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- Table of Contents
Designing a Western blot for ATG9A? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the ATG9A Western Blot GuideFacts about Autophagy-related protein 9A.
Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes.
| Human | |
|---|---|
| Gene Name: | ATG9A |
| Uniprot: | Q7Z3C6 |
| Entrez: | 79065 |

| Belongs to: |
|---|
| ATG9 family |

APG9 autophagy 9-like 1 (S. cerevisiae); APG9 autophagy 9-like 1; APG9L1APG9-like 1; ATG9 autophagy related 9 homolog A (S. cerevisiae); autophagy 9-like 1 protein; autophagy-related protein 9A; FLJ22169; mATG9; MGD3208
Mass (kDA):
94.447 kDA

| Human | |
|---|---|
| Location: | 2q35 |
| Sequence: | 2; NC_000002.12 (219219380..219229636, complement) |
Cytoplasmic vesicle, autophagosome membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Under amino acid starvation or rapamycin treatment, redistributes from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool. The starvation-induced redistribution depends on ULK1, ATG13, as well as SH3GLB1.






PMID: 15755735 by Yamada T., et al. Endothelial nitric-oxide synthase antisense (NOS3AS) gene encodes an autophagy-related protein (APG9-like2) highly expressed in trophoblast.
PMID: 16940348 by Young A.R., et al. Starvation and ULK1-dependent cycling of mammalian Atg9 between the TGN and endosomes.