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- Table of Contents
CELF1 / CUGBP Elav-like family member 1 · Western blot design guide
Design a Western Blot for CELF1
Source-linked CELF1 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-CELF1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
CELF1 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~52.1 kDa | |
| Observed band | ~52 kDa | |
| Gel | 10% (catalog M02163-1) | |
| Positive control | Adrenal gland (IHC candidate; verify WB) +4 more | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Acetylated | |
| Caveat | Phosphorylation-state controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 6 isoform(s) |
Section 1
Source-Linked CELF1 Western Blot Protocol Options
The M02163-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human HeLa, human RT4, human A549, human K562, rat skeletal muscle, rat brain, mouse skeletal muscle, mouse brain (catalog M02163-1) |
| Gel % | 10% (catalog M02163-1) |
| Load | 30 ug; reducing conditions (catalog M02163-1) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog M02163-1) |
| Membrane | nitrocellulose membrane (catalog M02163-1) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog M02163-1) |
| Primary antibody | M02163-1 · 1:500 (catalog M02163-1) |
| Primary incubation | overnight at 4°C (catalog M02163-1) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog M02163-1) |
| Secondary incubation | 1.5 hour at RT (catalog M02163-1) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog M02163-1) |
| Detection | ECL (catalog M02163-1) |
Section 2
What Is the Expected CELF1 Western Blot Band Size?
CELF1 is predicted at 52.1 kDa and observed at ~52 kDa; the cause of their small difference is not established.
| Band at ~52 kDa | Matches the observed CELF1 band and its 52.1 kDa predicted mass. |
| Several bands near ~52 kDa | CELF1 has six named isoforms, but distinct migration has not been established. |
| Band above ~52 kDa | Identity and cause of the higher position require validation. |
| Band below ~52 kDa | Could reflect an isoform or degradation; neither is established by band position alone. |
💡Expected CELF1 appearanceCELF1 is predicted at 52.1 kDa and observed at ~52 kDa in whole-cell and tissue lysates; confirm band identity with a knockdown or independent antibody if the pattern differs.
| Predicted CELF1 mass | The 52.1 kDa prediction closely matches the observed ~52 kDa band. |
| Splice isoform 1 | Its individual mass and migration are not supplied. |
| Splice isoforms 2 and 3 | Their individual masses and any size differences are not supplied. |
| Splice isoforms 4, 5 and 6 | Their individual masses and any size differences are not supplied. |
| Situation | Likely cause | Next action |
|---|---|---|
| Band higher than expected | The higher band has no established feature-based identity. | Check reduction and confirm identity by CELF1 knockdown or an independent antibody. |
| Band lower than expected | An isoform or degradation is possible, but neither is established. | Compare with a knockdown control and prepare lysate with protease inhibitors. |
| Multiple bands | CELF1 has six isoforms, though their migration is not supplied. | Use a knockdown or isoform-specific validation to identify the bands. |
| Weak or no signal | CELF1 abundance in the tested sample may be low. | Check loading and antibody performance with a positive-control lysate. |
| Fragments below expected size | Proteolysis during sample preparation is possible. | Use fresh, chilled lysate with protease inhibitors and confirm fragment identity. |
Experiment evidence
Western blot images showing CELF1 band
M02163-1 · CELF1
A30541 · CELF1
A30541 · CELF1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for CELF1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for CELF1 in Western blot, you can use adrenal gland tissue, which HPA scores High.
Positive control: Adrenal gland (IHC candidate; verify WB)
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside.
⚠️Feasibility: No HPA tissue is undetected, so use siRNA knockdown or a KO line for a negative control.
HPA tissue expression evidence for CELF1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | glandular cells | High | Protein (IHC) | HPA → |
| Appendix | glandular cells | High | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | High | Protein (IHC) | HPA → |
| Breast | glandular cells | High | Protein (IHC) | HPA → |
| Bronchus | respiratory epithelial cells | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Medium | Protein (IHC) | HPA → |
| Heart muscle | cardiomyocytes | Medium | Protein (IHC) | HPA → |
| Liver | cholangiocytes | Medium | Protein (IHC) | HPA → |
| Prostate | glandular cells | Medium | Protein (IHC) | HPA → |
| Seminal vesicle | glandular cells | Medium | Protein (IHC) | HPA → |
Section 3
Advanced CELF1 Western Blot Tips
Deeper troubleshooting and optimisation questions for CELF1, answered from its protein features.
What should be checked if extra CELF1 bands appear?
Band shift · Consider the six listed isoforms and whether the antibody recognizes their differing sequences. Also consider the listed phosphorylation sites, without assuming they cause a visible shift. Band position alone cannot assign an extra band to a particular isoform or modification.
Could CELF1 isoforms produce different bands?
Isoforms · UniProt lists six isoforms with alternative sequences. Isoform 5 lacks residues 1–17; isoforms 2 and 3 lack residues 231–234. These differences could affect apparent size or antibody recognition, but the features do not establish that separate bands will resolve.
That depends on its binding site. Isoform 4 has a changed N terminus; isoform 5 lacks residues 1–17; isoforms 4, 5, and 6 lack residue 104. Isoforms 2 and 3 lack residues 231–234. Check antibody epitope coverage against these UniProt sequence differences before comparing isoform signals.
That depends on its binding site. Isoform 4 has a changed N terminus; isoform 5 lacks residues 1–17; isoforms 4, 5, and 6 lack residue 104. Isoforms 2 and 3 lack residues 231–234. Check antibody epitope coverage against these UniProt sequence differences before comparing isoform signals.
Which CELF1 modifications matter when interpreting bands?
PTM · The listed canonical UniProt modifications are N-acetylmethionine at position 1, phosphothreonine at 4, and phosphoserine at 179 and 302. Their presence does not establish a visible band shift. State the sequence and numbering convention when comparing these positions with antibody or paper descriptions.
Does this guide establish induction of CELF1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for CELF1?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the M02163-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should CELF1 be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
How does the observed CELF1 band compare with predicted mass?
Interpretation · The observed band is approximately 52 kDa, close to the predicted 52.1 kDa. This agreement supports the expected size but does not establish band identity on its own.
CELF1 is reported in both nucleus and cytoplasm, with RNA-binding activity detected in both. Compare like fractions across samples and report which fraction was measured; a change in one fraction alone need not represent a change in total CELF1.
CELF1 is reported in both nucleus and cytoplasm, with RNA-binding activity detected in both. Compare like fractions across samples and report which fraction was measured; a change in one fraction alone need not represent a change in total CELF1.
Boster reagents
CELF1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-CELF1 Rabbit Monoclonal Antibody
Cat # M02163-1
Real WB data
Anti-CELF-1 Antibody
Cat # A30541
Two the supplier anti-CELF1 antibodies list Western blot images and human, mouse, and rat reactivity. M02163-1 has a detailed blot caption reporting an approximately 52 kDa band in specified human cell and rodent tissue lysates; A30541’s caption provides fewer sample details.
Which to pick: For the documented human cell and mouse or rat tissue samples, consider M02163-1: its WB caption names the lysates, dilution, and observed band. A30541 also has a WB image and lists the same reactivity, but its caption does not identify the cells tested.
Source: BosterBio CELF1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q92879.
- Human Protein Atlas. CELF1 tissue expression.
- PMC3594286 — target-verified WB comparison
- PMC12334686 — target-verified WB comparison
- PMC12621390 — target-verified WB comparison