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Product Info Summary
| SKU: | A05845 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-p70 S6 kinase beta RPS6KB2 Antibody
SKU/Catalog Number
A05845
Size
100μl
Form
Liquid
Description
Boster Bio Anti-p70 S6 kinase beta RPS6KB2 Antibody catalog # A05845. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p70 S6 kinase beta RPS6KB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05845)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase beta. AA range:389-438
Reactive Species
A05845 is reactive to RPS6KB2 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
53.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05845 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Western Blot analysis of various cells using p70 S6 kinase β Polyclonal Antibody
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Western Blot analysis of 293 cells using p70 S6 kinase β Polyclonal Antibody
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Western blot analysis of lysates from 293 cells, using p70 S6 Kinase beta Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HUVECcells using p70 S6 Kinase β antibody.
Specific Publications For Anti-p70 S6 kinase beta RPS6KB2 Antibody (A05845)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-p70 S6 kinase beta RPS6KB2 Antibody
Question
We are currently using anti-p70 S6 kinase beta antibody A05845 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
The anti-p70 S6 kinase beta antibody (A05845) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-29
Question
We are interested in to test anti-p70 S6 kinase beta antibody A05845 on human leukemic t-cell for research purposes, then I may be interested in using anti-p70 S6 kinase beta antibody A05845 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-11-17
Answer
The products we sell, including anti-p70 S6 kinase beta antibody A05845, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-17
Question
Does anti-p70 S6 kinase beta antibody A05845 work for WB with leukemic t-cell?
C. Collins
Verified customer
Asked: 2017-07-06
Answer
According to the expression profile of leukemic t-cell, RPS6KB2 is highly expressed in leukemic t-cell. So, it is likely that anti-p70 S6 kinase beta antibody A05845 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2017-07-06
