Anti-TAF1/Kat4 Antibody

Product Name

Anti-TAF1/Kat4 Antibody

View all TAF1 Antibodies

SKU/Catalog Number

A02151

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-TAF1/Kat4 Antibody (Catalog # A02151). Tested in ChIP, ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-TAF1/Kat4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02151)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human TAF1.

Reactive Species

A02151 is reactive to TAF1 in Human, Mouse

Observed Molecular Weight

42 kDa

Calculated molecular weight

212.7 kDa

Background of TAF1

Anti-TAF1 antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Transcription Initiation Factor TFIID Subunit 1 (TAF1) is the largest component and core scaffold of the TFIID basal transcription factor complex, which also includes TATA-binding protein (TBP) and a variety of TBP-associated factors. TFIID nucleates the formation of transcription pre-initiation complexes and plays a key role in the regulation of gene expression by RNA polymerase II. TAF1 possesses DNA-binding activity and also contains novel N- and C-terminal Ser/Thr kinase domains which can auto-phosphorylate or trans-phosphorylate other transcription factors. For example, TAF1 interacts with the C-terminus of TP53 and phosphorylates the T55 residue, leading to MDM2-mediated degradation of TP53.  TAF1 also catalyzes Ser phosphorylation of general transcription factor IIA (GTF2A1) and IIF (GTF2F1). The retinoblastoma tumor suppressor protein, RB1, interacts with the N-terminal domain of TAF1 and inhibits its intrinsic kinase activity. TAF1 is essential for progression through the G1 phase of the cell cycle and has been reported to be indispensable for expression of 18% of mammalian genes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02151 is guaranteed for ChIP, ELISA, IP, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:150,000 - 1:300,000
ChIP: User optimized
IHC: 10µg/ml
IP: User optimized
WB: 1:100 - 1:2,000
This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, ChIP, and western blotting. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 213 kDa in size corresponding to TAF1 protein by western blotting in the appropriate cell lysate or extract.

Validation Images & Assay Conditions

See full target information TAF1

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