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CALB2 / Calretinin · IHC design guide
Design Immunohistochemistry for CALB2
Plan CALB2 chromogenic IHC on paraffin sections with tissue controls and consistent fixation. Interpret cytoplasmic staining by cell type, including brain cells and testicular Leydig cells (HPA tissue IHC).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
CALB2 Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Cytoplasmic staining (HPA tissue IHC); dendrites and synapses (UniProt) | |
| Staining pattern | Cytoplasmic signal in brain cells and Leydig cells (HPA tissue IHC) | |
| Antigen retrieval | EDTA pH 8.0 HIER, heat-mediated (datasheet A04255) | |
| Positive control | Adrenal gland+4 more · see all | |
| Negative control | Appendix+4 more · see all |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Keep fixation consistent (standard IHC practice; not target-specific); Matched tissue-IHC evidence does not establish the fixation claim. Validate the specimen-specific method before use. (selected-SKU IHC image A04255) | |
| Caveat | Staining varies by cell type and tissue (HPA tissue IHC) | |
| Regulation | RNA enhanced in adipose and brain (HPA tissue RNA) | |
| Isoform / epitope | No isoforms; one 1–271 chain, with no extracellular domain (UniProt) |
Section 1
Recommended CALB2 IHC & IF Protocols
The catalog antibody protocol (datasheet A04255) is followed by three published chromogenic IHC protocols for CALB2 in gonadal tissue and medulla (PMC7552167; PMC7729007; PMC12567713).
| Sample | Paraffin-embedded human rectal cancer tissue; fixative not specified (datasheet A04255) |
| Fixation | Image fixative and duration unreported (datasheet A04255); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat retrieval: EDTA pH 8.0 (datasheet A04255); 20 min, 95–100 °C (standard) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% goat serum (datasheet A04255) |
| Primary antibody | Rabbit anti-CALB2, 0.5-1μg/ml (datasheet A04255) |
| Primary incubation | Overnight at 4 °C (datasheet A04255) |
| Detection | Streptavidin-biotin complex (SABC), DAB chromogen (datasheet A04255) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | CALB2-positive staining in glandular cells of adrenal gland (HPA tissue IHC: High). HPA tissue profile: Cytoplasmic expression in several tissues and cells, including brain, adipose tissue, fallopian tube, retina, endocrine pancreas and Leydig cells. No signal in the no-primary control. |
💡Decision noteStart with heat-mediated EDTA retrieval at pH 8.0 (datasheet A04255). A published medulla protocol also uses high-pH pretreatment (PMC7729007).
Section 2
What Is the Expected CALB2 Staining Pattern?
CALB2 staining should be predominantly cytoplasmic in selected cells, including neuronal cells and Leydig cells (HPA tissue IHC: High in caudate and cortical neuronal cells; High in Leydig cells). Signal may extend into neuronal projections (HPA tissue IHC: High in hypothalamic neuronal projections; UniProt P22676: dendrite and synapse). CALB2 has no transmembrane segment (UniProt P22676 topology). The tissue IHC profile has Enhanced reliability, reflecting high agreement between staining and RNA expression (HPA tissue IHC: Enhanced).
| Defined cytoplasmic staining in caudate or cortical neuronal cells, with stained processes where visible (HPA tissue IHC: High in both neuronal populations; High in hypothalamic projections). | This fits the expected cellular distribution (HPA tissue IHC: cytoplasmic profile; UniProt P22676: dendrite and synapse). Assess recognizable cells and processes rather than judging an entire brain section uniformly positive (HPA tissue IHC: cell-specific observations). |
| Predominantly nuclear staining, especially without accompanying cytoplasmic signal. | An isolated nuclear pattern lacks support from the supplied localization records (HPA subcellular: supported cytosol; UniProt P22676: dendrite and synapse). Treat it as suspect and compare it with a known-positive section and detection controls before interpreting it as CALB2 (general IHC practice). |
| Strong staining in appendix glandular cells or bone-marrow hematopoietic cells. | Those particular cell populations are recorded as Not detected (HPA tissue IHC: appendix glandular cells; bone-marrow hematopoietic cells). Investigate antibody cross-reactivity or detection background (general IHC practice). Do not generalize this finding to every glandular cell: adrenal glandular cells are High (HPA tissue IHC). |
| Diffuse color across cells, stroma, and section edges, obscuring cell boundaries. | A broad haze cannot establish the cell-restricted CALB2 pattern (HPA tissue IHC: cell-specific staining). It can arise from nonspecific binding, incomplete blocking, or detection background (general IHC practice); interpret only after the background is reduced and positive cells remain distinct. |
| No signal in morphologically identifiable Leydig cells or caudate neuronal cells. | These are reported High populations (HPA tissue IHC: testis Leydig cells; caudate neuronal cells). A blank result there raises a workflow or reagent question (general IHC practice), but one negative section alone cannot establish absent CALB2 expression. |
💡Expected CALB2 appearanceCall a result positive when distinct cytoplasmic staining marks the expected cells, potentially including neuronal processes, with strong staining in an HPA High population; isolated nuclear signal or widespread color in HPA Not detected cell populations is suspect (HPA tissue IHC; HPA subcellular: supported cytosol; UniProt P22676: dendrite and synapse).
