Anti-Calretinin/CALB2 Antibody

Calretinin antibody

Boster Bio Anti-Calretinin/CALB2 Antibody catalog # PA1015. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1015
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, WB

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Product Name

Anti-Calretinin/CALB2 Antibody

View all Calretinin Antibodies

SKU/Catalog Number

PA1015

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Calretinin/CALB2 Antibody catalog # PA1015. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Calretinin/CALB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1015)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Calretinin (4-18aa PQQQPPYLHLAELTA), identical to the related rat sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1015 is reactive to CALB2 in Human, Mouse, Rat

Applications

PA1015 is guaranteed for IF, IHC, WB Boster Guarantee

Background of Calretinin

Calbindin is a calcium-binding protein belonging to the troponin C superfamily. Calretinin is expressed in central and peripheral nervous system and in many normal and pathological tissues. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunofluorescence, 5 μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CALB2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P22676

Gene ID

794

Gene Name

CALB2

Full Name

Calretinin

Weight

31.54kDa

Superfamily

calbindin family

Alternative Names

29 kDa calbindin; CAB29; CAL2; CALB2; calbindin 2; calbindin 2, (29kD, calretinin); calbindin 2, 29kDa (calretinin); calbindin D29K; Calretinin; CR CALB2 CAB29, CAL2, CR calbindin 2 calretinin|29 kDa calbindin|calbindin 2, (29kD, calretinin)|calbindin D29K|testicular secretory protein Li 8

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CALB2, check out the CALB2 Infographic

CALB2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CALB2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for PA1015

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6 Customer Q&As for Anti-Calretinin/CALB2 Antibody

Question

Would PA1015 anti-Calretinin/CALB2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

It shows on the product datasheet, PA1015 anti-Calretinin/CALB2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-22

Question

We are interested in to test anti-Calretinin/CALB2 antibody PA1015 on mouse adipose tissue cerebellum for research purposes, then I may be interested in using anti-Calretinin/CALB2 antibody PA1015 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

T. Yang

Verified customer

Asked: 2019-04-18

Answer

The products we sell, including anti-Calretinin/CALB2 antibody PA1015, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-04-18

Question

Does anti-Calretinin/CALB2 antibody PA1015 work on zebrafish IHC with adipose tissue cerebellum?

T. Patel

Verified customer

Asked: 2018-06-05

Answer

Our lab technicians have not tested anti-Calretinin/CALB2 antibody PA1015 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Calretinin/CALB2 antibody PA1015 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish adipose tissue cerebellum in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-06-05

Question

I see that the anti-Calretinin/CALB2 antibody PA1015 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-03-15

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-03-15

Question

Is a blocking peptide available for product anti-Calretinin/CALB2 antibody (PA1015)?

Verified Customer

Verified customer

Asked: 2018-01-15

Answer

We do provide the blocking peptide for product anti-Calretinin/CALB2 antibody (PA1015). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-01-15

Question

Is there a BSA free version of anti-Calretinin/CALB2 antibody PA1015 available?

Verified Customer

Verified customer

Asked: 2017-05-11

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Calretinin/CALB2 antibody PA1015 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-05-11

Size

Total: $315

SKU:PA1015

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PA1015
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

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