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Product Info Summary
| SKU: | A05497-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-NEIL3/Nei3 Antibody
SKU/Catalog Number
A05497-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-NEIL3/Nei3 Antibody catalog # A05497-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NEIL3/Nei3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05497-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human NEIL3. AA range:491-540
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05497-1 is reactive to NEIL3 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
67.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05497-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Western Blot analysis of various cells using NEIL3 Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysates from HeLa, HepG2, HUVEC, and COLO cells, using NEIL3 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-NEIL3/Nei3 Antibody (A05497-1)
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3 Customer Q&As for Anti-NEIL3/Nei3 Antibody
Question
We are currently using anti-NEIL3/Nei3 antibody A05497-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
The anti-NEIL3/Nei3 antibody (A05497-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-29
Question
Is there a BSA free version of anti-NEIL3/Nei3 antibody A05497-1 available?
Verified Customer
Verified customer
Asked: 2018-12-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NEIL3/Nei3 antibody A05497-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-12-26
Question
I am interested in to test anti-NEIL3/Nei3 antibody A05497-1 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-NEIL3/Nei3 antibody A05497-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Banerjee
Verified customer
Asked: 2013-08-30
Answer
The products we sell, including anti-NEIL3/Nei3 antibody A05497-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-08-30
