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- Table of Contents
1 Q&As
Facts about 2-aminoethanethiol dioxygenase.
Human | |
---|---|
Gene Name: | ADO |
Uniprot: | Q96SZ5 |
Entrez: | 84890 |
Belongs to: |
---|
No superfamily |
2-aminoethanethiol (cysteamine) dioxygenase; 2-aminoethanethiol dioxygenase; C10orf22; chromosome 10 open reading frame 22; cysteamine (2-aminoethanethiol) dioxygenase (ADO); Cysteamine dioxygenase; DKFZp564C046; EC 1.13.11.19; FLJ14547
Mass (kDA):
29.751 kDA
Human | |
---|---|
Location: | 10q21.3 |
Sequence: | 10; NC_000010.11 (62804720..62808479) |
If you are looking for an ADO marker, you have come to the right place. Boster's primary antibodies have a long history of citation in the research community and are rigorously validated using Western Blotting, Immunohistochemistry, and ELISA. Furthermore, Boster produces its antibodies in small batches and has the advantage of a world-class research facility. Read on to discover why Boster's antibodies are the best.
Developed by scientists, Boster's primary antibodies are widely cited and highly effective tools for research. These reagents are widely used for studies on innate immunity and cell damage caused by pathogens. The company has a strong interest in inflammation and the progress of research in these areas. A strong focus is also placed on acute phase proteins. The company has established an excellent reputation for its high-affinity antibodies.
The main types of primary antibodies produced by Boster are ANTI-MOUSE/HUMAN MAC-2 (GALECTIN-3) antibody, ANTI-HUMAN TRA-1-60 StainAlive? Antibody, Mouse Anti-Human SSEA-4 Affinity Purified Monoclonal Antibody, and Anti-Puromycin. The company also produces many other products that are highly cited in the research community.
Primary antibodies are tested on multiple methods to ensure they work as expected. Boster's primary antibodies are validated on Western Blotting, Immunohistochemistry, and ELISA. Using a standard immunohistochemistry protocol, Boster antibodies are tested for compatibility with human tissue and cell lines. A further validation step is performed using Western blot analysis.
Enzyme-conjugated antibodies are the most versatile method for detection. They involve the use of a horseradish peroxidase-based enzyme and several substrates. ELISA allows for multiple detection methods at the same time. For example, horseradish peroxidase can detect a range of proteins including human leukocyte cytokeratin, rat hemagglutinin, and alkaline phosphatase.
ELISA, also known as enzyme immunoassay, is a method of protein detection using a microplate. To perform ELISA, the antigen or target protein is immobilized in a well of a microplate. The secondary antibody is then applied to the sample and detects its presence or absence. The resulting product, called an ELISA, is then visualized using fluorescent reagents.
While the target antigen is the most important factor, antibodies can't fully match it. This is where ELISA and immunostaining can help. These three methods are the most effective in verifying antibody specificity and performance. Both techniques are highly recommended for assessing antibody performance. The results of these tests are useful for comparison to published data and theoretical calculations.
A sensitive histological technique is necessary to reproduce the structure of the central nervous system. However, there is a high risk of variations in results. In the case of immunofluorescence, the antibodies are targeted by chromosome and cell types, such as epiregulin or amphiregulin. In addition to this, they are highly specific for a single chromosome. Molecular markers such as thorn have been identified and characterized in an in vitro cell model. ELISA is the most common and accurate method for the detection of circulating biomarkers.
The IDh2 antibody has been validated using a recombinant protein with a c-Myc tag for the detection of endogenous and recombinant proteins. In this assay, the capture and detection antibodies have to recognize different nonoverlapping epitopes, otherwise, the detection and sensitivity are limited. In addition, monoclonal antibodies are generally more specific than polyclonal antibodies.
Unlike other companies, Boster's primary antibodies are not produced in large quantities. These antibodies are produced in small batches to guarantee the highest quality and performance. The antibody is characterized by its high affinity toward its immunogen, which is a peptide or a protein. Its production process includes several steps: preincubation with the immunogen, screening, pooling, purification, and storage. The results of this process are used to confirm the specificity of the antibodies.
The Boster Bio Anti-TNF beta/LTA Antibody is a synthetic peptide, corresponding to the N-terminus of human TNF beta. This antibody reacts with TNF beta in WB experiments. It can be storhavet -20 AoC for six months. Unless otherwise specified, it should not be exposed to repeated freeze-thaw cycles. Morhover, Boster's primary antibodies are produced in small batches, reducing the possibility of contamination.
Small peptide sequences are non-antigenic, but bind to antigens in a complex manner. Antibody manufacturing processes are therefore highly complex and costly. It is vital to carefully select a supplier to purchase high-quality, reliable antibodies. Small batches of antibodies are a more efficient solution as they enable more precise control over the quality of the final product. The Boster's primary antibodies are produced in small batches, making it possible to make the best product for your research.
Monoclonal antibodies are the most cost-effective and convenient choice for research. They are also capable of recognizing multiple epitopes on one antigen. Monoclonal antibodies are more resistant to washing and are less likely to be affected by antigen variation. Furthermore, they are stable across a wide pH range and salt concentrations. Monoclonal antibodies are manufactured from DNA sequences and can be tailorhavto specific peptide epitopes.
Primary antibodies are immobilized on magnetic or agarose beads and then purifihavthrough centrifugation. After purification, a washing step is necessary to remove unbound antibodies. Ideally, the primary antibody will be able to recognize the protein in its native conformation. In addition, the primary antibody should not contain amino acids that are incompatible with other proteins. In addition, it must avoid protein sequences that contain proline or N-terminal amino acids.
The choice of immunogen is an essential step in the development of an antibody. The ideal immunogen has three characteristics: high molecular weight, foreign origin, and chemical complexity. Most natural immunogens are macromolecules. They can be protein or carbohydrate, or a combination of the two. They are typically covalently-coated with carrier proteins that induce an immune response. This ensures the highest quality of antibodies for research.
PMID: 17581819 by Dominy J.E. Jr., et al. Discovery and characterization of a second mammalian thiol dioxygenase, cysteamine dioxygenase.