Product Info Summary
| SKU: | PA1425 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Carbonic Anhydrase I/CA1 Antibody Picoband®
SKU/Catalog Number
PA1425
BA4061-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Carbonic Anhydrase I/CA1 Antibody catalog # PA1425. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Carbonic Anhydrase I/CA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1425)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Carbonic Anhydrase I, different from the mouse sequence by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1425 is reactive to CA1 in Human, Rat
Observed Molecular Weight
29 kDa
Calculated molecular weight
28.9 kDa
Background of CA1
Carbonic anhydrase 1 is an enzyme that in humans is encoded by the CA1 gene. It is a member of the Carbonic anhydrase. The CA1 gene is mapped to 8q22. CAI has got about 260 amino acids. This protein is highly expressed in erythrocytes. As catalysts of the reversible hydration of carbon dioxide, CAI participate in a variety of biologic processes like respiration, calcification, acid-base balance etc.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1425 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: human HEL whole cell, rat spleen tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (PA1425).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: rat spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Carbonic Anhydrase I/CA1 antigen affinity purified polyclonal antibody (Catalog # PA1425) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Carbonic Anhydrase I/CA1 at approximately 29 kDa. The expected band size for Carbonic Anhydrase I/CA1 is at 29 kDa.
Specific Publications For Anti-Carbonic Anhydrase I/CA1 Antibody Picoband® (PA1425)
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7 Customer Q&As for Anti-Carbonic Anhydrase I/CA1 Antibody Picoband®
Question
Does PA1425 anti-Carbonic Anhydrase I/CA1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
You can see on the product datasheet, PA1425 anti-Carbonic Anhydrase I/CA1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-04
Question
Will anti-Carbonic Anhydrase I/CA1 antibody PA1425 work for WB with pancreas spleen?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
According to the expression profile of pancreas spleen, CA1 is highly expressed in pancreas spleen. So, it is likely that anti-Carbonic Anhydrase I/CA1 antibody PA1425 will work for WB with pancreas spleen.
Boster Scientific Support
Answered: 2019-11-28
Question
Is this PA1425 anti-Carbonic Anhydrase I/CA1 antibody reactive to the isotypes of CA1?
A. Anderson
Verified customer
Asked: 2019-11-18
Answer
The immunogen of PA1425 anti-Carbonic Anhydrase I/CA1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Carbonic Anhydrase I(51-68aa SYNPATAKEIINVGHSFH), different from the mouse sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-18
Question
Is a blocking peptide available for product anti-Carbonic Anhydrase I/CA1 antibody (PA1425)?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
We do provide the blocking peptide for product anti-Carbonic Anhydrase I/CA1 antibody (PA1425). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-11
Question
I was wanting to use your anti-Carbonic Anhydrase I/CA1 antibody for WB for rat pancreas spleen on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat pancreas spleen identification?
Verified Customer
Verified customer
Asked: 2018-03-08
Answer
You can see on the product datasheet, PA1425 anti-Carbonic Anhydrase I/CA1 antibody has been tested for WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat pancreas spleen in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-08
Question
Is there a BSA free version of anti-Carbonic Anhydrase I/CA1 antibody PA1425 available?
Verified Customer
Verified customer
Asked: 2017-06-12
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Carbonic Anhydrase I/CA1 antibody PA1425 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-06-12
Question
Will anti-Carbonic Anhydrase I/CA1 antibody PA1425 work on pig WB with liver?
S. Wu
Verified customer
Asked: 2013-04-23
Answer
Our lab technicians have not tested anti-Carbonic Anhydrase I/CA1 antibody PA1425 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Carbonic Anhydrase I/CA1 antibody PA1425 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-04-23


