HRP Conjugated AffiniPure Goat Anti-Mouse IgG (H+L)

This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations. Cited in 10 publication(s).

Product Info Summary

SKU: BA1050
Size: 0.5ml
Reactive Species: Mouse
Host: Goat
Application: ELISA, WB

Product Overview

Product Name HRP Conjugated AffiniPure Goat Anti-Mouse IgG (H+L)
Synonyms HRP-conjugated goat anti-mouse IgG
Description HRP Conjugated Goat Anti-mouse IgG (H+L) (gamma-chain specific) secondary antibody This HRP conjugated antibody is specific for mouse IgG and shows no cross-reactivity with human/bovine/rabbit IgG.
Reagent Type Secondary antibody, reporter enzyme labeled
Label HRP (Horseradish Peroxidase)
Host Goat
Target Species Mouse
Antibody Class IgG
Clonality Polyclonal
Immunogen Mouse IgG (whole molecular)
Preparation Affinity purified from rabbit antiserum
Specificyity This HRP conjugated antibody is specific for mouse IgG and shows no cross-reactivity with human/bovine/rabbit IgG.
Form Supplied Concentrated, Liquid
Formulation 0.5 mg of HRP conjugated specific antibody
0.01 M PBS (pH7.4)
50% glycerol
Pack Size 0.5 ml
Concentration 1mg/ml
Application ELISA*, WB*
*Our Boster Guarantee covers the use of this product in the above marked tested applications.
Storage At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
Precautions FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE

Assay Information

Sample Type SDS-PAGE separated-, membrane-immobilized-, mouse primary-antibody-probed proteins from cell/tissue lysates
Assay Type Immunoassay
Assay Purpose Protein detection/quantification
Technique Immunodetection of target antibody with HRP reporter enzyme
Equipment Needed WB/ELISA instrumentation; X-ray film cassette or a charge-coupled device (CCD) imager; Spectrophotometer
Compatibility with Reagents Incompatible with sodium azide and metals
incompatible with high phosphate concentrations

Main Advantages

Specific High signal-to-noise ratio
Sensitive Detects low-abundant targets due to an optimal number of HRP molecules per antibody
High Signal Amplification Multiple secondary antibodies can bind to a single primary antibody;Secondary antibodies Fc regions provide further binding locations for biotin, or enable the use of ABC and SABC
Fast Generates strong signals in a relatively short time span
Quantifieable Allows quantification of detected signal
Easy to Use Supplied in a workable liquid format
Flexible HRP: compatible with chromogenic, fluorogenic and chemiluminescent substrates;
Convenient HRP’s small size: no interference with the primary/secondary antibody interaction; no steric hindrance to antibody/antigen complexes

Background

Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest. Purified secondary antibodies are further solid phase adsorbed with other species serum proteins to minimize cross-reactivity in tissue or cell preparations, and are then modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application.

Horseradish peroxidase (HRP) is extensively used for labeling secondary antibodies in ELISA, western blot, dot blot and immunohistochemistry. The HRP enzyme is made visible using a substrate that, when oxidized by HRP in the presence of hydrogen peroxide as an oxidizing agent, yields a characteristic change that is detectable by specific detection methods. The substrates commonly used with HRP fall into different categories including chromogenic, fluorogenic, and chemiluminescent substrates depending on whether they produce a colored, fluorimetric or luminescent derivative respectively. The intensity of the signal is proportional to peroxidase activity and is a measure of the number of enzyme molecules reacting, hence of the amount of recognized primary antibodies, and thus of the amount of target antigen.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western Blotting: 0.1-0.2μg/ml (ECLdetection)
Western Blotting: 0.7-3.3μg/ml (DAB detection)
Direct ELISA: 0.05-0.5μg/ml (TMB detection)

Validation Images & Assay Conditions

BA1050 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Morphologic and histopathologic change of sodium iodate-induced retinal degeneration in adult rats

Hypoxia promotes epithelial-mesenchymal transition of hepatocellular carcinoma cells via inducing Twist1 expression.

MicroRNA-9 is a ponderable index for the prognosis of human hepatocellular carcinoma

Genes associated with sodium fluoride-induced human osteoblast apoptosis

Atorvastatin ameliorates myocardial ischemia/reperfusion injury through attenuation of endoplasmic reticulum stress-induced apoptosis

Overexpression of 15-lipoxygenase-1 in oxygen-induced ischemic retinopathy inhibits retinal neovascularization via downregulation of vascular endothelial growth factor-A expression

miR-375 enhances palmitate-induced lipoapoptosis in insulin-secreting NIT-1 cells by repressing myotrophin (V1) protein expression

Study of Sequential Histopathologic Changes, Apoptosis, and Cell Proliferation in Rabbit Livers After High‐Intensity Focused Ultrasound Ablation

Serum containing Buyang Huanwu decoction prevents age-associated migration and invasion of human vascular smooth muscle cells by up regulating SIRT1 expression

Lysyl hydroxylase 3 increases collagen deposition and promotes pulmonary fibrosis by activating TGFβ1/Smad3 and Wnt/β-catenin pathways

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1 Customer Q&As for HRP Conjugated AffiniPure Goat Anti-Mouse IgG (H+L)

Question

Is this product suitable for IHC? Keyword: application, Immunohistochemistry

Verified Customer

Verified customer

Asked: 2014-12-26

Answer

Please beware that this product is not suitable for IHC. Small package of secondaries (0.5 ml) can do 15-75 slides and 1ml can do 30-150 slides. The difference is in the way customer uses the reagent.

Boster Scientific Support

Answered: 2014-12-26

Size

Total: $95

SKU:BA1050

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