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Product Info Summary
| SKU: | A03479 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Protein Wnt-10a Wnt10a Antibody
SKU/Catalog Number
A03479
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein Wnt-10a Wnt10a Antibody (Catalog # A03479). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Wnt10a antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein Wnt-10a Wnt10a Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03479)
Host
Rabbit
Contents
Wnt10a Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Wnt10a antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Wnt10a. The immunogen is located within amino acids 300 - 350 of Wnt10a.
Cross-reactivity
This Wnt10a antibody is predicted to not cross-react with Wnt10b.
Reactive Species
A03479 is reactive to WNT10A in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
46.4 kDa
Background of WNT10A
Wnt10a is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Elevated levels of Wnt10a have been detected in several human tumors and cancer cell lines including colorectal cancer and chronic lymphocytic leukemia and it has been suggested that uncontrolled Wnt signaling may contribute to the defect in apoptosis that characterizes these malignancies. Recently, a mutation in Wnt10a was found to be associated with odonto-onycho-dermal dysplasia, an autosomal recessive ectodermal dysplasia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03479 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Wnt10a antibody can be used for detection of Wnt10a by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Wnt10a in RAW264.7 cell lysate with Wnt10a antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of Wnt10a in human skeletal muscle tissue with Wnt10a antibody at 10 μg/mL.
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Immunofluorescence of Wnt10a in Human Skeletal Muscle tissue with Wnt10a antibody at 20 μg/mL.
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Immunohistochemistry of Wnt10a in mouse skeletal muscle tissue with Wnt10a antibody at 5 μg/ml.
Specific Publications For Anti-Protein Wnt-10a Wnt10a Antibody (A03479)
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