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Product Info Summary
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Anti-MRP1/ABCC1 Antibody Picoband™
Boster Bio Anti-MRP1/ABCC1 Antibody Picoband™ catalog # A00872. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRP1/ABCC1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00872)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the C-terminus of human MRP1 (1493-1528aa DYTRVIVLDKGEIQEYGAPSDLLQQRGLFYSMAKDA), different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A00872 is reactive to ABCC1 in Human
A00872 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MRP1 using anti-MRP1 antibody (A00872).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: HELA whole cell lysates,
lane 2: A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRP1 antigen affinity purified polyclonal antibody (Catalog # A00872) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRP1 at approximately 220KD. The expected band size for MRP1 is at 220KD.
Gene/Protein Information For ABCC1 (Source: Uniprot.Org, NCBI)
Multidrug resistance-associated protein 1
ABC transporter superfamily
ABC29; ABCC; ABCC1; ATP-binding cassette sub-family C member 1; ATP-binding cassette, sub-family C (CFTR/MRP), member 1; EC 3.6.3; EC 220.127.116.11; GS-X; Leukotriene C(4) transporter; LTC4 transporter; MRP1; MRP1DKFZp781G125; MRPDKFZp686N04233; multidrug resistance associated protein 1; multidrug resistance protein; multidrug resistance-associated protein 1; multiple drug resistance protein 1; multiple drug resistance-associated protein ABCC1 ABC29, ABCC, DFNA77, GS-X, MRP, MRP1 ATP binding cassette subfamily C member 1 multidrug resistance-associated protein 1|ATP-binding cassette transporter variant ABCC1delta-ex13|ATP-binding cassette transporter variant ABCC1delta-ex13&14|ATP-binding cassette transporter variant ABCC1delta-ex25|ATP-binding cassette transporter variant ABCC1delta-ex25&26|ATP-binding cassette, sub-family C (CFTR/MRP), member 1|LTC4 transporter|glutathione-S-conjugate-translocating ATPase ABCC1|leukotriene C(4) transporter*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ABCC1, check out the ABCC1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ABCC1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
A00872 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Characteristics of doxorubicin-selected multidrug-resistant human leukemia HL-60 cells with tolerance to arsenic trioxide and contribution of leukemia stem cells
Acetazolamide Suppresses Multi-Drug Resistance-Related Protein 1 and P-Glycoprotein Expression by Inhibiting Aquaporins Expression in a Mesial Temporal Epilepsy Rat Model
Enhanced autophagy reveals vulnerability of P-gp mediated epirubicin resistance in triple negative breast cancer cells
Enhanced combination therapy effect on paclitaxel-resistant carcinoma by chloroquine co-delivery via liposomes
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