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Facts about E3 ubiquitin-protein ligase AMFR.
The VCP/p97- AMFR/gp78 complex is included in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complicated at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is subsequently released from the ER from the complex into the cytosol for subsequent destruction.
Human | |
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Gene Name: | AMFR |
Uniprot: | Q9UKV5 |
Entrez: | 267 |
Belongs to: |
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No superfamily |
AMF receptor; AMF receptor, isoform 1; AMF receptor, isoform 2; AMFR; autocrine motility factor receptor; Autocrine motility factor receptor, isoform 2; gp78; RING finger protein 45; RNF45; RNF45EC 6.3.2.-
Mass (kDA):
72.996 kDA
Human | |
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Location: | 16q13 |
Sequence: | 16; NC_000016.10 (56361452..56425545, complement) |
Endoplasmic reticulum membrane; Multi-pass membrane protein. Palmitoylation promotes localization to the peripheral endoplasmic reticulum.
If you're considering buying Boster Anti-AMFR Markers, you've probably been informed about Steven Boster's past as well as gene infographics and high-affinity primary antibodies. What exactly is this marker and how does it benefit you? Find out more. Here are a few of the best uses for this unique protein. But don't stop there. Read on for information on Boster's past and what's special about its anti-AMFR Marker.
Boster's Anti AMFR Markers can detect mouse, human and rat AMFR. Boster validates its antibodies by using different platforms and known positive and negative samples to confirm specificity and affinity. Boster also provides product credit to the first reviewers. This incentive encourages scientists to share their information and suggestions to improve the products of Boster. Here's a summary of Boster's Anti-AMFR Marker's capabilities.
Here's a little bit of information about Steven Boster's life. In addition Boster was a huge lover of the southern gospel and sang so low in public that the woman who was sitting next to him could not hear him. He also loved sports and automobile racing and was never late for the Friday night games at the local track and attended additional events on weekends, as well as dirt track races.
When performing immunohistochemistry, monoclonal antibodies are used to detect a protein of interest. They are less expensive and can be tested against the same antigen many times to determine a distinct epitope. They are also more stable across a wide range pH and salt concentrations. Monoclonal antibody suffers from the disadvantage of not staining samples of tissue that do not express the protein of interest.
This doesn't mean that the primary antibody must be replaced with using a PBS solution. Preimmune serum is the best choice to replace the primary antibody. An IgG from the same class could be used in place of the primary antibody if it is unavailable. These antisera won't stain the sample so it is important that you replace the primary antibody before proceeding to the next step.
It is vital that the primary antibodies bind target antigens with high affinity. This reduces the risk of nonspecific interactions. Therefore, antibodies must be used in high dilutions to reduce nonspecific interactions. Short incubation times may result in a diminished signal. It is vital that researchers have a deep understanding of the antigen that is used to immunize animals.
The ELISA method employs high-affinity antibodies to identify specific analytes from crude preparation. This method is superior to sandwich ELISA since it makes use of the matched pairs of primary antibodies. This method is quicker and more sensitive than other tests. However, it does have one major drawback. Hooking that is not specific binds the primary antibody and secondary antibodies, and reduces the signal-to-noise ratio.
In addition to being versatile, monoclonal antibodies can be extremely specific. They can recognize a single epitope in an antigen with high affinity and have low cross-reactivity. However, because of their high specificity, monoclonal antibodies require high concentrations to avoid contamination and are not resistant to washing processes. Additionally, monoclonal antibodies are also costly and have a limited shelf-life, so their usage is restricted.
PMID: 10456327 by Shimizu K., et al. The autocrine motility factor receptor gene encodes a novel type of seven transmembrane protein.
PMID: 7626106 by Huang B., et al. Identification of an upstream region that controls the transcription of the human autocrine motility factor receptor.