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CXCR2 antibody (A00455-2) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Flow Cytometry analysis of THP-1 cells using anti-CXCR2 antibody (A00455-2).
Overlay histogram showing THP-1 cells stained with A00455-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CXCR2 Antibody (A00455-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing THP-1 cells stained with A00455-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CXCR2 Antibody (A00455-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-CXCR2 Antibody Picoband®
CXCR2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
CXCR2 antibody was positively detected by WB in:
human U20S whole cell, human A549 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CXCR2 antibody was positively detected by IHC in:
human appendicitis tissue, human tonsil tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CXCR2 antibody was positively detected by FC in:
THP-1 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-CXCR2 Antibody Picoband®
View all CXCR2 Antibodies
View all CXCR2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CXCR2 recombinant protein (Position: F32-I54;A177-R212).
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
41 kDa
Calculated Molecular Weight
40.8 kDa
Background of CXCR2
CXCR2 is a receptor for Interleukin 8, which is a powerful neutrophil chemotactic factor. It is a member of the GPCR family (subfamily, chemokine). Binding of IL8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL8 with a high affinity and to GRO/MGSA and NAP2 also with a high affinity. It has been reported to be expressed in a wide variety of tissues. ESTs have been isolated from human placenta and thymus libraries.
Cite This Product
Anti-CXCR2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00455-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
81%
Moderate Homology
Rabbit
64%
Low homology
Cow
56%
Low homology
Sheep
53%
Low homology
Mouse
47%
Low homology
Dog
47%
Low homology
Rat
44%
Low homology
Zebrafish
33%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CXCR2 antibody
Immunohistochemistry (IHC) protocol for CXCR2 antibody
Flow Cytometry (FC) protocol for CXCR2 antibody
Publications
Application:
Species:
Cerebral Endothelial CXCR2 Promotes Neutrophil Transmigration into Central Nervous System in LPS-Induced Septic Encephalopathy
Published: 2024-07-11
Journal: Biomedicines
Application: WB
Impact Factor: 3.90
Species: Mouse
PMID: 39062109
MiR-940 inhibits migration and invasion of tongue squamous cell carcinoma via regulatingCXCR2/NF-κB system-mediated epithelial–mesenchymal transition
Published: 2019-06-18
Journal: NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
Application: WB
Impact Factor: 2.06
Species: Human
PMID: 31214736
Th17 cells regulate the production of CXCL1 in breast cancer
Published: 2018-02-20
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: FC
Impact Factor: 3.12
Species: Human
PMID: 29438938
NFκB-mediated CXCL1 production in spinal cord astrocytes contributes to the maintenance of bone cancer pain in mice
Published: 2014-03-01
Journal: Journal of Neuroinflammation
Application: IHC,WB
Impact Factor: 4.90
Species: Mouse
PMID: 24580964
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Order Product
A00455-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 17, 2026