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Product Info Summary
| SKU: | A02236-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-CD8 alpha/Cd8a Antibody Picoband®
SKU/Catalog Number
A02236-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD8 alpha/Cd8a Antibody Picoband® catalog # A02236-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD8 alpha/Cd8a Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02236-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat CD8 alpha/Cd8a recombinant protein (Position: Q27-Q114).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02236-2 is reactive to Cd8a in Rat
Observed Molecular Weight
26-35 kDa
Calculated molecular weight
26.2 kDa
Background of Cd8a
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02236-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat thymus issue, rat thymus issue
IHC: rat spleen tissue, rat thymus tissue
Validation Images & Assay Conditions
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IHC analysis of CD8A using anti-CD8A antibody (A02236-2). CD8A was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD8A Antibody (A02236-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of CD8A using anti-CD8A antibody (A02236-2).
CD8A was detected in paraffin-embedded section of rat thymus tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD8A Antibody (A02236-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Western blot analysis of CD8A using anti-CD8A antibody (A02236-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus issue lysates,
Lane 2: rat thymus issue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD8A antigen affinity purified polyclonal antibody (Catalog # A02236-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD8A at approximately 26-35KD. The expected band size for CD8A is at 26KD.
Specific Publications For Anti-CD8 alpha/Cd8a Antibody Picoband® (A02236-2)
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4 Customer Q&As for Anti-CD8 alpha/Cd8a Antibody Picoband®
Question
We have seen staining in rat pericardium. Are there any suggestions? Is anti-CD8 alpha/Cd8a antibody supposed to stain pericardium positively?
M. Miller
Verified customer
Asked: 2020-02-28
Answer
From literature pericardium does express CD8A. From Uniprot.org, CD8A is expressed in thymus, pericardium, among other tissues. Regarding which tissues have CD8A expression, here are a few articles citing expression in various tissues:
Pericardium, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-02-28
Question
My team were content with the WB result of your anti-CD8 alpha/Cd8a antibody. However we have seen positive staining in pericardium isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is CD8A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
According to literature, pericardium does express CD8A. Generally CD8A expresses in isoform 1: cell membrane, isoform 2: secreted. Regarding which tissues have CD8A expression, here are a few articles citing expression in various tissues:
Pericardium, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-08-15
Question
Our lab used your anti-CD8 alpha/Cd8a antibody for ELISA on pericardium a few months ago. I am using rat, and We want to use the antibody for WB next. My lab would like examining pericardium as well as thymus in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
A. Jha
Verified customer
Asked: 2018-03-05
Answer
I viewed the website and datasheets of our anti-CD8 alpha/Cd8a antibody and I see that A02236-2 has been validated on rat in both ELISA and WB. Thus A02236-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-03-05
Question
We are currently using anti-CD8 alpha/Cd8a antibody A02236-2 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says rat. Is it likely that the antibody can work on canine tissues as well?
J. Parker
Verified customer
Asked: 2016-01-19
Answer
The anti-CD8 alpha/Cd8a antibody (A02236-2) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-01-19
