Anti-Carbonic Anhydrase I/CA1 Antibody Picoband®

CA1 antibody

Boster Bio Anti-Carbonic Anhydrase I/CA1 Antibody catalog # PA1425. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PA1425
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Carbonic Anhydrase I/CA1 Antibody Picoband®

View all CA1 Antibodies

SKU/Catalog Number

PA1425
BA4061-2 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Carbonic Anhydrase I/CA1 Antibody catalog # PA1425. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Carbonic Anhydrase I/CA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1425)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Carbonic Anhydrase I, different from the mouse sequence by one amino acid.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1425 is reactive to CA1 in Human, Rat

Observed Molecular Weight

29 kDa

Calculated molecular weight

28.9 kDa

Background of CA1

Carbonic anhydrase 1 is an enzyme that in humans is encoded by the CA1 gene. It is a member of the Carbonic anhydrase. The CA1 gene is mapped to 8q22. CAI has got about 260 amino acids. This protein is highly expressed in erythrocytes. As catalysts of the reversible hydration of carbon dioxide, CAI participate in a variety of biologic processes like respiration, calcification, acid-base balance etc.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1425 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Positive Control

WB: human HEL whole cell, rat spleen tissue

Validation Images & Assay Conditions

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7 Customer Q&As for Anti-Carbonic Anhydrase I/CA1 Antibody Picoband®

Question

Does PA1425 anti-Carbonic Anhydrase I/CA1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-04

Answer

You can see on the product datasheet, PA1425 anti-Carbonic Anhydrase I/CA1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-04

Question

Will anti-Carbonic Anhydrase I/CA1 antibody PA1425 work for WB with pancreas spleen?

Verified Customer

Verified customer

Asked: 2019-11-28

Answer

According to the expression profile of pancreas spleen, CA1 is highly expressed in pancreas spleen. So, it is likely that anti-Carbonic Anhydrase I/CA1 antibody PA1425 will work for WB with pancreas spleen.

Boster Scientific Support

Answered: 2019-11-28

Question

Is this PA1425 anti-Carbonic Anhydrase I/CA1 antibody reactive to the isotypes of CA1?

A. Anderson

Verified customer

Asked: 2019-11-18

Answer

The immunogen of PA1425 anti-Carbonic Anhydrase I/CA1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Carbonic Anhydrase I(51-68aa SYNPATAKEIINVGHSFH), different from the mouse sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-18

Question

Is a blocking peptide available for product anti-Carbonic Anhydrase I/CA1 antibody (PA1425)?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

We do provide the blocking peptide for product anti-Carbonic Anhydrase I/CA1 antibody (PA1425). If you would like to place an order for it please contact support@bosterbio.com and make a special request.

Boster Scientific Support

Answered: 2019-06-11

Question

I was wanting to use your anti-Carbonic Anhydrase I/CA1 antibody for WB for rat pancreas spleen on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat pancreas spleen identification?

Verified Customer

Verified customer

Asked: 2018-03-08

Answer

You can see on the product datasheet, PA1425 anti-Carbonic Anhydrase I/CA1 antibody has been tested for WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat pancreas spleen in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-03-08

Question

Is there a BSA free version of anti-Carbonic Anhydrase I/CA1 antibody PA1425 available?

Verified Customer

Verified customer

Asked: 2017-06-12

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Carbonic Anhydrase I/CA1 antibody PA1425 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-06-12

Question

Will anti-Carbonic Anhydrase I/CA1 antibody PA1425 work on pig WB with liver?

S. Wu

Verified customer

Asked: 2013-04-23

Answer

Our lab technicians have not tested anti-Carbonic Anhydrase I/CA1 antibody PA1425 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Carbonic Anhydrase I/CA1 antibody PA1425 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-04-23

Order DetailsPrice
PA1425

100μg

$370
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PA1425-Biotin

100 μg Biotin conjugated (liquid)

$570
PA1425-HRP

100 μg HRP conjugated (liquid)

$570
PA1425-PE

100 μg PE conjugated (liquid)

$820
PA1425-carrier-free

Carrier Free

$370
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PA1425
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Apr 12, 2026