| Compartment and topology (HPA subcellular: supported cytosol; UniProt P22676 topology). | CALB2 has no transmembrane segment, and its supported cellular location is cytosolic (UniProt P22676 topology; HPA subcellular). Expect intracellular staining; a continuous cell-surface rim alone is insufficient, although cerebellar GLUC cells are annotated as cytoplasm/membrane (HPA tissue IHC). |
| Choice of comparison cells (HPA tissue IHC). | Use a reported High cell population as the positive reference and a specified Not detected population as a comparison (HPA tissue IHC: caudate neuronal cells High; appendix glandular cells Not detected). Low staining in lung macrophages is recorded, so faint macrophage signal is not automatically abnormal (HPA tissue IHC: Low). |
| Antibody validation (HPA antibodies: IHC Enhanced). | HPA007305, HPA007306, and CAB000106 each have Enhanced IHC status (HPA antibodies). That status supports the reported IHC pattern through agreement between independent antibodies or orthogonal data; it does not validate an unexpected pattern in every specimen (HPA antibody-validation definition; general IHC interpretation). |
| Antigen retrieval and fixation (general IHC practice). | The supplied UniProt and HPA records provide no CALB2-specific retrieval condition or fixation-sensitivity result. Compare routine paraffin-section retrieval conditions only if the known-positive control fails, and judge them by specific signal versus background (general IHC practice). |
| Protein form and epitope limits (UniProt P22676). | The record lists one chain spanning residues 1–271, no signal peptide or propeptide, and no annotated isoforms (UniProt P22676 processing). It also lists EF-hand domains and phosphotyrosine at residue 214; without an antibody epitope map, these details do not predict retrieval needs or a staining difference (UniProt P22676; general IHC interpretation). |
| Situation | Likely cause | Next action |
|---|---|---|
| A known-positive section is blank (HPA tissue IHC: caudate neuronal cells High). | The target cells may be absent from the examined field, or a staining step may have failed (general IHC practice). | Verify the cell population on the section, then check antibody application, retrieval, detection reagents, and counterstain against a working positive control (general IHC practice). |
| Nuclei stain more strongly than cytoplasm. | Predominantly nuclear localization conflicts with the supported cytosolic location (HPA subcellular: supported cytosol); nonspecific binding or detection background is possible (general IHC practice). | Compare the same run with a known-positive section and a primary-antibody omission control; adjust blocking or antibody concentration if background persists (general IHC practice). |
| Appendix glandular cells show strong signal (HPA tissue IHC: Not detected). | The pattern may reflect cross-reactivity or endogenous detection activity rather than CALB2 (general IHC practice; HPA tissue IHC: appendix glandular cells Not detected). | Check a primary-antibody omission control and the detection-system control, then confirm that an expected High cell population still stains specifically (general IHC practice; HPA tissue IHC). |
| Color is diffuse throughout the section. | Nonspecific binding or excessive chromogen development can obscure cell boundaries (general IHC practice). | Review blocking, washes, antibody concentration, and development time; retain conditions that resolve individual positive cells with a clear background (general IHC practice; HPA tissue IHC: cell-specific profile). |
| Only faint signal appears in lung macrophages. | Low staining is reported for this population (HPA tissue IHC: lung macrophages Low), so intensity alone cannot establish a failed assay. | Judge the run using a reported High population and a specified Not detected population; avoid treating the whole lung as a negative control (HPA tissue IHC; general IHC practice). |
| IF/ICC Q: Where should CALB2 signal appear? | A: Cytosol is the supported ICC-IF location (HPA subcellular: supported cytosol). | Interpret IF/ICC on its own guide page; compare localization with the supported cytosolic record, while using this page's cell-specific tissue observations only for IHC interpretation (HPA subcellular; HPA tissue IHC). |
Experiment evidence
IHC & IF images showing CALB2 staining
A04255 · IHCIHC analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (A04255). Calretinin/CALB2 was detected in paraffin-embedded section o
A04255 · IHCIHC analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (A04255). Calretinin/CALB2 was detected in paraffin-embedded section o
A04255 · IFIF analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (A00346). Calretinin/CALB2 was detected in immunocytochemical section o
PA1015 · IHCIHC analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (PA1015). Calretinin/CALB2 was detected in a paraffin-embedded section
PA1015 · IHCIHC analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (PA1015). Calretinin/CALB2 was detected in a paraffin-embedded section
PA1015 · IFIF analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (PA1015). Calretinin/CALB2 was detected in a paraffin-embedded section
PB9248 · IHCAnti-Calretinin vendor antibody, PB9248, IHC(P) IHC(P): Mouse Brain Tissue
PB9248 · IHCAnti-Calretinin vendor antibody, PB9248, IHC(P) IHC(P): Rat Brain Tissue
HPA · HypothalamusHuman Protein Atlas IHC, Hypothalamus. Source: https://www.proteinatlas.org/ENSG00000172137-CALB2/tissue/hypothalamus#imid_4374
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000172137-CALB2/tissue/cerebral+cortex#imid_2065368
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000172137-CALB2/tissue/colon#imid_2065294
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000172137-CALB2/tissue/liver#imid_2065295
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody HPA007305; green = CALB2, blue = nucleus, red = microtubules.
