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PARP16 antibody (A10545-1) performs well in ELISA, Flow Cytometry, IHC and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of HL-60 cells using anti-PARP16 antibody (A10545-1).
Overlay histogram showing HL-60 cells stained with A10545-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PARP16 Antibody (A10545-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HL-60 cells stained with A10545-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PARP16 Antibody (A10545-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-PARP16 Antibody
PARP16 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IHC |
Tested Applications
PARP16 antibody was positively detected by IHC in:
human breast cancer tissue, human thyroid cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
PARP16 antibody was positively detected by IF in:
human thyroid cancer tissue
PARP16 antibody was positively detected by FC in:
HL-60 cell
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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IHC
IF
ELISA
Human
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-PARP16 Antibody
View all PARP16 Antibodies
View all PARP16 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PARP16 recombinant protein (Position: A9-Y254). Human PARP16 shares 92.3% and 90.2% amino acid (aa) sequence identity with mouse and rat PARP16, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Calculated Molecular Weight
36.4 kDa
Background of PARP16
Enables kinase binding activity; pentosyltransferase activity; and protein serine/threonine kinase activator activity. Involved in IRE1-mediated unfolded protein response; negative regulation of cytoplasmic translation; and protein auto-ADP-ribosylation. Located in endoplasmic reticulum tubular network and nuclear envelope.
Cite This Product
Anti-PARP16 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10545-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Rabbit
98%
High Homology
Sheep
93%
High Homology
Cow
93%
High Homology
Mouse
92%
High Homology
Dog
92%
High Homology
Rat
90%
High Homology
Zebrafish
63%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for PARP16 antibody
Immunofluorescence (IF) protocol for PARP16 antibody
Flow Cytometry (FC) protocol for PARP16 antibody
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Product Promise
We promise Anti-PARP16 Antibody performs excellently in relevant applications for ELISA, Flow Cytometry, IHC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A10545-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026