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- Table of Contents
Cysteine protease ATG4A Antibodies
AND ATG4A ELISA kits, Cysteine protease ATG4A Proteins
Many biological assays use antibodies to detect Cysteine protease ATG4A, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Cysteine protease ATG4A in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Cysteine protease ATG4A antibodies...
Designing a Western blot for Cysteine protease ATG4A? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the ATG4A Western Blot GuideDesigning an IHC experiment for Cysteine protease ATG4A? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the ATG4A IHC GuideATG4A Infographic by Boster Bio
All ATG4A Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
ELISA, Flow Cytometry, WB
$370
Cat # A06539-3
100 μg/vial
Cat # A06539-3
100 μg/vial
Human, Mouse, Rat
ELISA, IHC-P, WB
$481
Cat # A06539
0.1 mg
Cat # A06539
0.1 mg
ATG4A Overview
Facts about Cysteine protease ATG4A.
Cysteine protease ATG4A (ATG4A)
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine.
Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation into phosphatidylethanolamine (PE) and insertion to membranes, which is essential for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.
Gene Information
| Human | |
|---|---|
| Gene Name: | ATG4A |
| Uniprot: | Q8WYN0 |
| Entrez: | 115201 |
Family
| Belongs to: |
|---|
| peptidase C54 family |
Alternative Names
APG4 autophagy 4 homolog A (S. cerevisiae); Apg4a; ATG4 autophagy related 4 homolog A (S. cerevisiae); ATG4A; Atg4al; Autl2; AUT-like 2 cysteine endopeptidase; AUT-like 2, cysteine endopeptidase (S. cerevisiae); Autophagin 2; autophagin-2; cysteine endopeptidase; cysteine protease ATG4A; EC 3.4.22; EC 3.4.22.-; hAPG4A
Molecular Weight
Mass (kDA):
45.378 kDA
Genomic Context
| Human | |
|---|---|
| Location: | Xq22.3 |
| Sequence: | X; NC_000023.11 (108091667..108154671) |
Tissue Specificity
Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.
Subcellular Localizations
Cytoplasm.
Diseases
- Malignant Neoplasms
- Carcinoma
- Neoplasms
- Granuloma
- Crohn Disease
- Neoplasm Metastasis
- Diabetic Ketoacidosis
- Neurodegenerative Disorders
- Malignant Neoplasm Of Breast
- Ataxia
- Intestinal Diseases
- Endoplasmic Reticulum Stress
Interacting Proteins
- CDKL3
- GABARAPL1
Pathways
- Autophagy
- Cell Death
- Macroautophagy
- Pathogenesis
- Granuloma Formation
- Transport
- Programmed Cell Death
- Autophagic Cell Death
- Angiogenesis
PTMs
- Cleavage
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ATG4A
bosterbio.com/bosterbio-gene-info-cards/ATG4A
References
PMID: 12446702 by Marino G., et al. Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy.
PMID: 15169837 by Kabeya Y., et al. LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation.