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Product Info Summary
| SKU: | M01911-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Mouse |
| Host: | Rat |
| Application: | Flow Cytometry, IHC-F, ICC |
Product info
Product Name
Anti-Ms CD49e Purified Itga5 Monoclonal Antibody
SKU/Catalog Number
M01911-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Ms CD49e Purified Itga5 Monoclonal Antibody (Catalog# M01911-1). Tested in ICC, Flow Cytometry, IHC-F application(s). This antibody reacts with Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Ms CD49e Purified Itga5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01911-1)
Host
Rat
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
5H10-27 (MFR5)
Isotype
Rat IgG2a kappa
Immunogen
C57BL/6 x A/J)F1 murine mast cell line. The rat monoclonal antibody 5H10-27 (MFR5) recognizes an extracellular epitope of murine CD49e, a 135 kDa protein serving as VLA-5 alpha chain, expressed on thymocytes, activated T cells, splenic B cells, and mast cells.
Reactive Species
M01911-1 is reactive to Itga5 in Mouse
Observed Molecular Weight
19 kDa
Calculated molecular weight
115.0 kDa
Background of Itga5
CD49e, the alpha 5 integrin, noncovalently associates with the beta 1integrin (CD29) to form VLA-5 integrin complex. CD49e itself is composed of two disulfide-linked chains of 135 kDa and 25 kDa. VLA-5 binds to RGD sequence of fibronectin, and to neural adhesion molecule L1. It is important in maintaining the integrity of endothelial monolayers, in monocyte migration into the extracellular tissues, and it also provides a co-stimulatory signal to T cells and enhances receptor and complement receptor-mediated phagocytosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01911-1 is guaranteed for Flow Cytometry, IHC-F, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Validation Images & Assay Conditions
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Separation of murine bone marrow cells stained using anti-mouse CD49e (5H10-27(MFR5)) purified antibody (concentration in sample 0.56 µg/ml, DAR APC) from murine bone marrow cells unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining).
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Flow cytometry surface staining pattern of murine bone marrow using anti-mouse CD49e (5H10-27(MFR5)) purified antibody (concentration in sample 0.56 µg/ml, DAR APC).
Specific Publications For Anti-Ms CD49e Purified Itga5 Monoclonal Antibody (M01911-1)
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