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- Table of Contents
Facts about Alpha-crystallin B chain.
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Human | |
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Gene Name: | CRYAB |
Uniprot: | P02511 |
Entrez: | 1410 |
Belongs to: |
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small heat shock protein (HSP20) family |
alpha B crystallin; alpha(B)-crystallin; AlphaB Crystallin; alpha-crystallin B chain; CRYA2; CRYA2alpha crystallin B chain; CRYAB; crystallin, alpha B; CTPP2; Heat shock protein beta-5; heat-shock 20 kD like-protein; HSPB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component
Mass (kDA):
20.159 kDA
Human | |
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Location: | 11q23.1 |
Sequence: | 11; NC_000011.10 (111908564..111923740, complement) |
Lens as well as other tissues (PubMed:838078, PubMed:2387586). Expressed in myocardial tissue (PubMed:28493373).
Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles (PubMed:19464326). Localizes at the Z-bands and the intercalated disk in cardiomyocytes (PubMed:28493373).
What are the most effective uses of CRYAB markers? There are many uses for the CRYAB marker. This includes: applications as well as special samples and species. Boster scientists can submit their research findings and receive product credits. These credits are given to scientists worldwide. Boster Bio The Best Uses of the CRYAB Marker
To assess Boster Bio protein transfer efficiency We first stained the membranes using colloidal gold, which is a highly sensitive stain. We used commercially available stains in this procedure. The results will be an orange or reddish color. The Canon scanner was used to determine optical density. To further stain the membranes we used an enzyme conjugated second antibody. We also measured the efficiency of the transfer of proteins by staining membranes boster bio's 4X Dual Color Buffer for Protein Loading.
The efficiency of membrane transfer depends on three elements that are: the type of gel as well as the molecular weight of the protein being transferred, and the type of membrane used. A lower molecular weight protein is more difficult to transfer to the membrane but one with higher molecular mass will have greater binding capacity. The molecular weight of the protein determines the membrane's sensitivities. For example, a low-molecular-weight protein will be more obvious in WB.
After cleaning the membranes with the reagents, you need to develop autoradiography film by using the appropriate TBS developing fixing buffer. The exposure time can range from just a few seconds to several minutes, based on the desired result. To remove any unbound antibodies, the membrane should be washed three more times using TBS Wash Buffer. The next step of the process is to cover the membranes with either BCIP/NBT or DAB substrate solution and then incubate them in the dark for at least 30 mins.
Boster Bio's protein transfer efficiency using membrane staining kits could be used to determine the effectiveness of proteins or peptides. The antibody solution is then diluted in PBS to make it easier to apply and remove. Once the membranes have dried, they can then be examined using a fluorescent microscope or digital camera. The analysis shows that the Boster biosystem can precisely measure protein transfer efficiency.
The most popular method of protein transfer is the method of wet transfer. In this method the sandwich is created by utilizing a filter paper soaked with buffer. It is then inserted into a cassette for transfer. The cassette is submerged in a tank that is filled with buffer. Although the process takes overnight, it is quick and consistent. However it is more prone to bubbles and slow. This process is also prone to clumping, and also to transfer too many proteins.
In addition to the Boster Bio Protein Transfer Efficiency through Membrane Staining We also offer custom immunostaining of tissues. Boster's Western Blot service comprises the complete protein extract from tissues, the use of any antibody, and quantitative analysis. All of this information is provided in high-quality digital images. You will benefit from the Boster Bio protein-transfer efficiency by membrane staining.
The functions of the CRYAB Marker is largely contingent on the state of the disease. Triple-negative breast cancer has more expression of the marker. It is found in basal cells of normal epithelium, and is significantly increased in these kinds of cancers. The CRYAB Marker is detectable by immunohistochemistry (IHC) methods in breast cancer samples. CRYAB expression can be used to determine whether patients have advanced or early-stage cancer.
Other uses for the CRYAB marker include the diagnosis of epileptic foci in children. It is also associated to the longevity of retinal ganglion cells after axotomy (amputation of the optic nerve) in mice. CRYAB has been linked to motor skills recovery as well as secondary tissue injury following spinal cord injury. It's a great marker for a variety of research purposes. In this article, we'll look at some of these uses of the CRYAB Marker.
One of the main functions of the CRYAB Marker involves monitoring the production of proinflammatory cytokines in cancer cells. THP-1 cells were cultivated in vitro, transfected by an lentivirus that expressed CRYAB, and stimulated by LPS. The production of proinflammatory cytokines were measured using qRT-PCR as well as ELISA. The CRYAB-expressing Lentivirus significantly reduced proinflammatory cytokine production.
The gene aB–crystallin encodes aB–crystallin. It is found in both cardiac and skeletal muscles. It is found in Z-bands alongside desmin and interacts with it via interactions. It also serves as a heat shock protein, stabilizing filaments of desmin under stress. It also has a high-conserved arginatine residue at its N-terminal.
The interaction between DES-Synemin and DES-Synmin is a method for protein detection. It utilizes antibody-antigen interactions in order to determine specific cellular components. Researchers improve the preparation of samples and staining procedures to create a powerful signal that is easy to interpret. DES and Synemin interact at a variety of sites in cells, including Z lines adheens junctions, cell membranes. This article will explore some of the possible interactions between these proteins.
The company also makes antibodies for research and development. These kits are able to detect biomarkers in cancer, neurosciences inflammation, cancer, and the development of biology. These antibodies are highly sensitive and specific to a picogram. Boster rewards first reviewers with product credits. Boster rewards scientists worldwide. The company is committed to advancing the field of molecular biology and chemistry. Boster's antibody kits are available at Tebu-bio.
PMID: 838078 by Kramps J.A., et al. The primary structure of the B2 chain of human alpha-crystallin.
PMID: 2387586 by Dubin R.A., et al. Human alpha B-crystallin gene and preferential promoter function in lens.