This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
IDO2 antibody (A06002-2) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Monkey, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
Filter:
Flow Cytometry analysis of K562 cells using anti-IDO-2/IDO2 antibody (A06002-2).
Overlay histogram showing K562 cells stained with A06002-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IDO-2/IDO2 Antibody (A06002-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing K562 cells stained with A06002-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IDO-2/IDO2 Antibody (A06002-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Bundle Save
Anti-IDO-2/IDO2 Antibody Picoband®
IDO2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
IDO2 antibody was positively detected by WB in:
rat liver tissue, mouse liver tissue, monkey kidney tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
IDO2 antibody was positively detected by IHC in:
human liver cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
IDO2 antibody was positively detected by FC in:
K562 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
ELISA
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Tested
Recommend
Human
Tested
Recommend
Recommend
Product Information
Product Name
Anti-IDO-2/IDO2 Antibody Picoband®
View all IDO2 Antibodies
View all IDO2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human IDO-2/IDO2 recombinant protein (Position: M1-R357).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated Molecular Weight
47.1 kDa
Background of IDO2
IDO2 (Indoleamine 2,3-dioxygenase 2), also called INDOLEAMINE 2,3-DIOXYGENASE-LIKE 1 or INDOL1, is an enzyme encoded by the INDOL1 gene which metabolizes tryptophan in the kynurenine pathway. By genomic sequence analysis, the INDOL1 gene is mapped on chromosome 8p12 just downstream of the INDO gene. And its exact cytogenetic location is 8p11.21. By database analysis using INDO as probe, followed by RT-PCR of total RNA from various tissues, IDO2 is cloned by human and mouse INDOL1. INDOL1 catabolizes tryptophan as determined by Kyn production, but unlike INDO, is inhibited by D-1-methyl-tryptophan (D-1MT) but not the L-1MT stereoisomer. The Gene Structure of the INDOL1 has 11 exons and spans 74 kb.
Cite This Product
Anti-IDO-2/IDO2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06002-2)
Protocols
Western Blot protocol for IDO2 antibody
Immunohistochemistry (IHC) protocol for IDO2 antibody
Flow Cytometry (FC) protocol for IDO2 antibody
Publications
Application:
Species:
Effect of Gestational Diabetes on Postpartum Depression-like Behavior in Rats and Its Mechanism
Published: 2022-03-14
Journal: Nutrients
Application: WB
Impact Factor: 6.71
Species: Rat
PMID: 35334886
Review
Have you used Anti-IDO-2/IDO2 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
$30 for a review with an image
Submit A ReviewProduct Promise
We promise Anti-IDO-2/IDO2 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A06002-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 29, 2026