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Product Info Summary
|Reactive Species:||Human, Mouse|
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Anti-CDR1 Antibody Picoband™
Boster Bio Anti-CDR1 Antibody Picoband™ catalog # A08472-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CDR1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08472-1)
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
E.coli-derived human CDR1 recombinant protein (Position: M1-R152).
A08472-1 is reactive to CDR1 in Human, Mouse
A08472-1 is guaranteed for ELISA, WB Boster Guarantee
*Blocking peptide can be purchased at $150. Contact us for more information.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CDR1 using anti-CDR1 antibody (A08472-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDR1 antigen affinity purified polyclonal antibody (Catalog # A08472-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDR1 at approximately 31 kDa. The expected band size for CDR1 is at 31 kDa.
Gene/Protein Information For CDR1 (Source: Uniprot.Org, NCBI)
Cerebellar degeneration-related antigen 1
Cerebellar degeneration-related antigen 1 CDR1 CDR, CDR34, CDR62A cerebellar degeneration related protein 1 cerebellar degeneration-related antigen 1|cerebellar degeneration-related protein 1, 34kDa*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CDR1, check out the CDR1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CDR1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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