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Product Info Summary
| SKU: | M02652 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-IFIT1 Mouse Monoclonal Antibody [Clone ID: OTI3G8]
SKU/Catalog Number
M02652
Size
100 µl
Description
Boster Bio IFIT1 mouse monoclonal antibody, clone OTI3G8 (formerly 3G8). Catalog# M02652. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-IFIT1 Mouse Monoclonal Antibody [Clone ID: OTI3G8] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02652)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI3G8
Isotype
IgG1
Immunogen
Full length human recombinant protein of human IFIT1 (NP_000899) produced in HEK293T cell.
Reactive Species
M02652 is reactive to IFIT1 in Human, Mouse, Rat
Calculated molecular weight
55.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02652 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500~1000
IHC 1:50
IF 1:50~100
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IFIT1 antibody (M02652), and then analyzed by flow cytometry.
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Anti-IFIT1 mouse monoclonal antibody (M02652) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IFIT1.
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Immunofluorescent staining of HT29 cells using anti-IFIT1 mouse monoclonal antibody (M02652).
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Immunohistochemical staining of paraffinembedded Human Kidney tissue within the normal limits using anti-IFIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02652)
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Immunohistochemical staining of paraffinembedded Human liver tissue within the normal limits using anti-IFIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02652)
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Immunohistochemical staining of paraffinembedded Carcinoma of Human liver tissue using anti-IFIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02652)
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Immunohistochemical staining of paraffinembedded Adenocarcinoma of Human ovary tissue using anti-IFIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02652)
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Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (lane 1) , human IFIT1 plasmid (lane 2), mouse IFIT1 plasmid (lane 3) using anti-IFIT1 antibody M02652 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (1:1000)
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-IFIT1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Specific Publications For Anti-IFIT1 Mouse Monoclonal Antibody [Clone ID: OTI3G8] (M02652)
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