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Product Info Summary
| SKU: | A30032 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Survivin birc5.1-a Antibody
SKU/Catalog Number
A30032
Size
50 µl
Form
Liquid
Description
Boster Bio Anti-Survivin birc5.1-a Antibody (Catalog# A30032). Tested in IP, IHC, WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-Survivin birc5.1-a Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30032)
Host
Rabbit
Contents
50 μl sera
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A Full-length recombinant protein of human Survivin
Reactive Species
A30032 is reactive to BIRC5 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
16.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30032 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000-1:2000
IHC (paraffin): 1:1000-1:5000
IHC (frozen): Users should optimize
IP: 1:50-1:200
Validation Images & Assay Conditions
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Immunohistochemical analysis of Survivin in a formalin-fixed, paraffin-embedded human brain tissue microarray using primary antibody dilution ratio of 1:2000. A. Differential expression of Survivin in brain tumors from six different patients. A1-A3, high magnification from A. A1, gemistocytic astrocytoma. A2, medulloblastoma. A3, choroid plexus papilloma. Hematoxylin-eosin counterstain.
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Immunohistochemical analysis of Survivin in formalin-fixed, paraffin-embedded human prostate and breast cancer using primary antibody dilution ratio of 1:2000. A and A1, prostate cancer at low and high magnification, respectively. B and B1, breast cancer. B1 is a high magnification of an area of metastasis. Hematoxylin-eosin counterstain.
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Western blot analysis of Survivin using primary antibody dilution ratio of 1:2000. Survivin was detected in the two tumor cell lines, Jurkat (T cell lymphoma) and RS11846 (non-Hodgkin's lymphoma), but not in lysates from normal tissues. Survivin RP, full-length recombiant human survivin protein was used as a positive control.
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Agarose gel of some apoptosis-related genes in S. frugiperda PCR amplified with the specific primers shown in Supplement Table . Lanes: M, Marker 2000, Lane 1–15, Sf-Caspase-1 , Sf-Caspase-2 , Sf-AIF , Sf-BI , Sf-IBM1 , Sf-Grim-19 , Sf-Survivin , Sf-IAP , Sf-Ras , Sf-Cyt C , Sf-Traf 6 , Sf-Pcdp 5 , Sf-Rptor , Sf-Pkar 1 , and Sf-Buffy .
Index in PubMed under a CC BY license. PMID: 29038528
Specific Publications For Anti-Survivin birc5.1-a Antibody (A30032)
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