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- Table of Contents
MBNL1 antibody (A02309-1) performs well in ELISA, IF, IHC, ICC, WB and shows reactivity with Human, Monkey, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IF analysis of MBNL1 using anti-MBNL1 antibody (A02309-1).
MBNL1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MBNL1 Antibody (A02309-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MBNL1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MBNL1 Antibody (A02309-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-MBNL1 Antibody Picoband®
MBNL1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey, Mouse, Rat | Rabbit | ELISA, IF, IHC, ICC, WB |
Tested Applications
MBNL1 antibody was positively detected by WB in:
human Jurkat whole cell, monkey COS-7 whole cell, human 293T whole cell, human Hela whole cell, human MOLT-4 whole cell, rat heart tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MBNL1 antibody was positively detected by IHC in:
human thyroid cancer tissue, human colorectal adenocarcinoma tissue, human laryngeal squamous cell carcinoma tissue, human liver cancer tissue, human ovarian serous adenocarcinoma tissue, mouse testis tissue, rat testis tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
MBNL1 antibody was positively detected by ICC/IF in:
A549 cell
MBNL1 antibody was positively detected by IF in:
human colon cancer tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Monkey, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Immunofluorescence
5μg/ml
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Monkey
Tested
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Rabbit
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-MBNL1 Antibody Picoband®
View all MBNL1 Antibodies
View all MBNL1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MBNL1 recombinant protein (Position: Q89-M388).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40 kDa
Calculated Molecular Weight
41.8 kDa
Background of MBNL1
Muscleblind-like (Drosophila), also known as MBNL1, is a protein that in humans is encoded by the MBNL1 gene. It is mapped to 3q25.1-q25.2. This gene encodes a member of the muscleblind protein family which was initially described in Drosophila melanogaster. The encoded protein is a C3H-type zinc finger protein that modulates alternative splicing of pre-mRNAs. Muscleblind proteins bind specifically to expanded dsCUG RNA but not to normal size CUG repeats and may thereby play a role in the pathophysiology of myotonic dystrophy. Mice lacking this gene exhibited muscle abnormalities and cataracts. Several alternatively spliced transcript variants have been described but the full-length natures of only some have been determined. The different isoforms are thought to have different binding specificities and/or splicing activities.
Cite This Product
Anti-MBNL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02309-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Mouse
99%
High Homology
Zebrafish
82%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for MBNL1 antibody
Immunohistochemistry (IHC) protocol for MBNL1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for MBNL1 antibody
Immunofluorescence (IF) protocol for MBNL1 antibody
Publications
Application:
Species:
Comparative Proteomic Analysis of tPVAT during Ang II Infusion
Published: 2021-12-02
Journal: Biomedicines
Application: IHC
Impact Factor: 6.08
Species: Mouse
PMID: 34944635
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Product Promise
We promise Anti-MBNL1 Antibody Picoband® performs excellently in relevant applications for ELISA, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A02309-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026