Anti-ATP citrate lyase ACLY Antibody Picoband™ (monoclonal, 5I2)

Boster Bio Anti-ATP citrate lyase ACLY Antibody Picoband™ (monoclonal, 5I2) catalog # M02372-1. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: M02372-1
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-ATP citrate lyase ACLY Antibody Picoband™ (monoclonal, 5I2)

See all ATP Citrate Lyase products

SKU/Catalog Number

M02372-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ATP citrate lyase ACLY Antibody Picoband™ (monoclonal, 5I2) catalog # M02372-1. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ATP citrate lyase ACLY Antibody Picoband™ (monoclonal, 5I2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02372-1)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

5I2

Isotype

IgG2b

Immunogen

E. coli-derived human ATP citrate lyase recombinant protein (Position: M1-I180). Human ATP citrate lyase shares 95% amino acid (aa) sequence identity with both mouse and rat ATP citrate lyase.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M02372-1 is reactive to ACLY in Human, Mouse, Rat

Applications

M02372-1 is guaranteed for Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For ACLY (Source: Uniprot.Org, NCBI)

Uniprot ID

P53396

Gene ID

47

Gene Name

ACLY

Full Name

ATP-citrate synthase

Weight

120.839kDa

Alternative Names

ACLEC 2.3.3.8; ATP citrate lyase; ATP citrate synthase; ATP-citrate (pro-S-)-lyase; ATP-citrate synthase; ATPCL; Citrate cleavage enzyme; CLATP ACLY ACL, ATPCL, CLATP ATP citrate lyase ATP-citrate synthase|ATP-citrate (pro-S-)-lyase|citrate cleavage enzyme

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ACLY, check out the ACLY Infographic

ACLY infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ACLY: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M02372-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Huang Y,Zhao C,Kong Y,Tan P,Liu S,Liu Y,Zeng F,Yuan Y,Zhao B,Wang J.Elucidation of the mechanism of NEFA-induced PERK-eIF2α signaling pathway regulation of lipid metabolism in bovine hepatocytes.J Steroid Biochem Mol Biol.2021 Apr 2:105893.doi:10.101 6/j.jsbmb.2021.105893.Epub ahead of print.PMID:33819629.
Species: Holstein calves
M02372-1 usage in article: APP:WB, SAMPLE:HEPATOCYTES, DILUTION:1:1000

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6 Customer Q&As for Anti-ATP citrate lyase ACLY Antibody Picoband™ (monoclonal, 5I2)

Question

Is there a BSA free version of anti-ATP citrate lyase antibody (monoclonal, 5I2) M02372-1 available?

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ATP citrate lyase antibody (monoclonal, 5I2) M02372-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-05-01

Question

See below the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-ATP citrate lyase antibody (monoclonal, 5I2) M02372-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-14

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-14

Question

I was wanting to use your anti-ATP citrate lyase antibody (monoclonal, 5I2) for IHC-P for mouse cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma erythroleukemia identification?

Verified Customer

Verified customer

Asked: 2019-05-10

Answer

You can see on the product datasheet, M02372-1 anti-ATP citrate lyase antibody (monoclonal, 5I2) has been validated for Flow Cytometry, IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-10

Question

I see that the anti-ATP citrate lyase antibody (monoclonal, 5I2) M02372-1 works with IHC-P, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-04-24

Answer

You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-04-24

Question

Would anti-ATP citrate lyase antibody (monoclonal, 5I2) M02372-1 work for IHC-P with cervix carcinoma erythroleukemia?

L. Williams

Verified customer

Asked: 2017-12-08

Answer

According to the expression profile of cervix carcinoma erythroleukemia, ACLY is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-ATP citrate lyase antibody (monoclonal, 5I2) M02372-1 will work for IHC-P with cervix carcinoma erythroleukemia.

Boster Scientific Support

Answered: 2017-12-08

Question

I am interested in to test anti-ATP citrate lyase antibody (monoclonal, 5I2) M02372-1 on mouse cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-ATP citrate lyase antibody (monoclonal, 5I2) M02372-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

E. Banerjee

Verified customer

Asked: 2016-10-17

Answer

The products we sell, including anti-ATP citrate lyase antibody (monoclonal, 5I2) M02372-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2016-10-17

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M02372-1
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