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- Table of Contents
CDR1 / Cerebellar degeneration-related antigen 1 · Western blot design guide
Design a Western Blot for CDR1
Real validated CDR1 Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-CDR1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
CDR1 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~31.3 kDa | |
| Observed band | ~31 kDa | |
| Gel | 5–20% (catalog A08472-1) | |
| Positive control | Epididymis (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | — | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Real Curated CDR1 Western Blot Protocols
The A08472-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human U-87MG, mouse RAW264.7 (catalog A08472-1) |
| Gel % | 5–20% (catalog A08472-1) |
| Load | 30 ug; reducing conditions (catalog A08472-1) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A08472-1) |
| Membrane | nitrocellulose membrane (catalog A08472-1) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A08472-1) |
| Primary antibody | A08472-1 · 0.5 μg/mL (catalog A08472-1) |
| Primary incubation | overnight at 4°C (catalog A08472-1) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A08472-1) |
| Secondary incubation | 1.5 hour at RT (catalog A08472-1) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A08472-1) |
| Detection | ECL (catalog A08472-1) |
Section 2
What Is the Expected CDR1 Western Blot Band Size?
CDR1 is predicted at 31.3 kDa and observed at ~31 kDa; the small difference has no established cause in the supplied evidence.
| Single band at ~31 kDa | Consistent with the reported CDR1 band |
| Sharp band near 31.3 kDa | Consistent with the UniProt predicted mass |
| Strongest band at ~31 kDa | Matches the reported position; confirm its identity |
| Faint band at ~31 kDa | Matches the reported position; confirm its identity |
💡Expected CDR1 appearanceCDR1 has a predicted mass of 31.3 kDa and a reported band at ~31 kDa; confirm identity with a positive control or a second antibody.
| UniProt predicted mass | Places full-length CDR1 near 31.3 kDa |
| Predicted full-length mass | Provides the reference for assessing the ~31 kDa observed band |
| 31,279 Da calculated mass | Corresponds to approximately 31.3 kDa |
| Predicted mass near 31 kDa | Agrees with the reported ~31 kDa band |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | CDR1 may be below detection in the tested lysate | Compare with U-87MG or RAW264.7 lysate as a positive control |
| Band higher than expected | The band’s identity or migration is unconfirmed | Check molecular weight markers and confirm with a second CDR1 antibody |
| Band lower than expected | The band’s identity is unconfirmed | Compare with a positive control and confirm with a second CDR1 antibody |
| Multiple bands | Additional bands may be nonspecific | Identify the ~31 kDa band using a positive control and a second antibody |
| Weak or no signal | CDR1 signal may be below assay detection | Check transfer and antibody performance with a positive control |
Experiment evidence
Western blot images showing CDR1 band
A08472-1 · CDR1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for CDR1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for CDR1 in Western blot, you can use epididymis tissue, which HPA scores High for expression.
Positive control: Epididymis (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: HPA reports high expression in epididymis and no detection in adipose tissue, providing a feasible tissue pair.
HPA tissue expression evidence for CDR1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Epididymis | Glandular cells | High | Protein (IHC) | HPA → |
| Kidney | Cells in tubules | High | Protein (IHC) | HPA → |
| Appendix | Glandular cells | Medium | Protein (IHC) | HPA → |
| Breast | Glandular cells | Medium | Protein (IHC) | HPA → |
| Duodenum | Glandular cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | Adipocytes | Not detected | Protein (IHC) | HPA → |
| Bone marrow | Hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Caudate | Glial cells | Not detected | Protein (IHC) | HPA → |
| Cerebellum | Cells in granular layer | Not detected | Protein (IHC) | HPA → |
| Cervix | Glandular cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced CDR1 Western Blot Tips
Deeper troubleshooting and optimisation questions for CDR1, answered from its protein features.
Where should the CDR1 band appear?
Band shift · CDR1 has a predicted mass of 31.3 kDa, and the supplied observed band is approximately 31 kDa. Use that region as the starting point for identifying the band.
Could listed isoforms explain multiple bands?
Isoforms · UniProt lists one CDR1 isoform and no alternative sequence. Do not assign additional bands to isoforms based on these features.
Are modification-related band shifts supported?
PTM · No modified residues or glycosylation sites are listed for CDR1. These features provide no basis for assigning a shifted band to a specific modification.
Does this guide establish induction of CDR1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for CDR1?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A08472-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
Which band should be quantified?
Quantitation · Quantify a consistently identified band near 31 kDa, the supplied observed size and close to the 31.3 kDa prediction. Keep any additional bands separate unless their identity is established.
Does the observed band show a meaningful mass shift?
Interpretation · The approximately 31 kDa observed band closely matches the 31.3 kDa prediction. The supplied features do not establish a cause for any small difference between apparent and calculated mass.
Compare them with the expected approximately 31 kDa band. CDR1 is annotated as repeat-containing, but that annotation alone does not identify an unexpected band or explain its size; verify band identity before interpreting it as CDR1.
Compare them with the expected approximately 31 kDa band. CDR1 is annotated as repeat-containing, but that annotation alone does not identify an unexpected band or explain its size; verify band identity before interpreting it as CDR1.
Boster reagents
CDR1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-CDR1 Antibody Picoband®
Cat # A08472-1
The catalog reports one anti-CDR1 antibody for Western blot, A08472-1. Its WB image shows a band near the expected 31 kDa in human U-87MG and mouse RAW264.7 whole cell lysates. No publication or independent validation evidence was supplied.
Which to pick: A08472-1 is the only listed option. It is catalogued as reactive with human and mouse, and its WB image uses 30 µg reducing whole cell lysate per lane and 0.5 µg/mL primary antibody. The pictured cell lines are the tested examples.
Source: BosterBio CDR1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P51861.
- Human Protein Atlas. CDR1 tissue expression.
- PMC5963614 — target-verified WB comparison