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody HPA007305; green = CALB2, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for CALB2 IHC & IF
🧪Run caudate first: neuronal cells should stain (HPA: High in caudate neuronal cells). Use appendix glandular cells as the negative tissue (HPA: Not detected in appendix glandular cells); on the caudate slide, cells outside the expected neuronal staining pattern should show background-level signal.
Positive control tissue: Adrenal gland (Glandular cells, HPA High)
Negative control tissue: Appendix (HPA Not detected)
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show CALB2 in A-431, AF22, U2OS, U-251MG, NIH 3T3, with annotated localisation: Cytosol (supported) (HPA subcellular).
Technical controls: Include a no-primary, secondary-only control and a host- and clonality-matched rabbit IgG isotype control (caption: rabbit primary antibody); confirm specificity with a CALB2 knockout specimen or a validated peptide-block control. For caudate, check endogenous peroxidase and biotin background when using the caption’s SABC/DAB detection (caption: SABC with DAB).
⚠️Feasibility: A target-specific fixation window or fixation effect is unreported, and the selected A04255 paraffin-section caption does not state a fixative. The caption uses heat retrieval in EDTA at pH 8.0, but whether CALB2 staining depends on retrieval is unreported (caption: EDTA retrieval). No supplied evidence establishes that frozen sections or IF/ICC are easier; if using IF in brain tissue, assess autofluorescence, including lipofuscin, alongside the negative controls.
HPA tissue IHC evidence for CALB2
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Enhanced — High consistency between antibody staining and RNA expression data.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | Glandular cells | High | Protein (IHC) | HPA → |
| Caudate | Neuronal cells | High | Protein (IHC) | HPA → |
| Cerebellum | GLUC cells - cytoplasm/membrane | High | Protein (IHC) | HPA → |
| Cerebral cortex | Neuronal cells | High | Protein (IHC) | HPA → |
| Hippocampus | Glial cells | High | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Appendix | Glandular cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | Hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Bronchus | Basal cells | Not detected | Protein (IHC) | HPA → |
| Cervix | Glandular cells | Not detected | Protein (IHC) | HPA → |
| Duodenum | Glandular cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced CALB2 IHC Tips
Troubleshoot CALB2 staining in paraffin sections by checking retrieval, controls, cellular location and scoring before interpreting chromogenic signal.
How should I adjust retrieval when CALB2 staining is weak or uneven?
Start with heat-mediated retrieval in EDTA at pH 8.0 for paraffin sections (datasheet A04255). The selected tissue image used this retrieval before incubation with 1 µg/ml catalog antibody overnight at 4°C (caption A04255). If staining is weak, check that sections were fully deparaffinised and that the heated buffer covered them throughout retrieval (standard IHC practice). Compare any change in heating time on adjacent sections alongside the original condition and a positive control (standard IHC practice). Record whether the change improves staining in expected cells without increasing diffuse background (standard IHC practice).
Could fixation explain weak CALB2 staining in my paraffin sections?
The selected paraffin-section caption does not state a fixative, so CALB2-specific sensitivity to fixation is unknown (caption A04255). Record the fixative, fixation duration and processing history for each specimen before comparing staining across cases (standard IHC practice). If weak signal tracks with one processing batch, stain a known positive section in the same run and compare adjacent sections processed under documented conditions (standard IHC practice). Keep retrieval and the catalog antibody concentration at 1 µg/ml constant during that comparison (caption A04255; standard IHC practice). Do not infer fixation tolerance from CALB2 tissue-expression patterns or its protein features (HPA tissue IHC; UniProt P22676).
