Anti-AMFR Antibody Picoband™

AMFR/gp78 antibody

Boster Bio Anti-AMFR Antibody Picoband™ catalog # PB10039. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB10039
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-AMFR Antibody Picoband™

View all AMFR/gp78 Antibodies

SKU/Catalog Number

PB10039

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-AMFR Antibody Picoband™ catalog # PB10039. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AMFR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10039)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human AMFR recombinant protein (Position: E553-S643). Human AMFR shares 89% amino acid (aa) sequence identity with mouse AMFR.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB10039 is reactive to AMFR in Human, Mouse, Rat

Applications

PB10039 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

73 kDa

Calculated molecular weight

72.996kDa

Background of AMFR/gp78

Autocrine motility factor receptor, isoform 2 is a protein that in humans is encoded by the AMFR gene. Autocrine motility factor is a tumor motility-stimulating protein secreted by tumor cells. The protein encoded by this gene is a glycosylated transmembrane protein and a receptor for autocrine motility factor. The receptor, which shows some sequence similarity to tumor protein p53, is localized to the leading and trailing edges of carcinoma cells. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For AMFR (Source: Uniprot.org, NCBI)

Gene Name

AMFR

Full Name

E3 ubiquitin-protein ligase AMFR

Weight

72.996kDa

Alternative Names

AMF receptor; AMF receptor, isoform 1; AMF receptor, isoform 2; AMFR; autocrine motility factor receptor; Autocrine motility factor receptor, isoform 2; gp78; RING finger protein 45; RNF45; RNF45EC 6.3.2.- AMFR GP78, RNF45 autocrine motility factor receptor E3 ubiquitin-protein ligase AMFR|RING finger protein 45|RING-type E3 ubiquitin transferase AMFR

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AMFR, check out the AMFR Infographic

AMFR infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AMFR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-AMFR Antibody Picoband™

Question

I am looking for to test anti-AMFR antibody PB10039 on rat liver for research purposes, then I may be interested in using anti-AMFR antibody PB10039 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-08-28

Answer

The products we sell, including anti-AMFR antibody PB10039, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-28

Question

We are currently using anti-AMFR antibody PB10039 for mouse tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-27

Answer

The anti-AMFR antibody (PB10039) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-27

Question

Does PB10039 anti-AMFR antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-02-01

Answer

As indicated on the product datasheet, PB10039 anti-AMFR antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-02-01

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-AMFR antibody PB10039. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-02-28

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-02-28

Question

See attached the WB image, lot number and protocol we used for liver using anti-AMFR antibody PB10039. Please let me know if you require anything else.

T. Johnson

Verified customer

Asked: 2014-01-08

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-01-08

Size

Conjugation

Total: $370

SKU:PB10039

In stock, 1 left.

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PB10039
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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