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Product Info Summary
| SKU: | M30404 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Mouse |
| Host: | Rat |
| Application: | Flow Cytometry, IP, IHC-P, IHC-F, WB, Functional Assay |
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Product info
Product Name
Anti-Ms Ly6G Purified Low Endotoxin Monoclonal Antibody
SKU/Catalog Number
M30404
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Ms Ly6G Purified Low Endotoxin Monoclonal Antibody (Catalog# M30404). Tested in Flow Cytometry, IP, WB, IHC-P, IHC-F, Functional Assay application(s). This antibody reacts with Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Ms Ly6G Purified Low Endotoxin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30404)
Host
Rat
Contents
Phosphate buffered saline (PBS), pH 7.4
Clonality
Monoclonal
Clone Number
RB6-8C5
Isotype
Rat IgG2b
Immunogen
Murine granulocytes. The rat monoclonal antibody RB6-8C5 detects Ly6G component of Gr-1 antigen, a commonly used surface marker of neutrophils.
Reactive Species
M30404 is reactive to LY6G in Mouse
Observed Molecular Weight
19 kDa
Background of LY6G
Ly6G is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. Ly6G is a good marker for detection of peripheral neutrophils. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. Physiological role of Ly6G remains still unclear. Its treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M30404 is guaranteed for Flow Cytometry, IP, IHC-P, IHC-F, WB, Functional Assay Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Functional application: Neutrophil depletion.
Immunoprecipitation: 1-2 μg / 100-500 μg of protein.
Western blotting: 1 μg/ml.
Flow cytometry: 1-4 µg/ml
Validation Images & Assay Conditions
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Separation of murine Ly6G positive cells (red-filled) from Ly6G negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse Ly6G (RB6-8C5) purified antibody (low endotoxin, concentration in sample 9 µg/ml) DAR PE.
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Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse Ly6G (RB6-8C5) purified antibody (low endotoxin, concentration in sample 9 µg/ml) DAR PE.
Specific Publications For Anti-Ms Ly6G Purified Low Endotoxin Monoclonal Antibody (M30404)
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