Product Info Summary
| SKU: | A02925 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-IL-9 Antibody
SKU/Catalog Number
A02925
Size
100ug
Form
Lyophilized
Description
Boster Bio Anti-IL-9 Antibody (Catalog # A02925). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store Anti-IL-9 antibody at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship at ambient temperature.)
Cite This Product
Anti-IL-9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02925)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-9 protein.
Reactive Species
A02925 is reactive to IL9 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
15.9 kDa
Background of IL9
IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. Anti-IL-9 antibody is ideal for investigators involved in Cancer and Immunology research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02925 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000
IHC: User optimized
WB: 1:1,000
This purified antibody has been tested in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method. By western blot a band approximately 15 kDa in size corresponding to native human IL-9 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Validation Images & Assay Conditions
Click image to see more details
Boster's anti-Human IL-9 antibody shows detection of a band ~15 kDa in size corresponding to recombinant human IL-9. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).
Specific Publications For Anti-IL-9 Antibody (A02925)
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3 Customer Q&As for Anti-IL-9 Antibody
Question
I see that the anti-IL-9 antibody A02925 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-02-04
Question
We are currently using anti-IL-9 antibody A02925 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
The anti-IL-9 antibody (A02925) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-09
Question
Does anti-IL-9 antibody A02925 work on monkey WB with leukocyte?
D. Rodriguez
Verified customer
Asked: 2014-08-26
Answer
Our lab technicians have not tested anti-IL-9 antibody A02925 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-IL-9 antibody A02925 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey leukocyte in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-08-26

