Anti-Autophagy-related protein 9A ATG9A Antibody

Product Name

Anti-Autophagy-related protein 9A ATG9A Antibody

View all ATG9A Antibodies

SKU/Catalog Number

A03757

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Autophagy-related protein 9A ATG9A Antibody (Catalog # A03757). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ATG9A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Autophagy-related protein 9A ATG9A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03757)

Host

Rabbit

Contents

ATG9A Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ATG9A antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ATG9A. The immunogen is located within amino acids 720 - 770 of ATG9A.

Reactive Species

A03757 is reactive to ATG9A in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

94.4 kDa

Background of ATG9A

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG9A, a multi-spanning membrane protein localizing to the Golgi apparatus and late endosomes, has been proposed to mediate membrane transport to generate autophagosomes. ATG9A has also been implicated as a regulator of STING (stimulator of interferon genes)-mediated innate immune response.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03757 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ATG9A antibody can be used for detection of ATG9A by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ATG9A

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