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Product Info Summary
| SKU: | P01890 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Phospho-PKC Gamma/PRKCG (T514) Antibody
SKU/Catalog Number
P01890
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Phospho-PKC Gamma/PRKCG (T514) Antibody catalog # P01890. Tested in WB, ICC, IF applications. This antibody reacts with Human, Rat.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-Phospho-PKC Gamma/PRKCG (T514) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01890)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
A synthesized peptide derived from human PKC γ around the phosphorylation site of Thr514.
Reactive Species
P01890 is reactive to PRKCG in Human, Rat
Calculated molecular weight
78.4 kDa
Background of PRKCG
The gamma isotype of protein kinase C (PKC gamma) is a member of the classical PKC (cPKC) subfamily which is activated by Ca(2+) and diacylglycerol in the presence of phosphatidylserine. Physiologically, PKC gamma is activated by a mechanism coupled with receptor-mediated breakdown of inositol phospholipid as other cPKC isotypes such as PKC alpha and PKC beta. PKC gamma is expressed solely in the brain and spinal cord and its localization is restricted to neurons, while PKC alpha and PKC beta are expressed in many tissues in addition to the brain. Within the brain, PKC gamma is the most abundant in the cerebellum, hippocampus and cerebral cortex, where the existence of neuronal plasticity has been demonstrated. PKC gamma gene is mutated in spinocerebellar ataxia type 14 (SCA14). Verbeek et al. (2005) point out the specific alterations in mutant PKC gamma function that could lead to the selective neuronal degeneration of SCA14.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01890 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunocytochemistry/Immunofluorescence, 1:50-400
Validation Images & Assay Conditions
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Specific Publications For Anti-Phospho-PKC Gamma/PRKCG (T514) Antibody (P01890)
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