Alkaline ceramidase 1 Antibodies

AND ACER1 ELISA kits, Alkaline ceramidase 1 Proteins

Many biological assays use antibodies to detect Alkaline ceramidase 1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Alkaline ceramidase 1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Alkaline ceramidase 1 antibodies...

We validate the specificity of these antibodies to Alkaline ceramidase 1 by testing them on tissues known to express ACER1 positively and negatively. Browse below to find the ACER1 antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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ACER1 Infographic by Boster Bio

All ACER1 Products

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Specificity

Applications

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Anti-ASAH3 Antibody

7 Q&As

Western Blot (WB) analysis of HepG2 using ASAH3 antibody

Human, Mouse

ELISA, WB

$399
Cat # A12775
100μl

Anti-Alkaline ceramidase 1 ACER1 Antibody

Western blot (WB) analysis of ACER1 polyclonal antibody at 1:500 dilution
Lane1:HepG2 whole cell lysate
Lane2:Raw264

Immunohistochemistry (IHC) analyzes of ACER1 pAb bin paraffin-embedded human liver carcinoma tissue at 1:50,showing cytoplasmic staining

Human, Mouse, Rat

IHC, WB

$405
Cat # A12775-1
100ul

ASAH3 (ACER1) (NM_133492) Human Over-expression Lysate

Human

$628
Cat # LS125981
20 µg, 100 µg

ACER1 Overview

Facts about Alkaline ceramidase 1.

ACER1 3D structure. Source: alphafold

Alkaline ceramidase 1 (ACER1)

Hydrolyzes the sphingolipid ceramide to sphingosine and free fatty acid for an optimal pH of 8.0.

Has an extremely restricted substrate specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine but not D-ribo- phytosphingosine or D-erythro-dihydrosphingosine as a backbone. May have a role in regulating the levels of bioactive lipids ceramide and sphingosine 1-phosphate, in addition to complex sphingolipids (By similarity).

Gene Information

Human
Gene Name:	ACER1
Uniprot:	Q8TDN7
Entrez:	125981

Family

Belongs to:

alkaline ceramidase family

Alternative Names

Acylsphingosine deacylase 3; Alkaline CDase 1; alkaline ceramidase 1; AlkCDase 1; ALKCDase1; ASAH3; EC 3.5.1.23; MGC138327; MGC138329; N-acylsphingosine amidohydrolase (alkaline ceramidase) 3; N-acylsphingosine amidohydrolase 3

Molecular Weight

Mass (kDA):
31.095 kDA

Genomic Context


Human

Location:	19p13.3
Sequence:	19; NC_000019.10 (6306142..6360368, complement)

Tissue Specificity

Mainly expressed in epidermis.

Subcellular Localizations

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Diseases

Farber Lipogranulomatosis
Neoplasms
Growth Arrest
Growth Retardation
Sphingolipidoses
Leukemia
Acute Promyelocytic Leukemia

Tissue Adhesions
Tuberculosis

Pathways

Cell Proliferation
Transport
Intracellular Transport
Cell Death
Cell Cycle
Cell Differentiation
Cell Growth
Keratinocyte Differentiation

Rna Interference
Cell Cycle Arrest

PTMs

Phosphorylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ACER1

References

PMID: 12783875 by Mao C., et al. Cloning and characterization of a mouse endoplasmic reticulum alkaline ceramidase: an enzyme that preferentially regulates metabolism of very long chain ceramides.

PMID: 16477081 by Houben E., et al. Differentiation-associated expression of ceramidase isoforms in cultured keratinocytes and epidermis.