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Product Info Summary
| SKU: | A04546-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MAGI2 Antibody
SKU/Catalog Number
A04546-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MAGI2 Antibody (Catalog # A04546-1). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MAGI2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04546-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human MAGI2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04546-1 is reactive to MAGI2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
Calculated molecular weight
158754 MW
Background of MAGI2
Seems to act as scaffold molecule at synaptic junctions by assembling neurotransmitter receptors and cell adhesion proteins. May play a role in regulating activin-mediated signaling in neuronal cells. Enhances the ability of PTEN to suppress AKT1 activation. Plays a role in nerve growth factor (NGF)-induced recruitment of RAPGEF2 to late endosomes and neurite outgrowth.
Florindo C.S., Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Devroe E., J. Biol. Chem. 279:24444-24451(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04546-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Positive Control
Validation Images & Assay Conditions
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Western blot analysis of extracts from HT-29 cells, using MAGI2 antibody A04546-1.
Protein Target Info & Infographic
Gene/Protein Information For MAGI2 (Source: Uniprot.org, NCBI)
Gene Name
MAGI2
Full Name
Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2
Weight
158754 MW
Superfamily
MAGUK family
Alternative Names
ACVRINP1; ACVRIP1; AIP1; AIP1AIP-1; ARIP1; atrophin 1 interacting protein 1; atrophin-1 interacting protein A; Atrophin-1-interacting protein 1; Atrophin-1-interacting protein A; KIAA0705activin receptor interacting protein 1; MAGI2; MAGI-2SSCAM; membrane associated guanylate kinase, WW and PDZ domain containing 2; Membrane-associated guanylate kinase inverted 2; membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2; SSCAM MAGI2 ACVRIP1, AIP-1, AIP1, ARIP1, MAGI-2, NPHS15, SSCAM membrane associated guanylate kinase, WW and PDZ domain containing 2 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2|activin receptor interacting protein 1|atrophin-1-interacting protein 1|atrophin-1-interacting protein A|membrane-associated guanylate kinase inverted 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAGI2, check out the MAGI2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAGI2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAGI2 Antibody (A04546-1)
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No publications found for A04546-1
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-MAGI2 Antibody
Question
Is a blocking peptide available for product anti-MAGI2 antibody (A04546-1)?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
We do provide the blocking peptide for product anti-MAGI2 antibody (A04546-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-11
Question
Will A04546-1 anti-MAGI2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
It shows on the product datasheet, A04546-1 anti-MAGI2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-25
Question
Here is the WB image, lot number and protocol we used for brain using anti-MAGI2 antibody A04546-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-28
Question
We are currently using anti-MAGI2 antibody A04546-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
The anti-MAGI2 antibody (A04546-1) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-27
Question
I see that the anti-MAGI2 antibody A04546-1 works with WB, what is the protocol used to produce the result images on the product page?
M. Williams
Verified customer
Asked: 2019-09-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-26
Question
I was wanting to use your anti-MAGI2 antibody for WB for rat brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2018-10-22
Answer
As indicated on the product datasheet, A04546-1 anti-MAGI2 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-22
Question
Does anti-MAGI2 antibody A04546-1 work on feline WB with metanephric glomerulus?
Verified Customer
Verified customer
Asked: 2018-08-16
Answer
Our lab technicians have not tested anti-MAGI2 antibody A04546-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-MAGI2 antibody A04546-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline metanephric glomerulus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-16
Question
I have a question about product A04546-1, anti-MAGI2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-07-13
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04546-1 anti-MAGI2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-13
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-MAGI2 antibody A04546-1. Let me know if you need anything else.
A. Jha
Verified customer
Asked: 2018-03-05
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-05
Question
Is this A04546-1 anti-MAGI2 antibody reactive to the isotypes of MAGI2?
Verified Customer
Verified customer
Asked: 2017-12-25
Answer
The immunogen of A04546-1 anti-MAGI2 antibody is Synthesized peptide derived from internal of human MAGI2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-25
Question
Would anti-MAGI2 antibody A04546-1 work for WB with brain?
Verified Customer
Verified customer
Asked: 2017-11-28
Answer
According to the expression profile of brain, MAGI2 is highly expressed in brain. So, it is likely that anti-MAGI2 antibody A04546-1 will work for WB with brain.
Boster Scientific Support
Answered: 2017-11-28
Question
Do you have a BSA free version of anti-MAGI2 antibody A04546-1 available?
J. Zhang
Verified customer
Asked: 2015-05-29
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-MAGI2 antibody A04546-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-05-29
Question
Our lab want to know about to test anti-MAGI2 antibody A04546-1 on rat brain for research purposes, then I may be interested in using anti-MAGI2 antibody A04546-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Johnson
Verified customer
Asked: 2013-08-27
Answer
The products we sell, including anti-MAGI2 antibody A04546-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-08-27
