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YAP1 antibody (PB9967) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat, Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 11 Publications
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Flow Cytometry analysis of U251 cells using anti-YAP1 antibody (PB9967).
Overlay histogram showing U251 cells stained with PB9967 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-YAP1 Antibody (PB9967, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U251 cells stained with PB9967 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-YAP1 Antibody (PB9967, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-YAP1 Antibody Picoband®
YAP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat, Zebrafish | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
YAP1 antibody was positively detected by WB in:
human HepG2 whole cell, human RT4 whole cell, human PC-3 whole cell, mouse RM1 whole cell, rat RH35 whole cell, rat C6 whole cell, mosue HEPA1-6 whole cell, zebrafish head tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
YAP1 antibody was positively detected by IHC in:
human invasive urothelial carcinoma of the bladder with squamous differentiation tissue, human renal clear cell carcinoma tissue, human diffuse large B-cell lymphoma of the intestine tissue, human glioblastoma tissue, human thyroid tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
YAP1 antibody was positively detected by ICC/IF in:
A549 cell, U2OS cell
YAP1 antibody was positively detected by FC in:
HepG2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Immunoprecipitation
0.5-2 μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
IP
Human
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Rat
Tested
Recommend
Zebrafish
Tested
Recommend
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-YAP1 Antibody Picoband®
View all YAP1 Antibodies
View all YAP1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human YAP1, identical to the related mouse and rat sequences.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
75 kDa
Calculated Molecular Weight
54.5 kDa
Background of YAP1
YAP1, also known as YAP or YAP65, is a potent oncogene, which is amplified in various human cancers. This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. It is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.
Cite This Product
Anti-YAP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9967)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Zebrafish
83%
Moderate Homology
Rabbit
69%
Low homology
Drosophila
50%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for YAP1 antibody
Immunohistochemistry (IHC) protocol for YAP1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for YAP1 antibody
Flow Cytometry (FC) protocol for YAP1 antibody
Publications
Application:
Species:
Reactive oxygen species drive wound epithelium and blastema formation in regenerating tail of Scincella tsinlingensis
Published: 2026-06-02
Journal: Frontiers in Zoology
Application: IF
Impact Factor: 2.80
Species: Scincella tsinlingensis
PMID: 42231459
mechanical stress-mediated ferritinophagy aggravates cartilage endplate degeneration via Piezo1-NCOA4-ZBP1 axis
Published: 2026-05-20
Journal: Autophagy
Application: WB
Impact Factor: 18.60
Species: Mouse
PMID: 42153663
KCNJ15 inhibits chemical-induced lung carcinogenesis and progression through GNB1 mediated Hippo pathway
Published: 2024-12-25
Journal: TOXICOLOGY
Application: WB
Impact Factor: 4.80
Species: Human
PMID: 39725264
Gene-engineered polypeptide hydrogels with on-demand oxygenation and ECM-cell interaction mimicry for diabetic wound healing
Published: 2024-12-02
Journal: BIOMATERIALS
Application: WB
Impact Factor: 12.80
Species: Mouse,Human,Rat
PMID: 39644880
Alpha-lipoic Acid Prevents Bone Loss in Type 2 Diabetes and Postmenopausal Osteoporosis Coexisting Conditions by Modulating the YAP/Glut4 Pathway
Published: 2024-01-23
Journal: CELL BIOCHEMISTRY AND BIOPHYSICS
Application: WB,IF
Impact Factor: 1.80
Species: Mouse
PMID: 38261247
Synthesis, biological activity and mechanism of action of novel allosecurinine derivatives as potential antitumor agents
Published: 2023-03-08
Journal: BIOORGANIC & MEDICINAL CHEMISTRY
Application: WB
Impact Factor: 3.50
Species: Human
PMID: 36906964
Periodic Heat Stress Licenses EMSC Differentiation into Osteoblasts via YAP Signaling Pathway Activation
Published: 2022-03-18
Journal: Stem Cells International
Application: WB
Impact Factor: 5.13
Species: Rat
PMID: 35355590
Effect of Gestational Diabetes on Postpartum Depression-like Behavior in Rats and Its Mechanism
Published: 2022-03-14
Journal: Nutrients
Application: WB
Impact Factor: 6.71
Species: Rat
PMID: 35334886
Enhanced Bone Regeneration Using a ZIF-8-Loaded Fibrin Composite Scaffold
Published: 2021-12-28
Journal: MACROMOLECULAR BIOSCIENCE
Application: WB,IF
Impact Factor: 4.98
Species: Rat
PMID: 34962099
YAP accelerates vascular senescence via blocking autophagic flux and activating mTOR
Published: 2020-12-12
Journal: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Application: IF,WB
Impact Factor: 4.49
Species: Rat,Human
PMID: 33314583
Identification and differentiation therapy strategy of pterygium in vitro
Published: 2018-08-15
Journal: American Journal of Translational Research
Application: IF
Impact Factor: 3.06
Species: Mouse
PMID: 30210698
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Product Promise
We promise Anti-YAP1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9967
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026