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Product Info Summary
| SKU: | MA1092 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Chicken, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-Talin TLN2 Antibody (Monoclonal, 8d4)
SKU/Catalog Number
MA1092
BM1603 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Talin TLN2 Antibody (Monoclonal, 8d4) catalog # MA1092. Tested in WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Talin TLN2 Antibody (Monoclonal, 8d4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1092)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
8d4
Isotype
Mouse IgG1
Immunogen
Purified chicken gizzard talin.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1092 is reactive to TLN2 in Chicken, Human, Mouse, Rat
Observed Molecular Weight
272 kDa
Background of TLN2
Talin is a high-molecular-weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts. Talin serves a function of linking vinculin to the integrins, and, thus, the cytoskeleton to extracellular matrix (ECM) receptors. It has a mass of 270 kD and shares 23% N-terminal identity with ezrin, which has similar functions. TLN gene is mapped to 9p.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1092 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.5-1μg/ml, Human, mouse, rat, chicken
Positive Control
WB: human Daudi whole cell, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Western blot analysis of Talin using anti-Talin antibody (MA1092).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates,
Lane 2: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Talin antigen affinity purified monoclonal antibody (Catalog # MA1092) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Talin at approximately 272 kDa. The expected band size for Talin is at 272 kDa.
Specific Publications For Anti-Talin TLN2 Antibody (Monoclonal, 8d4) (MA1092)
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