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Product Info Summary
| SKU: | M02236 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Mouse |
| Host: | Rat |
| Application: | Flow Cytometry, IP, IHC-P, IHC-F, ICC |
Product info
Product Name
Anti-Ms CD8a Purified Monoclonal Antibody
SKU/Catalog Number
M02236
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Ms CD8a Purified Monoclonal Antibody (Catalog# M02236). Tested in Flow Cytometry, IP, IHC-P, IHC-F, ICC application(s). This antibody reacts with Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Ms CD8a Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02236)
Host
Rat
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
53-6.7
Isotype
Rat IgG2a kappa
Immunogen
Murine spleen cells. The rat monoclonal antibody 53-6.7 recognizes an extracellular epitope of murine CD8a (32-34 kDa; alpha chain of the CD8 antigen).
Reactive Species
M02236 is reactive to CD8A in Mouse
Observed Molecular Weight
19 kDa
Calculated molecular weight
27.5 kDa
Background of CD8A
The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02236 is guaranteed for Flow Cytometry, IP, IHC-P, IHC-F, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1.5 μg/ml.
Immunohistochemistry (frozen sections): 1:1000; formaldehyde fixation is not recommended, acetone fixation is preferred.
Validation Images & Assay Conditions
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Separation of murine CD8a positive splenocytes (red-filled) from myeloid cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD8a (53-6.7) purified antibody (concentration in sample 3 µg/ml) DAR PE.
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Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD8a (53-6.7) purified antibody (concentration in sample 3 µg/ml) DAR PE.
Specific Publications For Anti-Ms CD8a Purified Monoclonal Antibody (M02236)
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