Anti-ADO Antibody Picoband™

ADO antibody

Boster Bio Anti-ADO Antibody Picoband™ catalog # PB10032. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Independently reviewed in 1 review(s).

Product Info Summary

SKU: PB10032
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-ADO Antibody Picoband™

View all ADO Antibodies

SKU/Catalog Number

PB10032

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ADO Antibody Picoband™ catalog # PB10032. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ADO Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10032)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human ADO recombinant protein (Position: E49-E261). Human ADO shares 90.1% amino acid (aa) sequence identity with mouse ADO.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB10032 is reactive to ADO in Human, Mouse, Rat

Applications

PB10032 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

28 kDa, 30 kDa

Calculated molecular weight

29.751kDa

Background of ADO

Human thiol dioxygenases include cysteine dioxygenase (CDO) and cysteamine (2-aminoethanethiol) dioxygenase (ADO). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. It is demonstrated that mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. It is also noted that the demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For ADO (Source: Uniprot.org, NCBI)

Gene Name

ADO

Full Name

2-aminoethanethiol dioxygenase

Weight

29.751kDa

Alternative Names

2-aminoethanethiol (cysteamine) dioxygenase; 2-aminoethanethiol dioxygenase; C10orf22; chromosome 10 open reading frame 22; cysteamine (2-aminoethanethiol) dioxygenase (ADO); Cysteamine dioxygenase; DKFZp564C046; EC 1.13.11.19; FLJ14547 ADO C10orf22 2-aminoethanethiol dioxygenase 2-aminoethanethiol dioxygenase|2-aminoethanethiol (cysteamine) dioxygenase|cysteamine (2-aminoethanethiol) dioxygenase (ADO)|cysteamine dioxygenase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ADO, check out the ADO Infographic

ADO infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ADO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for PB10032

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-ADO Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

1 Reviews For Anti-ADO Antibody Picoband™

0

ADO Antibody Works in Western Blot--Hua Jiang, Pediatrics, University of Colorado Denver, Research Associate

Excellent

Source: Biocompare.com

Applications Western Blot
Sample Human liver, rat liver, mouse liver, mouse kidney, and mouse brain
Detection Typhoon 940

"We are studying the metabolism of amino acid and we are interested in knowing if the pathway involved this protein has changed under the background of a knockout mouse. The western blot image for this antibody looks clean and the signal looks strong. The band shown on western blot is at the right size and the intensity is strong enough. Equally important, the price is generally lower than other companies. This antibody works ok in western blot experiments with human, rat, and mouse tissues."

Verified customer

Submitted

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

1 Customer Q&As for Anti-ADO Antibody Picoband™

Question

We are currently using anti-ADO antibody PB10032 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

S. Thomas

Verified customer

Asked: 2013-02-25

Answer

The anti-ADO antibody (PB10032) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-02-25

Size

Conjugation

Total: $370

SKU:PB10032

In stock, 1 left.

Order within 31 hours and 4 minutes to receive by Thu Mar 21

Get A Quote
Eddy test
In stock
Order Product
PB10032
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.