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1 Citations 1 Q&As
1 Citations 6 Q&As
Facts about ATP-citrate synthase.
In nervous tissue it might be involved in the biosynthesis of acetylcholine. .
Human | |
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Gene Name: | ACLY |
Uniprot: | P53396 |
Entrez: | 47 |
Belongs to: |
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No superfamily |
ACLEC 2.3.3.8; ATP citrate lyase; ATP citrate synthase; ATP-citrate (pro-S-)-lyase; ATP-citrate synthase; ATPCL; Citrate cleavage enzyme; CLATP
Mass (kDA):
120.839 kDA
Human | |
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Location: | 17q21.2 |
Sequence: | 17; NC_000017.11 (41866916..41930542, complement) |
Cytoplasm, cytosol.
If you've been pondering what Boster Bio's ACLY marker does, then this article is suitable for you. In this article, we'll discuss about the ACLY marker's uses, how to use it to determine the protein you want to target, and how to find relevant infographics about genes. Read on to learn more about the ACLY marker! These are the Best Uses of The ACLY Marker
Boster Bio's ACLY marker for anti-ATP citrate is a high-quality monoclonal anti-rabbit antigen for ATP citrate lysis. Picoband(tm) which contains the ACLY marker reacts with both mice & rat. To test this antibody, mix equal portions of mouse and rat serum. The antibody will then react with ATP citrate cleavage protein.
ATP citrate lysis is an important biological process that is a key step in the biosynthesis of acetyl CoA in human cells. This enzyme is located in the cytosol. It is the principal enzyme responsible for the creation of coA acetyl. ATP citrate lyase is composed of two forms, one which is found in cancer cells and the other in normal lung parenchyma.
ACLY (adenomatous polyposis coli) is a mRNA found in cells. Its expression in tissues is up-regulated by RT-PCR and immunoblotting. The protein is more abundant in RCC tissues than in normal tissues that are adjacent and the ACLY levels in serum are not significantly different between people with RCC and those who are not. The ACLY small interferingRNA-mediated down-regulation does not hinder cell proliferation, but it does promote anapoptosis. These results were in line with previous studies.
In most types of cancer there is an increased rate of lipogenesis and glycolysis. These higher rates can have an impact on the expression of some proteins and molecular expression. ATP citrate lyase is one of these proteins. It acts as a physiological shunt between glucose metabolic and fatty acid synthesis. ACLY is necessary for lipogenesis and is a major element of the cell's energy metabolism. When overexpressed, ACLY suppresses the synthesis of lipids in oxygenated cells and restores glycolysis inhibition in hypoxic cells.
Total RNA was extracted from tissue samples and reverse transcription was done using SYBR Premix Ex Taq kits. Primers were human ACLY and B–actin. Additionally, we employed the Livak-Schmittgen technique for DDCq. All values are relative with an unreferenced DCq value. This approach was used in our study to determine the expression of ACLY in humans during apoptosis.
The ACLY marker is able to detect high levels of Acetyl-CoA. This is a crucial compound that regulates global histone acetylation. It functions as a physiological shunt between glucose metabolism and fatty acid synthesis. It also promotes the process of apoptosis. Utilizing ACLY in research has numerous potential applications. For instance, this biomarker has the potential to help in early detection of prostate cancer, which is closely linked to it.
For researchers who are looking to understand the role of genes in human health the Gene infographics of Boster Bio are vital. This tool gives basic information about every gene found in human and mouse species. With a search bar, researchers can locate the gene they want to study. Gene infographics can also be printed out for reference. For more information about the features of the Gene infographics for Boster Bio, read on.
PMID: 1371749 by Elshourbagy N.A., et al. Cloning and expression of a human ATP-citrate lyase cDNA.
PMID: 9116495 by Lord K.A., et al. Variant cDNA sequences of human ATP:citrate lyase: cloning, expression, and purification from baculovirus-infected insect cells.
*More publications can be found for each product on its corresponding product page