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- Table of Contents
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3 Citations
Facts about Multidrug resistance-associated protein 1.
Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficacy.
Human | |
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Gene Name: | ABCC1 |
Uniprot: | P33527 |
Entrez: | 4363 |
Belongs to: |
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ABC transporter superfamily |
ABC29; ABCC; ABCC1; ATP-binding cassette sub-family C member 1; ATP-binding cassette, sub-family C (CFTR/MRP), member 1; EC 3.6.3; EC 3.6.3.44; GS-X; Leukotriene C(4) transporter; LTC4 transporter; MRP1; MRP1DKFZp781G125; MRPDKFZp686N04233; multidrug resistance associated protein 1; multidrug resistance protein; multidrug resistance-associated protein 1; multiple drug resistance protein 1; multiple drug resistance-associated protein
Mass (kDA):
171.591 kDA
Human | |
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Location: | 16p13.11 |
Sequence: | 16; NC_000016.10 (15949616..16143062) |
Lung, testis and peripheral blood mononuclear cells.
Cell membrane; Multi-pass membrane protein.
This article reviews the IHC-optimized polyclonal antibody , as well as the Picogram sensitivity ELISA kit for the Boster Bio Anti-MRP1/ABCC1 Marker. It also examines the clinical applications of the ABCC1 marker. Picoband technology, which is an antigen derived from polymer that is proprietary will be addressed. It can save you up to 30 minutes of IHC.
Boster Bio Anti MRP1/ABC1 marker is made from a protein that functions as an indicator for Ji Jin, a cellular enzyme. This enzyme is a vital component of the immune system and is frequently expressed in normal tissue. A large number of solid tumors exhibit an elevated level of MRP1/ABCC1. This enzyme aids in the removal of therapeutic agents from tumor cells. The presence of this enzyme in solid tumors is a negative indicator for small-cell lung cancer as well as breast cancer. Its level can also indicate a response chemotherapy agents.
The Boster Bio Anti MRP1/ABCDC1 Marker consists of an antibody monoclonal to the human MRP1/ABCC1 protein but does not cross-react to MRP2 or MRP3 proteins. The monoclonal antibody can be generated by immunizing animals using an synthetic peptide that is similar to the amino acids surrounding Val273 of the human MRP1 proteins.
The multidrug resistant associated protein 1 is part of the ABC transporter superfamily. It is an essential component of the multidrug-resistant process. This protein is a multi-specific organic anion transporter and can transport glucuronides and glutathione conjugates, steroid hormones and Bile salts. It also plays a variety of functions in the immune system.
The process of analyzing tumor tissue is quick and simple with an IHC optimized antigens that are polyclonally bound to the ABCC1 marker. FFPE sections of human colon cancer exhibit the expression of p21 in abundance, which is an antigen of the ABCC1 gene. Despite the low affinity, the antibody recognizes the p21. Antigens that contain p21 bind to many IgG molecules and act as adjuvants thus enhancing the immune response.
When using an IHC-optimized polyclonally-produced antibody, the choice of the primary antibody is critical, as it will affect the final results of the experiment. First, determine the specificity of the antibody to the epitope you are interested in. Next, optimize the various steps of the IHC-processing process to minimize background signals. The working dilution for monoclonal antibodies is low due to the fact that polyclonal antibodies possess a high affinity to certain proteins. The antibody should show minimal cross-reactivity.
The concentration of the primary antibody is an important factor in achieving high-quality staining. However, it is crucial to remember that monoclonal antibodies contain a specific epitope and may show weak binding to non-antigen proteins. This background signal may hinder the detection of the target antibody, and blocking buffers are necessary to remove any background signals.
IHC is a valuable tool in disease diagnosis as well as in research and development of drugs. It is able to detect tumor cells in many tissues, and also provide predictive and prognostic information. It also can detect molecular alterations such as ATRX or IDh2 mutations. ABCC1 is a case in point. ABCC1 was previously discovered to have chromogenic IHC.
Another important factor in the selection of an IHC-optimized polyclonally-purified ABCC1 antisera is its affinity purification. The affinity purified antibody is less sensitive and reduces background staining. The antibody is created by passing through an affinity column derived from a polyclonal Antiserum. The specific antibody is attached to the immobilized antigen while the non-specific antibody is able to be eluted. As a result, the antigen-specific antibody is enhanced.
Picogram sensitivity ELISA Kit for Human Connecting Peptide (hCP) allows for quantitative determination of low levels in serum or plasma. It is designed for research purposes only. Because its half-life is 3-4 times that of insulin, this marker functions as a reliable measure of beta-cell production. Hence, it is an effective biomarker in the treatment of diabetes.
There are many polymorphisms in the ABCC1 gene. This includes the Arg723Gln and arg2168G>A variants. These polymorphisms could be linked to the growth of lung cancer within the Chinese population. People who are older are more likely to have the Arg723Gln mutation. Studies on functional aspects are needed to confirm these associations.
The MRP1/ABCC1 gene has been found to be the principal reason for multidrug resistance in both experimental and clinical contexts. Recent studies have revealed four common SNPs in mainland China. These four candidate SNPs were tested by site-directed mutagenesis to assess their impact on MRP1/ABCC1 gene expression and function. The proteins were also detected using real-time PCR Western Blot, and confomicroscopy.
In CHL patients, overexpression of ABCC1 was associated with poorer treatment outcomes and greater growth of tumors, and even mortality. This study also demonstrated an association between KIT gene mutations and imatinib responses in patients suffering from digestive tumors that originate from stroma. In addition the ABCC1 gene expression was associated with poorer patient survival with conventional frontline chemotherapy. While this isn't conclusive, it does point to the possibility of therapy targeting ABCC1.
It is essential to note that the ABCC1 marker is in contact with an MDR-associated AABtransporter. Therefore, the ABCC1 inhibitor MK571 could inhibit betulin-conferred resistance. Another study looked at the effects of overexpression of ABCB1 on cell viability after betulin treatment. Moreover, the effect of MK571 on the betulin transport was discovered to be a direct effect of the alteration in the ABCC1 subunit.
The ABCC1 marker can be a useful tool for individualized drug treatment. Many drugs are at risk of ABC transporters. This could allow for the personalization of drug treatment by substrate. The ABCC1 marker is a crucial tool in the clinical application. However, there is still much to be discovered about its structure and function and the substantial genetic variation that determines its expression. Its potential as a therapeutic target is exciting.
The ABCC1 marker was also found to be sensitive to medications in human skin carcinoma cells. The inhibitors were able to lower the expression of ABCC1 within the KB-3-1 cell line. However this resistance was not evident in Triapine treatment. This suggests that ABCC1 is extremely sensitive to COTI-2. This finding could have implications for the treatment of many diseases. The ABCC1 marker can improve the detection of many diseases, like skin cancer.
PMID: 1360704 by Cole S.P.C., et al. Overexpression of a transporter gene in a multidrug-resistant human lung cancer cell line.
PMID: 8098549 by Cole S.P.C., et al. Multidrug resistance-associated protein: sequence correction.
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