Where should CALB2 signal appear, and when is its location suspicious?
Evaluate CALB2 principally as cellular cytoplasmic staining: tissue IHC reports cytoplasmic expression, and subcellular imaging supports cytosolic localisation (HPA tissue IHC; HPA subcellular). CALB2 is also annotated at synapses and dendrites, including dendrioles of unipolar brush cells (UniProt P22676 localisation). In sections with neuronal processes, compare process staining with nearby cellular staining and tissue morphology before assigning it to CALB2 (UniProt P22676 localisation; standard IHC practice). Isolated staining confined to section edges or damaged areas warrants review against a matched negative control (standard IHC practice). A purely membrane-rim pattern needs additional validation because CALB2 has no transmembrane segment (UniProt P22676 topology).
How can epitope accessibility affect CALB2 IHC if no isoforms are annotated?
The supplied record lists 0 isoforms and a single CALB2 chain spanning residues 1–271 (UniProt P22676). It also lists 6 EF-hand domains and phosphotyrosine at residue 214, but supplies no antibody epitope location (UniProt P22676; caption A04255). Do not assign a staining difference to a particular domain or modification without epitope-mapping evidence (standard IHC interpretation). Compare adjacent sections under the documented EDTA pH 8.0 retrieval condition while holding detection and exposure to chromogen consistent (datasheet A04255; standard IHC practice). If staining remains discordant with an expected positive control, seek independent specificity evidence before calling an epitope effect (standard IHC practice).
How should I adapt CALB2 localisation checks for a multiplex IF experiment?
For the separate IF/ICC workflow, multiplex CALB2 with a marker of the expected cell population so that signal can be assigned to cells and processes (UniProt P22676 localisation; standard IF practice). Select fluorophores after inspecting the specimen’s autofluorescence in unstained controls, and place the weaker signal in a cleaner detection channel (standard IF practice). Because CALB2 is supported in the cytosol and has no transmembrane segment, use permeabilisation appropriate for an intracellular epitope (HPA subcellular; UniProt P22676 topology; standard IF practice). Check single-stain controls for channel bleed-through and secondary-antibody background (standard IF practice). The supplied IHC caption provides no IF-specific fixation or antibody concentration, so optimise those variables within the IF workflow (caption A04255; standard IF practice).
What should I check when DAB obscures specific CALB2 staining?
First compare a no-primary control with the test section to locate detection-system background (standard IHC practice). The selected image used 10% goat serum blocking, a biotinylated secondary antibody, a streptavidin–biotin complex and DAB (caption A04255). For that workflow, check peroxidase blocking and possible endogenous biotin contribution before increasing the primary-antibody concentration (caption A04255; standard IHC practice). Inspect whether diffuse colour follows tissue folds, edges or damaged areas rather than intact cells (standard IHC practice). Reassess washing and DAB development on adjacent sections while retaining an expected positive tissue control (standard IHC practice).
How should I score CALB2 staining across sections with mixed cell populations? ⚠ ANSWER MARKED FOR VERIFICATION
Define the cell population and anatomic region before scoring, then record the percentage of CALB2-positive cells and staining intensity separately (standard IHC practice). An H-score can combine intensity categories with their positive-cell percentages; state the categories and threshold used (standard IHC practice). Where neuronal processes dominate, report positive-process density per mm² in a defined region instead of treating every profile as a cell (UniProt P22676 localisation; standard IHC practice). Normalise counts to the eligible cells or measured tissue area, excluding folds and necrosis by a prespecified rule (standard IHC practice). Keep retrieval, DAB development and image-acquisition settings consistent across compared sections (datasheet A04255; standard IHC practice).
How can I distinguish true CALB2 positivity from staining artefacts?
A credible result follows intact cellular morphology and the expected cytoplasmic or neuronal-process distribution (HPA tissue IHC; HPA subcellular; UniProt P22676 localisation). Compare cell identity with tissue context: HPA reports high staining in caudate neuronal cells and testis Leydig cells, while appendix glandular cells are reported as not detected (HPA tissue IHC). Treat edge-only colour, necrotic deposits and signal also present in a no-primary control as possible artefacts (standard IHC practice). Check peroxidase blocking when unexplained DAB colour persists, because the selected detection method uses DAB (caption A04255; standard IHC practice). Confirm an unexpected cell type or compartment with an independent control before interpreting it biologically (standard IHC practice).
