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Product Info Summary
| SKU: | M07865 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA |
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Product info
Product Name
Anti-Human IgG2 IGHG2 Rabbit Monoclonal Antibody, Clone#RM118
SKU/Catalog Number
M07865
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human IgG2 IGHG2 Rabbit Monoclonal Antibody, Clone#RM118 (Catalog # M07865). Tested in ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human IgG2 IGHG2 Rabbit Monoclonal Antibody, Clone#RM118 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07865)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM118
Isotype
Rabbit IgG
Immunogen
Human IgG2
Cross-reactivity
Reacts to the heavy chain of human IgG2. RM118 does not cross-react to other IgG subclasses (IgG1, IgG3, IgG4), and shows no cross-reactivity to IgM, IgA, IgD, or IgE. RM118 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Reactive Species
M07865 is reactive to IGHG2 in Human
Calculated molecular weight
35.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07865 is guaranteed for ELISA Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 50ng/well – 200ng/well (for Capture)
0.05ug/mL – 0.2ug/mL (for Detection)
Validation Images & Assay Conditions
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ELISA result showing specificity
Detection of human IgG2 in monkey serum. Sandwich ELISA using RM118 as the capture antibody, and biotinylated anti-human light chains ( or ) antibody RM129 as the detection antibody, followed by a HRP conjugated streptavidin.
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ELISA result showing specificity
Sandwich ELISA using RM118 as the capture antibody, and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin
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ELISA result showing specificity
ELISA of human immunoglobulins shows RM118 only reacted to human IgG2. No cross reactivity with Human IgG1, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. A serial dilution of RM118 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
A titer ELISA using RM118. The plate was coated with different amounts of human IgG2. A serial dilution of RM118 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
ELISA showing RM118 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM118 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
Specific Publications For Anti-Human IgG2 IGHG2 Rabbit Monoclonal Antibody, Clone#RM118 (M07865)
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Customer Q&As
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5 Customer Q&As for Anti-Human IgG2 IGHG2 Rabbit Monoclonal Antibody, Clone#RM118
Question
Is there a BSA free version of anti-Human IgG2 Rabbit Monoclonal antibody, Clone#RM118 M07865 available?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Human IgG2 Rabbit Monoclonal antibody, Clone#RM118 M07865 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-22
Question
I was wanting to use your anti-Human IgG2 Rabbit Monoclonal antibody, Clone#RM118 for ELISA for human fetal liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fetal liver identification?
Verified Customer
Verified customer
Asked: 2018-07-04
Answer
It shows on the product datasheet, M07865 anti-Human IgG2 Rabbit Monoclonal antibody, Clone#RM118 has been tested for ELISA on human tissues. We have an innovator award program that if you test this antibody and show it works in human fetal liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-04
Question
Is this M07865 anti-Human IgG2 Rabbit Monoclonal antibody, Clone#RM118 reactive to the isotypes of IGHG2?
Verified Customer
Verified customer
Asked: 2017-11-10
Answer
The immunogen of M07865 anti-Human IgG2 Rabbit Monoclonal antibody, Clone#RM118 is Human IgG2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-10
Question
I see that the anti-Human IgG2 Rabbit Monoclonal antibody, Clone#RM118 M07865 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-10-17
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-10-17
Question
We are currently using anti-Human IgG2 Rabbit Monoclonal antibody, Clone#RM118 M07865 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
J. Huang
Verified customer
Asked: 2013-04-02
Answer
The anti-Human IgG2 Rabbit Monoclonal antibody, Clone#RM118 (M07865) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-04-02
