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Product Info Summary
| SKU: | A12775 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ASAH3 Antibody
SKU/Catalog Number
A12775
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ASAH3 Antibody catalog # A12775. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ASAH3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12775)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from ASAH3 . at AA range: 100-180
Reactive Species
A12775 is reactive to ACER1 in Human, Mouse
Calculated molecular weight
31.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12775 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HepG2 using ASAH3 antibody.
Specific Publications For Anti-ASAH3 Antibody (A12775)
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Customer Q&As
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7 Customer Q&As for Anti-ASAH3 Antibody
Question
Would anti-ASAH3 antibody A12775 work on primate WB with skin of leg?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
Our lab technicians have not validated anti-ASAH3 antibody A12775 on primate. You can run a BLAST between primate and the immunogen sequence of anti-ASAH3 antibody A12775 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate skin of leg in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-01
Question
I am interested in to test anti-ASAH3 antibody A12775 on human skin of leg for research purposes, then I may be interested in using anti-ASAH3 antibody A12775 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
The products we sell, including anti-ASAH3 antibody A12775, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-10
Question
I was wanting to use your anti-ASAH3 antibody for WB for human skin of leg on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human skin of leg identification?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
It shows on the product datasheet, A12775 anti-ASAH3 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human skin of leg in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-03
Question
Is a blocking peptide available for product anti-ASAH3 antibody (A12775)?
Verified Customer
Verified customer
Asked: 2019-02-12
Answer
We do provide the blocking peptide for product anti-ASAH3 antibody (A12775). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-02-12
Question
We are currently using anti-ASAH3 antibody A12775 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
The anti-ASAH3 antibody (A12775) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-26
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin of leg using anti-ASAH3 antibody A12775. Let me know if you need anything else.
W. Krishna
Verified customer
Asked: 2014-06-13
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-06-13
Question
Would A12775 anti-ASAH3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Zhao
Verified customer
Asked: 2013-01-30
Answer
You can see on the product datasheet, A12775 anti-ASAH3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-01-30
