Anti-Cysteine protease ATG4A ATG4A Antibody

Product Name

Anti-Cysteine protease ATG4A ATG4A Antibody

View all ATG4A Antibodies

SKU/Catalog Number

A06539

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Cysteine protease ATG4A ATG4A Antibody (Catalog # A06539). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ATG4A antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Cysteine protease ATG4A ATG4A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06539)

Host

Rabbit

Contents

ATG4A antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ATG4A antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ATG4A. The immunogen is located within the last 50 amino acids of ATG4A.

Cross-reactivity

ATG4A antibody is human, mouse and rat reactive. At least two isoforms of ATG4A are known to exist; this antibody will detect both isoforms. ATG4A is predicted to not cross-react with other ATG4 proteins.

Reactive Species

A06539 is reactive to ATG4A in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

45.4 kDa

Background of ATG4A

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4A, also known as AUTL2, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3). ATG4 is required for ATG8 conjugation to phosphatidylethanolamine on autophagosomal membranes. In mammals, each ATG4 homolog shows a selective preference for the ATG8 homologs (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06539 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ATG4A antibody can be used for detection of ATG4A by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ATG4A

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