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- Table of Contents
3 Q&As
Facts about Amyloid beta A4 precursor protein-binding family B member 1-interacting protein.
Mediates Rap1- induced adhesion. .
Human | |
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Gene Name: | APBB1IP |
Uniprot: | Q7Z5R6 |
Entrez: | 54518 |
Belongs to: |
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MRL family |
amyloid beta (A4) precursor protein-binding, family B, member 1 interactingprotein; amyloid beta A4 precursor protein-binding family B member 1-interacting protein; APBB1-interacting protein 1; APBB1IP; INAG1; PREL1; PREL-1; proline rich EVH1 ligand 1; Proline-rich EVH1 ligand 1; Proline-rich protein 73; Rap1-GTP-interacting adapter molecule; Rap1-interacting adaptor molecule; RARP1; Retinoic acid-responsive proline-rich protein 1; RIAM; RIAMRARP-1
Mass (kDA):
73.183 kDA
Human | |
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Location: | 10p12.1 |
Sequence: | 10; NC_000010.11 (26438324..26567879) |
Widely expressed with high expression in thymus, spleen, lymph node, bone marrow and peripheral leukocytes.
Cell membrane; Peripheral membrane protein. Cell projection, lamellipodium. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Colocalizes with ENA/VASP proteins at lamellipodia tips and focal adhesions, and F-actin at the leading edge. At the membrane surface, associates, via the PH domain, preferentially with the inositol phosphates, PtdIns(5)P and PtdIns(3)P. This binding appears to be necessary for the efficient interaction of the RA domain to Ras-GTPases (By similarity).
This informative article discusses the following topics: Enhanced Chemiluminescence Identification (ECL), and Protein transfer efficiency using membrane staining. You can find more information about Boster in the Boster bio section: Best Uses For The APBB1IPMarker. Here are some examples of the ways these tools can help.
Enhanced Chemiluminescence activation detection is a technique that detects proteins bound to HP. The reaction between the enzyme, the chemiluminescent medium produces excited intermediates. These intermediates emit light at 450nm. The enzyme-substrate interaction is what causes the light emission. The signal output stops when the substrate is removed from the enzyme.
Previously the APBB1IP marker had been detected using a method called Enhanced Chemiluminescence Activation (ECA) in a cell cultivation dish. Several proteins were detected including TNFRSF19 and BMP4, ODAM and BAMBI, as well as ASPSCR1.
The HRP substrate, a highly sensitive and efficient enzyme, is used in the ECL process. HRP substrates from Thermo Scientific Pierce offer different levels of sensitivity. Below are some characteristics of common HRP-chemiluminescent substrates. The ECL method proves particularly useful in Western Blot applications involving many proteins.
ECL reagents are available for the detection of protein levels in cell culture. Amersham ECL Select has a high sensitivity and is perfect for detecting low to moderate protein concentrations. It is easy to use, and has a very long shelf life. This technique is highly recommended by experts and is widely used for Western blot experiments.
Rapid MBD-based colorimetric and electrochemical approaches to DNA methylation detection were successfully demonstrated in clinical samples. They feature low sample requirements and speed. These assays have potential clinical applications. We will now discuss how these assays can be used for diagnostic purposes. This article gives an overview of both the benefits and disadvantages of each method. A brief description follows.
This method is cost effective, easy to use, and offers high sensitivity. However, prolonged storage can create background signals that obscure the protein of particular interest. Low-abundance proteins may not be suitable for colorimetric detection. However, colorimetric detection is an affordable and simple method of detecting proteins. This makes it a popular choice in research studies. There are many substrates that can detect colorimetric detection, each with their own levels of sensitivity.
The 1-Step(tm), TMB substrat solution was used. The dye-based sample was then analyzed at the 650 nm wavelength using an EnSpirer(r) plate scanner. The absorbance at 20 min was used for data analysis. The APBB1IP marker serves as an indicator for APBB1 immuneassays.
Membrane stains are a great way of detecting protein bands in cell cultures. Ponceau S solution, a sensitive stain, can be used to visualize the protein bands on both PVDF and nitrocellulose membranes. Its negative charge is capable of binding to amino-acid compounds on the membrane. The dye is highly specific with a high sensitivity and low background. This staining technique is fast, simple and reversible, but it is not suitable to detect protein on nitrocellulose or nylon membranes.
The APBB1IP membrane staining procedure involves fixing cells and using Coomassie Brilliant Blue for visualization of protein bands. It is simple, inexpensive, and can be used to determine the functional activity of cells. The result is a precise representation of the protein levels in the cell. The Boster Bio APBB1IP marker for membrane staining is compatible and compatible with most protein extraction methods.
Fluorescence-based membrane staining can improve the efficiency of protein transfer in cell culture experiments. Scientists can identify the expression of specific proteins using fluorescent dyes. Fluorescent dyes can be used in flow cytometry experiments. They are specific to each cell type and emit light after they have been excited with a compatible wavelength. A variety of fluorescent dyes is now available.
In Western blotting, the primary antibody and secondary antibody must be diluted with the appropriate buffers. The primary antibody should remain at room temperature for several hours, or overnight. The time needed may need to be optimized. After incubation, the membrane should be rinsed three times with TBS Wash Buffer. Clear preservative film should be placed over the membrane to block it. To remove air bubbles, you can use transparent glass paper.
His birth is the beginning of Steve Boster's story. Steve, the father of Donald Sr. and Nina Mae Hall was born in Joliet (Illinois) on June 6, 1922. He was a U.S. Army Veteran and worked in retail sales for many decades. His legacy will continue with his two daughters, Natosha and Crystal Peck, and six grandchildren. His parents were married for over 50 years and he is survived in Herrin, IL by his son Jonathan, and grandson Cory.
Steve Boster, in his later years, was a successful entrepreneur after he developed his first product. He was soon developing products for IHC, ELISA, and hundreds of primary antibodies. His company became China's largest catalog antibody business. He was also known for his patented PicoKine(tm) ELISA platform. These developments made Boster one the most prominent companies in the field.
PMID: 14530287 by Inagaki T., et al. The retinoic acid-responsive proline-rich protein is identified in promyeloleukemic HL-60 cells.
PMID: 15469846 by Lafuente E.M., et al. RIAM, an Ena/VASP and profilin ligand, interacts with Rap1-GTP and mediates Rap1-induced adhesion.