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Product Info Summary
| SKU: | M01080-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-P, ICC |
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Product info
Product Name
Anti-Hu CD63 Purified Monoclonal Antibody
SKU/Catalog Number
M01080-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD63 Purified Monoclonal Antibody (Catalog# M01080-2). Tested in Flow Cytometry, IP, IHC-P, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD63 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01080-2)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
MEM-259
Isotype
Mouse IgG1
Immunogen
HPB-ALL T cell line. The antibody MEM-259 reacts with an extracellular/luminal epitope of CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.
Reactive Species
M01080-2 is reactive to CD63 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
25.6 kDa
Background of CD63
CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01080-2 is guaranteed for Flow Cytometry, IP, IHC-P, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 2 μg/ml; positive material: activated platelets, neutrophils and basophils.
Immunohistochemistry (paraffin sections): 10 μg/ml; positive tissue: spleen.
Validation Images & Assay Conditions
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Flow cytometry analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) purified, GAM-APC.
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Immunocytochemistry staining of human skin fibroblasts with anti-CD63 (MEM-259; green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).
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Immunocytochemistry staining of CD63 in human primary fibroblasts using anti-CD63 (MEM-259; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
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Immunocytochemistry staining of CD63 in human HeLa cell line using anti-CD63 (MEM-259; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
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Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD63 (TEM-259). Commercially tested by LifeSpan BioSciences.
Specific Publications For Anti-Hu CD63 Purified Monoclonal Antibody (M01080-2)
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