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Product Info Summary
| SKU: | A30541 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CELF-1 Antibody
SKU/Catalog Number
A30541
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CELF-1 Antibody (Catalog# A30541). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CELF-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30541)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CELF-1. Immunogen sequence location: 71-120
Reactive Species
A30541 is reactive to CELF1 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
52.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30541 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western Blot analysis of various cells using CUG-BP1 Polyclonal Antibody diluted at 1:500
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Western blot analysis of lysates from HeLa and HepG2 cells, using CELF-1 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-CELF-1 Antibody (A30541)
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Customer Q&As
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5 Customer Q&As for Anti-CELF-1 Antibody
Question
We are currently using anti-CELF-1 antibody A30541 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
The anti-CELF-1 antibody (A30541) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-20
Question
Is a blocking peptide available for product anti-CELF-1 antibody (A30541)?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
We do provide the blocking peptide for product anti-CELF-1 antibody (A30541). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-12-03
Question
I have a question about product A30541, anti-CELF-1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30541 anti-CELF-1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-14
Question
I was wanting to use to test anti-CELF-1 antibody A30541 on mouse brain for research purposes, then I may be interested in using anti-CELF-1 antibody A30541 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
The products we sell, including anti-CELF-1 antibody A30541, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-26
Question
Does anti-CELF-1 antibody A30541 work for WB with brain?
S. Taylor
Verified customer
Asked: 2015-08-28
Answer
According to the expression profile of brain, CELF1 is highly expressed in brain. So, it is likely that anti-CELF-1 antibody A30541 will work for WB with brain.
Boster Scientific Support
Answered: 2015-08-28
