SKU MA1092
Size 100μg/vial
Reactivity Chicken, Human, Mouse, Rat
Clonality Monoclonal
Clone Number 8d4
Host Mouse
Ig Isotype Mouse IgG1
Applications WB

Overview

Product Name Anti-Talin Antibody (Monoclonal, 8d4)
SKU/Catalog Number MA1092
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Talin, talin 1; talin 2 (TLN1; TLN2) detection. Tested with WB in Human;mouse;rat;chicken. No cross reactivity with other proteins.
Cite This Product Anti-Talin Antibody (Monoclonal, 8d4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1092)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen Purified chicken gizzard talin.
Reactivity Chicken, Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Western blot, 2-4μg/ml, Human, mouse, rat, chicken

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.

Images And Assay Conditions

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Boster Kit Box

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id D3ZA84
Gene Name TLN1; TLN2
Protein Name talin 1; talin 2
Alternative Names Protein Tln2 ;Tln2 ;
Subcellular Localization TLN1: Cell projection, ruffle membrane Cytoplasm, cytoskeleton Cell surface Cell junction, focaladhesion Note: Colocalizes with LAYN at the membraneruffles. Localized preferentially in focal adhesions thanfibrillar adhesions (By similarity). |Tln1: Cell projection, ruffle membrane Cytoplasm, cytoskeleton Cell surface |TLN2: Cell junction, focal adhesion. Celljunction, synapse. Cell membrane; Peripheral membrane protein;Cytoplasmic side. Cytoplasm, cytoskeleton. Note: Focal adhesionplaques and synapses.|Tln2: Cell junction, focal adhesion Cell junction, synapse Cell membrane Cytoplasm, cytoskeleton Note: Focaladhesion plaques and synapses.
Molecular Weight 271684 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function TLN1: Probably involved in connections of major cytoskeletalstructures to the plasma membrane. High molecular weightcytoskeletal protein concentrated at regions of cell-substratumcontact and, in lymphocytes, at cell-cell contacts (Bysimilarity). |TLN2: As a major component of focal adhesion plaques thatlinks integrin to the actin cytoskeleton, may play an importantrole in cell adhesion. Recruits PIP5K1C to focal adhesion plaquesand strongly activates its kinase activity (By similarity).
Research Areas Actin Assembly, Actin Etc, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Signal Transduction

*You can search these to find other products in these research areas.
Background Talin is a high-molecular-weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts. Talin serves a function of linking vinculin to the integrins, and, thus, the cytoskeleton to extracellular matrix(ECM) receptors. It has a mass of 270 kD and shares 23% N-terminal identity with ezrin, which has similar functions. TLN gene is mapped to 9p.

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Order Product (MA1092)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1050 $240
100ug+Free Biotin Secondary BA1001 $240

USD $240

Ships next business day.

Troubleshooting

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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to talin 1 antibody, talin antibody, talin-1 antibody