Anti-ASAH3 Antibody

ASAH3 antibody

Boster Bio Anti-ASAH3 Antibody catalog # A12775. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A12775
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-ASAH3 Antibody

View all ASAH3 Antibodies

SKU/Catalog Number

A12775

Size

100μl

Form

Liquid

Description

Boster Bio Anti-ASAH3 Antibody catalog # A12775. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ASAH3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12775)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human ASAH3. at AA rangle: 100-180

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12775 is reactive to ACER1 in Human

Applications

A12775 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

31.095kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For ACER1 (Source: Uniprot.org, NCBI)

Gene Name

ACER1

Full Name

Alkaline ceramidase 1

Weight

31.095kDa

Superfamily

alkaline ceramidase family

Alternative Names

Acylsphingosine deacylase 3; Alkaline CDase 1; alkaline ceramidase 1; AlkCDase 1; ALKCDase1; ASAH3; EC 3.5.1.23; MGC138327; MGC138329; N-acylsphingosine amidohydrolase (alkaline ceramidase) 3; N-acylsphingosine amidohydrolase 3 ACER1 ALKCDase1, ASAH3 alkaline ceramidase 1 alkaline ceramidase 1|CTB-180A7.3|N-acylsphingosine amidohydrolase (alkaline ceramidase) 3|N-acylsphingosine amidohydrolase 3|acylsphingosine deacylase 3|alkCDase 1|alkaline CDase 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACER1, check out the ACER1 Infographic

ACER1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACER1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-ASAH3 Antibody

Question

Would anti-ASAH3 antibody A12775 work on primate WB with skin of leg?

Verified Customer

Verified customer

Asked: 2019-10-01

Answer

Our lab technicians have not validated anti-ASAH3 antibody A12775 on primate. You can run a BLAST between primate and the immunogen sequence of anti-ASAH3 antibody A12775 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate skin of leg in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-01

Question

I am interested in to test anti-ASAH3 antibody A12775 on human skin of leg for research purposes, then I may be interested in using anti-ASAH3 antibody A12775 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-09-10

Answer

The products we sell, including anti-ASAH3 antibody A12775, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-10

Question

I was wanting to use your anti-ASAH3 antibody for WB for human skin of leg on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human skin of leg identification?

Verified Customer

Verified customer

Asked: 2019-09-03

Answer

It shows on the product datasheet, A12775 anti-ASAH3 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human skin of leg in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-03

Question

Is a blocking peptide available for product anti-ASAH3 antibody (A12775)?

Verified Customer

Verified customer

Asked: 2019-02-12

Answer

We do provide the blocking peptide for product anti-ASAH3 antibody (A12775). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-02-12

Question

We are currently using anti-ASAH3 antibody A12775 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2017-05-26

Answer

The anti-ASAH3 antibody (A12775) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-05-26

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin of leg using anti-ASAH3 antibody A12775. Let me know if you need anything else.

W. Krishna

Verified customer

Asked: 2014-06-13

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-06-13

Question

Would A12775 anti-ASAH3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

D. Zhao

Verified customer

Asked: 2013-01-30

Answer

You can see on the product datasheet, A12775 anti-ASAH3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-01-30

Size

Total: $399

SKU:A12775

Backordered.

Lead time for this item is typically 2-4 weeks

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A12775
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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