|Validated Species:||Chicken, Human, Mouse, Rat|
Data & Images
|Product Name||Anti-Talin Antibody (Monoclonal, 8d4)|
|Description||Mouse IgG monoclonal antibody for Talin, talin 1; talin 2 (TLN1; TLN2) detection. Tested with WB in Human;mouse;rat;chicken. No cross reactivity with other proteins.|
|Cite This Product||Anti-Talin Antibody (Monoclonal, 8d4) 8d4 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1092)|
|Replacement Item||This antibody may replace the following items: sc-20936|sc-56917|sc-57026|sc-377530|sc-53335|sc-53334|sc-21089-R from Santa Cruz Biotechnology.|
|Validated Species||Chicken, Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||Purified chicken gizzard talin.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Talin-1; Talin-2|
|Molecular Weight||271684 MW|
|Protein Function||TLN1: Probably involved in connections of major cytoskeletalstructures to the plasma membrane. High molecular weightcytoskeletal protein concentrated at regions of cell-substratumcontact and, in lymphocytes, at cell-cell contacts (Bysimilarity).
TLN2: As a major component of focal adhesion plaques thatlinks integrin to the actin cytoskeleton, may play an importantrole in cell adhesion. Recruits PIP5K1C to focal adhesion plaquesand strongly activates its kinase activity (By similarity).
|Sequence Similarities||Contains 1 FERM domain.|
|Subcellular Localization||TLN1: Cell projection, ruffle membrane Cytoplasm, cytoskeleton Cell surface Cell junction, focaladhesion Note: Colocalizes with LAYN at the membraneruffles. Localized preferentially in focal adhesions thanfibrillar adhesions (By similarity).
Tln1: Cell projection, ruffle membrane Cytoplasm, cytoskeleton Cell surface
TLN2: Cell junction, focal adhesion. Celljunction, synapse. Cell membrane; Peripheral membrane protein;Cytoplasmic side. Cytoplasm, cytoskeleton. Note: Focal adhesionplaques and synapses.
Tln2: Cell junction, focal adhesion Cell junction, synapse Cell membrane Cytoplasm, cytoskeleton Note: Focaladhesion plaques and synapses.
|Alternative Names||Protein Tln2 ;Tln2 ;|
|Research Areas||signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|talin / moesin||
Background for Talin-1; Talin-2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Talin Antibody (Monoclonal, 8d4) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 2-4μg/ml, Human, mouse, rat, chicken|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.