Anti-CD8 alpha/CD8A Antibody Picoband™

Boster Bio Anti-CD8 alpha/CD8A Antibody Picoband™ catalog # PB9249. Tested in IHC, WB applications. This antibody reacts with Human. Cited in 3 publication(s).

Product Info Summary

SKU: PB9249
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-CD8 alpha/CD8A Antibody Picoband™

See all CD8 products

SKU/Catalog Number

PB9249

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD8 alpha/CD8A Antibody Picoband™ catalog # PB9249. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD8 alpha/CD8A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9249)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human CD8 alpha recombinant protein (Position: S22-A187). Human CD8 alpha shares 49% and 47% amino acid (aa) sequence identity with mouse and rat CD8 alpha, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9249 is reactive to CD8A in Human

Applications

PB9249 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CD8A (Source: Uniprot.Org, NCBI)

Uniprot ID

P01732

Gene ID

925

Gene Name

CD8A

Full Name

T-cell surface glycoprotein CD8 alpha chain

Weight

25.729kDa

Alternative Names

53-6.7 CD8 Alpha; 53-6.7 CD8 Aplha; 53-6.7 CD8; 53-6.7 Clone; 53-6.7; CD8; CD8; CD8a molecule; CD8A; Cytotoxic T cell marker; Cytotoxic T lymphocyte marker; LEU2; p32; T cell marker CD8A CD8, Leu2, p32 CD8a molecule T-cell surface glycoprotein CD8 alpha chain|CD8 antigen, alpha polypeptide (p32)|Leu2 T-lymphocyte antigen|OKT8 T-cell antigen|T cell co-receptor|T-cell antigen Leu2|T-lymphocyte differentiation antigen T8/Leu-2|T8 T-cell antigen

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD8A, check out the CD8A Infographic

CD8A infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD8A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9249 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients

Xu Q, Chen Y, Zhao Wm, Huang Zy, Duan Xj, Tong Yy, Zhang Y, Li X, Chang Gb, Chen Gh. Mol Biol Rep. 2015 Feb;42(2):431-9. Doi: 10.1007/S11033-014-3784-3. Epub 2014 Oct 21. The Cd8?? Gene In Duck (Anatidae): Cloning, Characterization, And Expression...

Xu Y, Tan X, Wang D, Wang W, Li Y, Wu M, Chen S, Wu Y, Tan C. Int J Mol Med. 2015 Jun;35(6):1501-10. Doi: 10.3892/Ijmm.2015.2154. Epub 2015 Mar 24. Elevated Survivin Expression In Peripheral Blood Mononuclear Cells Is Central To Collateral Formati...

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16 Customer Q&As for Anti-CD8 alpha/CD8A Antibody Picoband™

Question

I see that the anti-CD8 alpha/CD8A antibody PB9249 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-03-19

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-19

Question

Would anti-CD8 alpha/CD8A antibody PB9249 work for WB with thymus?

Verified Customer

Verified customer

Asked: 2019-10-17

Answer

According to the expression profile of thymus, CD8A is highly expressed in thymus. So, it is likely that anti-CD8 alpha/CD8A antibody PB9249 will work for WB with thymus.

Boster Scientific Support

Answered: 2019-10-17

Question

We have seen staining in human pericardium. Do you have any suggestions? Is anti-CD8 alpha/CD8A antibody supposed to stain pericardium positively?

Verified Customer

Verified customer

Asked: 2019-10-04

Answer

From what I have seen in literature pericardium does express CD8A. From what I have seen in Uniprot.org, CD8A is expressed in thymus, pericardium, among other tissues. Regarding which tissues have CD8A expression, here are a few articles citing expression in various tissues:
Pericardium, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-10-04

Question

My team were satisfied with the WB result of your anti-CD8 alpha/CD8A antibody. However we have been able to see positive staining in pericardium isoform 2: secreted using this antibody. Is that expected? Could you tell me where is CD8A supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-09-26

Answer

From what I have seen in literature, pericardium does express CD8A. Generally CD8A expresses in isoform 1: cell membrane, isoform 2: secreted. Regarding which tissues have CD8A expression, here are a few articles citing expression in various tissues:
Pericardium, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-09-26

Question

I was wanting to use using your anti-CD8 alpha/CD8A antibody for antigen processing and presentation studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-09-18

Answer

Thanks for your inquiry. This PB9249 anti-CD8 alpha/CD8A antibody is validated on human tonsil tissue, jurkat whole cell lysate, raji whole cell lysate, a549 whole cell lysate, colo320 whole cell lysate, hela whole cell lysate. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-09-18

Question

Is there a BSA free version of anti-CD8 alpha/CD8A antibody PB9249 available?

Verified Customer

Verified customer

Asked: 2019-09-13

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD8 alpha/CD8A antibody PB9249 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-13

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymus using anti-CD8 alpha/CD8A antibody PB9249. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-05-13

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-13

Question

Does PB9249 anti-CD8 alpha/CD8A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-12-31

Answer

You can see on the product datasheet, PB9249 anti-CD8 alpha/CD8A antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-12-31

Question

Is a blocking peptide available for product anti-CD8 alpha/CD8A antibody (PB9249)?

Verified Customer

Verified customer

Asked: 2017-08-28

Answer

We do provide the blocking peptide for product anti-CD8 alpha/CD8A antibody (PB9249). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-08-28

Question

Here is the WB image, lot number and protocol we used for thymus using anti-CD8 alpha/CD8A antibody PB9249. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-05-19

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-05-19

Question

Is this PB9249 anti-CD8 alpha/CD8A antibody reactive to the isotypes of CD8A?

K. Jha

Verified customer

Asked: 2017-05-12

Answer

The immunogen of PB9249 anti-CD8 alpha/CD8A antibody is E.coli-derived human CD8 alpha recombinant protein (Position: S22-A187). Human CD8 alpha shares 49% and 47% amino acid (aa) sequence identity with mouse and rat CD8 alpha, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-05-12

Question

We purchased anti-CD8 alpha/CD8A antibody for WB on pericardium in a previous project. I am using human, and We want to use the antibody for IHC next. My lab would like examining pericardium as well as thymus in our next experiment. Could give a recommendation on which antibody would work the best for IHC?

L. Yang

Verified customer

Asked: 2017-05-08

Answer

I took a look at the website and datasheets of our anti-CD8 alpha/CD8A antibody and it seems that PB9249 has been tested on human in both WB and IHC. Thus PB9249 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-05-08

Question

My question regarding product PB9249, anti-CD8 alpha/CD8A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

K. Moore

Verified customer

Asked: 2014-10-27

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9249 anti-CD8 alpha/CD8A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-10-27

Question

We want to test anti-CD8 alpha/CD8A antibody PB9249 on human thymus for research purposes, then I may be interested in using anti-CD8 alpha/CD8A antibody PB9249 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

B. Edwards

Verified customer

Asked: 2014-01-10

Answer

The products we sell, including anti-CD8 alpha/CD8A antibody PB9249, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2014-01-10

Question

We are currently using anti-CD8 alpha/CD8A antibody PB9249 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?

R. Krishna

Verified customer

Asked: 2013-06-18

Answer

The anti-CD8 alpha/CD8A antibody (PB9249) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-06-18

Question

I was wanting to use your anti-CD8 alpha/CD8A antibody for WB for human thymus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human thymus identification?

F. Li

Verified customer

Asked: 2013-02-15

Answer

You can see on the product datasheet, PB9249 anti-CD8 alpha/CD8A antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human thymus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-02-15

Conjugation

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PB9249
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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