Anti-CD8 alpha/Cd8a Antibody Picoband™

CD8 antibody

Boster Bio Anti-CD8 alpha/Cd8a Antibody Picoband™ catalog # A02236-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A02236-2
Size: 100 μg/vial
Reactive Species: Rat
Host: Rabbit
Application: ELISA, IHC, WB

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Product Name

Anti-CD8 alpha/Cd8a Antibody Picoband™

View all CD8 Antibodies

SKU/Catalog Number

A02236-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD8 alpha/Cd8a Antibody Picoband™ catalog # A02236-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD8 alpha/Cd8a Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02236-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived rat CD8 alpha/Cd8a recombinant protein (Position: Q27-Q114).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02236-2 is reactive to Cd8a in Rat

Applications

A02236-2 is guaranteed for ELISA, IHC, WB Boster Guarantee

Background of CD8

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For Cd8a (Source: Uniprot.org, NCBI)

Uniprot ID

P07725

Gene ID

24930

Gene Name

Cd8a

Full Name

T-cell surface glycoprotein CD8 alpha chain

Weight

26.196kDa

Alternative Names

53-6.7 CD8 Alpha; 53-6.7 CD8 Aplha; 53-6.7 CD8; 53-6.7 Clone; 53-6.7; CD8; CD8; CD8a molecule; CD8A; Cytotoxic T cell marker; Cytotoxic T lymphocyte marker; LEU2; p32; T cell marker Cd8a|CD8a molecule|T-cell surface glycoprotein CD8 alpha chain|CD8 antigen 32 kDa chain|CD8 antigen, alpha-chain|OX-8 membrane antigen

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Cd8a, check out the Cd8a Infographic

Cd8a infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Cd8a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact sup[email protected]

A02236-2 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Xu Y, Tan X, Wang D, Wang W, Li Y, Wu M, Chen S, Wu Y, Tan C. Int J Mol Med. 2015 Jun;35(6):1501-10. Doi: 10.3892/Ijmm.2015.2154. Epub 2015 Mar 24. Elevated Survivin Expression In Peripheral Blood Mononuclear Cells Is Central To Collateral Formati...

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4 Customer Q&As for Anti-CD8 alpha/Cd8a Antibody Picoband™

Question

We have seen staining in rat pericardium. Are there any suggestions? Is anti-CD8 alpha/Cd8a antibody supposed to stain pericardium positively?

M. Miller

Verified customer

Asked: 2020-02-28

Answer

From literature pericardium does express CD8A. From Uniprot.org, CD8A is expressed in thymus, pericardium, among other tissues. Regarding which tissues have CD8A expression, here are a few articles citing expression in various tissues:
Pericardium, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-02-28

Question

My team were content with the WB result of your anti-CD8 alpha/Cd8a antibody. However we have seen positive staining in pericardium isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is CD8A supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

According to literature, pericardium does express CD8A. Generally CD8A expresses in isoform 1: cell membrane, isoform 2: secreted. Regarding which tissues have CD8A expression, here are a few articles citing expression in various tissues:
Pericardium, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-08-15

Question

Our lab used your anti-CD8 alpha/Cd8a antibody for ELISA on pericardium a few months ago. I am using rat, and We want to use the antibody for WB next. My lab would like examining pericardium as well as thymus in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

A. Jha

Verified customer

Asked: 2018-03-05

Answer

I viewed the website and datasheets of our anti-CD8 alpha/Cd8a antibody and I see that A02236-2 has been validated on rat in both ELISA and WB. Thus A02236-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-03-05

Question

We are currently using anti-CD8 alpha/Cd8a antibody A02236-2 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says rat. Is it likely that the antibody can work on canine tissues as well?

J. Parker

Verified customer

Asked: 2016-01-19

Answer

The anti-CD8 alpha/Cd8a antibody (A02236-2) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-01-19

Size

Conjugation

Total: $315

SKU:A02236-2

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A02236-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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