Boster reagents
Best CALB2 / Calretinin IHC Antibodies
The catalog antibodies have paraffin-section IHC images from human cancer tissue and mouse or rat brain (A04255, PA1015, PB9248: IHC captions); PA1015 also has a mouse-brain IF image (PA1015: IF caption).
Real IHC data
Anti-Calretinin/CALB2 Antibody ®
Cat # A04255
Real IHC data
Anti-Calretinin/CALB2 Antibody ®
Cat # PA1015
Real IHC data
Anti-Calretinin/CALB2 Antibody ®
Cat # PB9248
A04255 has IHC images from human rectal cancer and brain and mouse and rat brain; PA1015 has IHC images from human rectal cancer and mouse and rat brain, plus IF from mouse brain (A04255, PA1015: image captions). PB9248 has IHC images from mouse and rat brain and human intestinal cancer (PB9248: IHC captions).
Which to pick: For paraffin-section chromogenic IHC, A04255 and PA1015 have captions documenting EDTA retrieval at pH 8.0, goat-serum blocking and DAB detection; PB9248 also has paraffin IHC images, but its captions give fewer processing details (A04255, PA1015, PB9248: IHC captions). For IF, choose PA1015 because its own caption shows mouse-brain IF; for ICC, A04255 lists ICC as an application, but its supplied IF caption names a different SKU (PA1015: IF caption; A04255: applications and IF caption). For cross-species tissue IHC, A04255 and PA1015 each show human, mouse and rat samples (A04255, PA1015: IHC captions); the captions do not report the fixative (A04255, PA1015: IHC captions).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry P22676 (CALB2_HUMAN, Calretinin).
- Human Protein Atlas. CALB2 tissue IHC expression (reliability: Enhanced).
- Human Protein Atlas. CALB2 subcellular location (ICC-IF): Localized to the cytosol..
- Human Protein Atlas. CALB2 antibody validation summary (3 antibodies).
- Characterization and Localization of Calb2 in Both the Testis and Ovary of the Japanese Flounder (Paralichthys olivaceus). Animals : an open access journal from MDPI 2020 — PMC7552167.
- Hypertrophy of the Anterior External Arcuate Fasciculus: A Rare Variant With Implications for the Development of the Arcuate Nucleus. Frontiers in neuroanatomy 2020 — PMC7729007.
- Unraveling Spermatogenesis in Molly Fish (Poecilia sphenops): An Integrative Study of Testicular Ultrastructure and Immunohistochemistry. Veterinary sciences 2025 — PMC12567713.
- RNA-programmable cell type monitoring and manipulation in the human cortex with CellREADR. bioRxiv : the preprint server for biology 2025 — PMC11642864.
- PubMed PMID:2618861 — UniProt-cited evidence.
- PubMed PMID:2001709 — UniProt-cited evidence.
- PubMed PMID:14702039 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for CALB2 (Calretinin). Expected localisation Cytoplasmic staining (HPA tissue IHC); dendrites and synapses (UniProt). Antigen retrieval: EDTA pH 8.0 HIER, heat-mediated (datasheet A04255). Validated IHC antibody: A04255 at 0.5-1μg/ml (the product's own IHC validation image is shown). Controls (HPA): positive Adrenal gland (Glandular cells); negative Appendix. IHC-P workflow on paraffin sections; fixative unspecified. Protocol steps: 1. Sample: Paraffin-embedded human rectal cancer tissue; fixative not specified (datasheet A04255); 2. Fixation: Image fixative and duration unreported (datasheet A04255); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat retrieval: EDTA pH 8.0 (datasheet A04255); 20 min, 95–100 °C (standard); 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% goat serum (datasheet A04255); 8. Primary antibody: Rabbit anti-CALB2, 0.5-1μg/ml (datasheet A04255); 9. Primary incubation: Overnight at 4 °C (datasheet A04255). Watch for: Fixation: Keep fixation consistent (standard IHC practice; not target-specific); Matched tissue-IHC evidence does not establish the fixation claim. Validate the specimen-specific method before use. (selected-SKU IHC image A04255); Caveat: Staining varies by cell type and tissue (HPA tissue IHC); Isoform / epitope: No isoforms; one 1–271 chain, with no extracellular domain (UniProt); Regulation: RNA enhanced in adipose and brain (HPA tissue RNA). Evidence: UniProt P22676, HPA tissue IHC · Enhanced, HPA ICC-IF · cytosol., Datasheet A04255, PMC7552167, PMC7729007. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/CALB2/immunohistochemistry-ihc-guide.