CCL17 · Western blot design guide

CCL17 Western Blot Planning Guide

Plan a CCL17 Western blot around the catalog-observed 10.5 kDa band, image-backed PB9031 evidence, HPA controls, and verified protocol records.

Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use

Western blot protocol sheet for CCL17 (CCL17): expected band 10.5 kDa, antibody PB9031, and PMC-cited SDS-PAGE protocol steps

Band Protocols Controls Antibodies

CCL17 Western Blot Experimental Design Guide

Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.

Must know before running

	Expected band	10.5 kDa
	Observed band	Not reported — verify product WB image
	Gel	15%
	Positive control ⓘ	Cerebellum
	Negative control ⓘ	Soft tissue

Important caveats

Reasons your observed band may differ from the expected size.

ⓘ	Calculated mass	10.5 kDa
ⓘ	Localization	Secreted
ⓘ	Processing / PTM	Record-dependent
ⓘ	Reactivity	Mouse

Sample controls for CCL17 Western blot

🧪Use Cerebellum as the first positive-control candidate and Soft tissue as the HPA Not detected negative candidate.

Positive control: Cerebellum (Medium)

Negative control: Soft tissue (Not detected)

HPA protein score determines control status; other expression data is supporting context only.

HPA tissue expression evidence for CCL17

Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.

Positive expression · recommended positive controls

Tissue	Cell type	Level	Evidence	Source
Cerebellum	Reported tissue cells	Medium	Protein (HPA)	HPA →
Breast	Reported tissue cells	Medium	Protein (HPA)	HPA →
Appendix	Reported tissue cells	Medium	Protein (HPA)	HPA →
Bone marrow	Reported tissue cells	Medium	Protein (HPA)	HPA →

Undetected expression · recommended negative controls

Tissue	Cell type	Level	Evidence	Source
Soft tissue	Reported tissue cells	Not detected	Protein (HPA)	HPA →
Adipose tissue	Reported tissue cells	Not detected	Protein (HPA)	HPA →

Section 1

What Is the Expected CCL17 Western Blot Band Size?

Use the product-observed 10.5 kDa band as the primary planning value and retain the UniProt calculated mass as context.

What am I looking at on my blot?
10.5 kDa	Matches the authoritative product WB observation.
10.5 kDa calculated	Use as UniProt context, not as a replacement observed band.
Unexpected additional signal	Do not assign identity without orthogonal positive/negative controls.

💡Expected CCL17 appearancePlan around 10.5 kDa and keep the calculated mass as supporting context.

How each factor affects band size
Catalog-observed band	10.5 kDa; use this as the primary experimental expectation.
Calculated mass	10.5 kDa from UniProt Q92583; retain as context.
Gel selection	15%; shared with the recommended protocol and poster.
Specificity check	Compare the lead HPA positive and negative controls with PB9031.

Why is my band missing or off?
Situation	Likely cause	Next action
10.5 kDa	Matches the authoritative product WB observation.	Confirm with orthogonal controls and the linked product record.
Additional band	May reflect processing, modification, or non-specific signal.	Run a dilution series and compare positive/negative controls.
Weak signal	Target abundance or transfer may be limiting.	Verify transfer, increase positive-control abundance, and bracket exposure.

Sources: UniProt Q92583 Human Protein Atlas product datasheet (PB9031)

Experiment evidence

Western blot images showing CCL17 band

PB9031 · CCL17 — observed 10.5 kDa WB validation

PB9031 · CCL17Observed 10.5 kDa · product-specific WB image

← swipe / scroll for more images →

Real WB data behind the band-size expectations in this section.

Section 2

Real Curated CCL17 Western Blot Protocols

Start with the molecular-weight rule, then compare verified publication-derived conditions.

Recommended Western blot protocol parameters
Sample / lysate	Appendix
Gel %	15%
Load	20-30 µg total protein per lane
Transfer	Semi-dry, short transfer
Membrane	0.2 µm PVDF
Blocking	5% non-fat milk or 5% BSA in TBST
Primary	PB9031 at datasheet starting dilution
Primary incubation	Overnight at 4 °C with gentle agitation
Secondary	Species-matched HRP conjugate at validated dilution
Wash	3 × 5 min in TBST
Detection	Chemiluminescent substrate
Exposure	Bracket exposures to avoid saturation

Section 4

Advanced CCL17 Western Blot Tips

Deeper troubleshooting and optimisation questions for CCL17, answered from its protein features.

Which band should guide the blot?

Use 10.5 kDa, the observation attached to the authoritative PB9031 WB record.

How should calculated mass be interpreted?

Treat the UniProt calculated mass as context; it does not replace the catalog-observed 10.5 kDa expectation.

Which positive control should I start with?

Start with Cerebellum, the lead HPA protein-expression candidate.

Which negative control is defensible?

Use Soft tissue as an orthogonal HPA Not detected candidate.

Which gel should I use?

Use 15% consistently across the quick facts, protocol table, and poster.

What transfer method to use for CCL17 Western blot?

Use the transfer method in the recommended protocol and verify transfer before blocking.

How should PB9031 be started?

Start at the linked datasheet condition and run a three-point primary-antibody dilution test.

Which publication-derived protocols can I compare?

Compare only the supplied target-verified records: PMC6689663, PMC12020124, PMC5945007.

Boster reagents

CCL17 Western Blot Reagents

Mouse-reactive CCL17 Western blot reagents with authoritative product imagery.

Real WB data Western blot validation image for CCL17 using PB9031; observed band 10.5 kDa

Anti-TARC/CCL17 Antibody Picoband®

Cat # PB9031

Reactivity: Mouse

View product →

Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.

Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.