Anti-Ms Ly6G Purified Low Endotoxin Monoclonal Antibody

Product Name

Anti-Ms Ly6G Purified Low Endotoxin Monoclonal Antibody

View all Ly6g Antibodies

SKU/Catalog Number

M30404

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Ms Ly6G Purified Low Endotoxin Monoclonal Antibody (Catalog# M30404). Tested in Flow Cytometry, IP, WB, IHC-P, IHC-F, Functional Assay application(s). This antibody reacts with Mouse.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Ms Ly6G Purified Low Endotoxin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30404)

Host

Rat

Contents

Phosphate buffered saline (PBS), pH 7.4

Clonality

Monoclonal

Clone Number

RB6-8C5

Isotype

Rat IgG2b

Immunogen

Murine granulocytes. The rat monoclonal antibody RB6-8C5 detects Ly6G component of Gr-1 antigen, a commonly used surface marker of neutrophils.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M30404 is reactive to Ly6g in Mouse

Applications

M30404 is guaranteed for Flow Cytometry, IP, IHC-P, IHC-F, WB, Functional Assay Boster Guarantee

Observed Molecular Weight

19 kDa

Calculated molecular weight

14.324kDa

Background of Ly6g

Ly6G is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. Ly6G is a good marker for detection of peripheral neutrophils. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. Physiological role of Ly6G remains still unclear. Its treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Functional application: Neutrophil depletion.
Immunoprecipitation: 1-2 μg / 100-500 μg of protein.
Western blotting: 1 μg/ml.
Flow cytometry: 1-4 µg/ml

Validation Images & Assay Conditions

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Figure 1. Flow Cytometry validation of Ly6G using Anti-Ms Ly6G Purified Low Endotoxin Monoclonal Antibody (M30404).

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse Ly6G (RB6-8C5) purified antibody (low endotoxin

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Figure 2. Flow Cytometry validation of Ly6G using Anti-Ms Ly6G Purified Low Endotoxin Monoclonal Antibody (M30404).

Separation of murine Ly6G positive cells (red-filled) from Ly6G negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse Ly6G (RB6-8C5) purified antibody (low endotoxin

Gene/Protein Information For Ly6g (Source: Uniprot.org, NCBI)

Alternative Names

Nothing Found Ly6g|Gr, Gr-, Gr-1, Gr1, Ly-6, Ly-6G|lymphocyte antigen 6 complex, locus G|lymphocyte antigen 6G|ly-6G.1

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In this infographic, you will see the following information for Ly6g: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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