Anti-Ms CD11a Purified Itgal Monoclonal Antibody

Product Name

Anti-Ms CD11a Purified Itgal Monoclonal Antibody

View all ITGAL Antibodies

SKU/Catalog Number

M04466-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Ms CD11a Purified Itgal Monoclonal Antibody (Catalog# M04466-2). Tested in Flow Cytometry, IP, IHC-F application(s). This antibody reacts with Mouse.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Ms CD11a Purified Itgal Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04466-2)

Host

Rat

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

M17/4

Isotype

Rat IgG2a

Immunogen

C57BL/6 mouse splenic secondary cytotoxic T lymphocytes. The rat monoclonal antibody M17/4 reacts with an extracellular epitope of CD11a (alpha-subunit of murine LFA-1), a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.

Reactive Species

M04466-2 is reactive to ITGAL in Mouse

Observed Molecular Weight

19 kDa

Background of ITGAL

CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M04466-2 is guaranteed for Flow Cytometry, IP, IHC-F Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1 μg/ml.
Immunohistochemistry (frozen sections): Positive tissue: murine spleen or thymus, acetone fixation.

Validation Images & Assay Conditions

See full target information ITGAL

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