Fangzhou Chen, Shiyi Ye, Xiaozhen Guo, Atta Muhanmmad Memon, Meizhou Wu and Qigai He Viruses 2016, 8(12), 323; doi: 10.3390/v8120323 Comparative Proteome Analysis of Porcine Jejunum Tissues in Response to a Virulent Strain of Porcine Epidemic Diarrhea Virus and Its Attenuated Strain