SDS-PAGE Protein Loading Buffer 5X (Reducing)

SDS-PAGE Protein Loading Buffer 5X (Reducing) Cited in 10 publication(s).

Product Info Summary

SKU: AR1112
Size: From 3mL to 20mL

General Description

Boster’s SDS PAGE Sample Buffer 5X (Reducing) is the most commonly used sample buffer for Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) of denatured proteins in the Laemmli SDS-PAGE system.

This buffer is very important in the preparation of protein samples and loading them onto a gel. Boster protein loading buffer is a 5X reducing hence it requires less sample dilution but yet accommodating more proteins load in a well.

Overview

Product Name SDS-PAGE Protein Loading Buffer 5X (Reducing)
Form Liquid
Contents 10% SDS, 500mM DTT, 50% Glycerol, 250mM Tris-HCL and 0.5% bromophenol blue dye, PH6.8.
Equivalent GeneCopoeia (Product No. P013)
Storage Upon receipt store at -20°C. SDS-PAGE Protein Loading Buffer 5X (Reducing) is stable for one year. Product is shipped on ice.

Assay Principle

Using bromophenol blue dye, SDS-PAGE Protein Loading Buffer is a ready-to-use 5X solution. It contains 10% SDS, 500Mm DTT, 50% Glycerol, 500mM Tris-HCL and 0.05% bromophenol blue dye. It can be used for SDS-PAGE protein loading of conventional proteins. It is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. It is sufficient to prepare 15 mL protein samples. The whole sample preparation process can be finished within 5 minutes.

Remember to avoid common mistakes that may occur such as underloading or overloading of proteins. This can be avoided by considering the amount of protein available using protein assay. Loading less protein may lead to poor detection of the bands while on the other hand overloading the protein would lead to the formation of streaks or even smears due to protein aggregation.

It is always advisable to run you gel electrophoresis markers together with the protein samples so that you can observe accurate protein sizes and also act as control

Assay Protocol

1. Equilibrate SDS-PAGE Protein Loading Buffer 5X to room temperature or thaw Loading Buffer in a water bath no higher than 30°C.

2. Mix one volume of SDS-PAGE Protein Loading Buffer 5X with four volume of protein sample (i.e. add 4mL protein sample into 1 mL Loading Buffer).

3. Boil sample for 3-5 min.
Note: If semitransparent viscous substance remains after boiling, boil sample for another 5-10 min or add 1X SDS-PAGE Protein Loading Buffer which is diluted from 5X SDS-PAGE Protein Loading Buffer to the sample and then boil for another 3-5 min.

4. Allow sample to cool to room temperature.

5. Load sample into the wells of the SDS-PAGE gel and begin electrophoresis.

6. Stop electrophoresis when bromophenol blue dye front reaches to the bottom of the gel.

General Notes

1. SDS-PAGE Protein Loading Buffer 5X contains DTT which has a slightly irritating odor.

2. SDS-PAGE Protein Loading Buffer 5X contains DTT which has a slightly irritating odor.

3. Please wear the lab coat and disposable gloves and operate in a laboratory hood.

Validation Images & Assay Conditions

AR1112 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Potential anti-vitiligo properties of cynarine extracted from Vernonia anthelmintica (L.) Willd

HMGB1 Mediates Autophagy Dysfunction via Perturbing Beclin1-Vps34 Complex in Dopaminergic Cell Model

Constant or fluctuating hyperglycemias increases cytomembrane stiffness of human umbilical vein endothelial cells in culture: roles of cytoskeletal rearrangement and nitric oxide synthesis

Interleukin 6 secretion from alternatively activated macrophages promotes the migration of endometriotic epithelial cells

The role of Sox9 in collagen hydrogel-mediated chondrogenic differentiation of adult mesenchymal stem cells (MSCs)

Intrastriatal injection of ionomycin profoundly changes motor response to l-DOPA and its underlying molecular mechanisms

Induction of fiber-like aggregation and gelation of collagen by ultraviolet irradiation at low temperature

Effect of ultra-high pressure on molecular structure and properties of bullfrog skin collagen

tmbim4 protects against triclocarban-induced embryonic toxicity in zebrafish by regulating autophagy and apoptosis

Prolonged skin grafts survival time by IFN-γ in allogeneic skin transplantation model during acute rejection through IFN-γ/STAT3/IDO pathway in epidermal layer

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2 Customer Q&As for SDS-PAGE Protein Loading Buffer 5X (Reducing)

Question

Is AR1112 compatible with tris glycine?

Verified customer

Asked: 2021-02-26

Answer

Yes, the SDS-PAGE Protein Loading Buffer 5X (Reducing) (AR1112) is compatible with tris glycine.

Boster Scientific Support

Answered: 2021-03-01

Question

Can you clarify in your SDS recipe for AR1112 Buffer having the content that says the concentration of Bromophenol Blue dye used was 0.5%, whereas in the Assay Principle it says 0.05% of the same dye?

Verified customer

Asked: 2019-10-17

Answer

Our lab technicians confirmed that SDS-PAGE Protein Loading Buffer 5X (Reducing) AR1112 contains 0.5% Bromophenol Blue dye.

Boster Scientific Support

Answered: 2019-10-17

Size

Total: $10

SKU:AR1112

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